Zeiss Axioskop 2 plus Operating Manual page 108

Carl Zeiss
Fig. 3-27 Components for Epi-fluorescence
on the Axioskop 2 plus
• Either open the luminous-field diaphragm until it just disappears behind the edge of the field of view
or, if there is a risk of specimen bleaching, reduce it until it is visible in the field of view.
• Finally, refocus on the specimen and optimize the HBO 103 collector position as described in section
2.1.11. Set the collector in such a way that homogeneous illumination is obtained with the short-wave
excitation reflector module. When modules with long-wave excitation are used, correction of the
collector position is not required.
3-40
OPERATION
Illumination and contrasting techniques
4 0
9 0
• Switch on the HBO 103 or HBO 50 mercury
vapor short-arc lamp (3-27/2) and allow it to
heat up to its operation temperature for
approx. 15 mins.
• Select the FL P&C reflector module in reflector
turret (3-27/1)
fluorescence filter combination (depending on
the excitation) and switch it on.
• When using the incident-light filter slider
(3-27/4), unblock the light path in the incident-
light illuminator.
• Remove one eyepiece from the tube and set the
aperture diaphragm (3-27/3) by opening it until
the entire objective exit pupil is unblocked.
• Insert eyepiece in tube again and close
luminous-field diaphragm (3-27/5) until it is
visible in the field of view.
• Use the two centering screws (3-27/6) to center
the luminous-field diaphragm in relation to the
edge of the field of view.
Axioskop 2 plus
Axioskop 2 mot plus
containing the required
B 40-075 e 02/01