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Zeiss Lightsheet Z.1 Manuals
Manuals and User Guides for Zeiss Lightsheet Z.1. We have
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Zeiss Lightsheet Z.1 manual available for free PDF download: Operating Manual
Zeiss Lightsheet Z.1 Operating Manual (427 pages)
ZEN 2012 (black edition)
Brand:
Zeiss
| Category:
Microscope
| Size: 55.15 MB
Table of Contents
Hardware
5
Table of Contents
5
Chapter 1 - Hardware
7
Maintenance and Cleaning
7
Maintenance of the Liquid Cooling System
9
Ergodrive Operating Panel
10
User Interfaces
13
Installation and Deinstallation of the Detection Modules
14
Adjustment – Detector Recognition
19
Adjustment – Manual Detector Alignment
21
Adjust the Grating Focus for the Automatic or Manual Detector Alignment Tool
23
Cable Connections for the Detection Module "Pco.edge
26
Cable Connections for the Detection Module "Standard
27
Assembly of the Sample Chamber
28
Assembly of the Sample Chamber Body and the Sample Chamber Dove Tail Slide
31
Insertion of the Drain Connector, Luer-Lock Connectors and Blind Plugs
33
Insertion of Accessories for Incubation
34
Removing and Inserting the Sample Chamber
35
Assembly of the Sample Holder
36
Assembly of Sample Holder for Syringes
38
Inserting and Removing the Sample Holder
39
Installation of the Incubation Modules
41
CO -Module Lightsheet Z.1
43
Heating Device Humidity S1
45
Switch Incubation on and off
47
Removing and Inserting the Illumination Optics Unit
49
Removing and Inserting the Detection Optics Unit
50
Removing and Inserting the Reflector Turret for Emission Selection
51
Removing and Inserting the Reflector Turret for Laser Blocking Filter
53
Index
56
Troubleshooting
93
Table of Contents
172
Shutdown Procedure
180
Chapter 4 - System Operation
207
Left Tool Area and Hardware Control Tools
207
Locate Tab
207
Locate Capillary
208
Locate Sample
208
Specimen Navigator Tool
209
Incubator Tool
210
Acquisition Tab
211
Experiment Manager
212
Action Buttons
213
Multidimensional Acquisition Settings
214
Setup Manager - Laser
216
Setup Manager - Light Path
217
Track Definition
218
Transmitted Light Lamp Control
219
Laser Control
219
Incubator
219
Detection Optics Display
220
Laser Blocking Filter Selection
220
Emission Selection Filter Selection
220
Channel Selection
221
Specimen Navigator Tool
222
Acquisition Parameter - Acquisition Mode
223
Acquisition Parameter - Channel
227
Defining Channels and Tracks
227
Defining Detector and Illumination Settings
231
Defining Display Options
234
Acquisition Parameter - Incubator
235
Multidimensional Acquisition - Z-Stack
236
Performing a Z-Stack Using First/Last Mode
236
Performing a Z-Stack Using Center Mode
238
Multidimensional Acquisition - Time Series
239
Interval Time
241
Marker
242
Start
244
End
245
Multidimensional Acquisition - Multiview-Setup
246
Multidimensional Acquisition - Information on Experiment
251
General Structure of the Processing Tab
252
Processing Tab
252
Processing - Maximum Intensity Projection
256
Processing - Color-Coded Projection
257
Processing - Image Calculator
258
Processing - Average
260
Processing - Filter
261
Processing - Linear Unmixing
264
Processing - Ion Concentration
269
Single Wavelength Dyes - Offline Calibration
271
Ratiometric Dyes
272
Processing - Correlation
275
Processing - Modify Series
276
Processing - HDR-Imaging
277
Processing - Stitch
281
Processing - PALM
283
Palm - Palm
284
PALM - Remove PALM Outliers
291
PALM - Import PALM Molecules
292
PALM - Convert to Image
293
Processing - Particle Tracking
294
Processing - Structured Illumination
295
Structured Illumination - Structured Illumination
296
Structured Illumination - TIRF-Filtered
301
Structured Illumination - Experimental PSF
303
Structured Illumination - Fourier Transform
306
Processing - Channel Alignment
307
Processing - Lightsheet Processing
312
Dual Side Fusion
312
Multiview Processing
313
Online Multiview Processing
322
Processing - Copy
323
Processing - ICS
325
Remove Structure
326
ICS Correlation
327
Map Filter
328
Processing - Adjust
329
Burn in Brightness and Contrast
329
Interpolate Brightness and Contrast
330
Channel Shift
331
Shading Correction
332
Maintain - Objectives
333
Detection Optics
334
Select Detection Optic
334
Add Objective
335
Remove Objective
336
Show/Edit Objective
336
Illumination Optics
336
Maintain Tab
333
Maintain - Filters
337
Maintain - System Options
338
System Options - Load Experiment Manager
338
System Options - Reuse
339
System Options - Hardware
340
System Options - Image Display
340
Maintain - Streaming
341
Maintain - Adjustment
342
Adjustment - Detector Recognition
342
Adjustment - Automatic Detector Alignment
343
Adjustment - Manual Detector Alignment
345
Center Screen Area / Image Containers - Display and Image Analysis
348
Structure and Functional Concept of the Center Screen Area and the Image Display Container
348
General Structure
348
Container Configuration with the Container Context Menu
353
View
354
Dimensions
354
Display
358
Player
359
Graphics
360
Preview View
363
Split View
364
Gallery View
365
Ortho View
366
Ortho - Select Function
367
Ortho - Distance Function
368
Cut View
369
D View
370
View (3D Visart)
372
Shadow Projection
374
Transparency Render Mode
377
Maximum Mode
378
Surface Render Mode
378
Mixed Render Mode
379
Clipping Planes
380
Flying Mode
384
3D Rendering Settings in Visart
384
Series
386
Interactive Measurements
388
Settings
389
Options
390
View - Basic
391
Histogram View
392
Colocalization View
395
How a Scatter Diagram Is Generated
397
Colocalization Coefficients
398
Quantitative Colocalization Parameters Shown in the Data Table
398
Overlap Coefficient after Manders
399
Profile View
399
Mean of ROI: Additional View Type for Time Series
402
Information View
405
General
406
Right Tool Area, Data Management and Storage
406
Comments on Data Management
407
ZEN File Browser
409
Gallery View of the ZEN File Browser
410
Form View of the ZEN File Browser
413
Images and Documents Panel
416
Opening of Files Via the "Open" Command in the File Menu
419
Save
419
Export of Images
420
Index
421
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