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DISK SCANNING BIOLOGICAL MICROSCOPE Petition Thank you for your adoption of OLYMPUS microscope, this time. Please read this manual carefully in order to get optimum performance of this microscope and for safety considerations. When using microscope, please have this manual by you and keep it with care.
Manual conventions.................. 6 I. System Outline 1 SYSTEM OUTLINE............1-1 1-1 Principle.................... 1-1 1-2 Features .................... 1-2 1-3 System Configuration..............1-3 1-3-1 In case of BX series ..................1-3 1-3-2 BX-DSU .......................1-4 1-3-3 In case of IX series ..................1-5 1-3-4 IX2-DSU.......................1-6...
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CONTENTS II. DSU 2 SUMMARY OF OBSERVATION PROCEDURES ..2-1 3 DETAILS OF EACH OPERATION........ 3-1 3-1 General Precaution for observation..........3-1 3-2 Selection of fluorescent mirror unit..........3-2 3-3 Table of Combination – Objective lens to each observation and applicable disk .................
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CONTENTS 4-1-2 Simultaneous observation of reflected-light fluorescent and transmitting Nomarski differential interference..............4-2 4-2 Visual observation (IX) ..............4-4 4-2-1 Simultaneous observation of reflected-light fluorescence and phase contrast4-4 4-2-2 Simultaneous observation of reflected-light fluorescence and differential interference (transmitting) ................4-5 4-3 Camera observation (BX-IX) ............4-6 4-3-1 Transmitting bright-field and transmitted light phase contrast observation ...4-6 4-3-2 Transmitting differential interference observation.........4-6 5 TROUBLESHOOTING GUIDE ........
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CONTENTS 8-9 Installation of front side turret............8-10 8-9-1 In case of BX .....................8-10 8-9-2 In case of IX....................8-10 8-10 Installation of visual mirror unit (front side) ......8-11 8-10-1 In case of BX ...................8-11 8-10-2 In case of IX.....................8-12 8-10-3 Method to make optionnal fluorescent mirror unit........8-13 8-11 IN/OUT of rear side turret ............
INTRODUCTION INTRODUCTION Be sure to read this section for safe use of the equipment. Caution This manual – part or whole, shall not be used or reproduced without permission. Suggestions to unpacking Release of transportation lock for disk unit Note When powering up the control box without removing transportation lock knob, the disk unit may be damaged.
INTRODUCTION Loosen knob (5) of disk unit (at 2 places) and open disk protective covers (6). Loosen transportation lock knob (7) and remove it from the disk unit (4). Install the transportation lock knob you removed to the threaded hole (8) of disk unit. The transportation lock knob may be used for re- Note transportation.
INTRODUCTION Do not turn the lamp ON without installing lamp house to microscope. Ultraviolet ray of lamp is hazardous to your eyes. Do not look at the light from lamp house directly. Do not look at excited light directly. When you use microscope, attach ultraviolet ray cutting plate and see specimen and others through ultraviolet ray cutting plate for sure.
INTRODUCTION Warning labels Warning label is attached to the place where caution is particularly required for operation. Observe the instructions. Lamp house mount adapter (Hot Surface caution) Disk box (Entanglement caution) When warning label is blurred or peeled off, contact our local sales office for change. Page...
INTRODUCTION When using the system Handle this system with care so as not to give a shock as it is a precision instrument. This system requires independent fluorescent mirror unit for visual and camera observation respectively. Install fluorescent mirror unit for visual observation to front side turret and install fluorescent mirror unit for camera observation to rear side turret.
INTRODUCTION Manual conventions This manual describes with use of the following manual conventions. ² Conventions of note, reference and point Conventions Description Indicates note to prevent injury of user and damage of product (including home furniture, etc.). Typical note is indicated with Note Note Advice or hint for reference is indicated with...
It acquires a high contrast image through resolution enhanced in light axis direction very rapidly. In addition, Olympus motorized microscopes IX81, BX61WI, BX61 or BX62 and their related motorized units may be controlled through a 3rd party software, which is designed for observation by CCD camera.
SYSTEM OUTLINE / Features 1-2 Features ● Confocal disk employed enables to acquire image of confocal effect. ● As while light source such as mercury or xenon is used as a light source instead of laser, excited light of various wavelengths can be used by combination of excitation filter and cube.
SYSTEM OUTLINE / System Configuration 1-3 System Configuration 1-3-1 In case of BX series Microscope (BX51, BX52, BX61, BX62, BX51WI or BX61WI) Cooled CCD Camera DSU Disk box (DU-DBIX) Motorized filter wheel (U-FWR) Light illuminator Shutter and for DSU BX Filter wheel (DU-RFABX) Lamp...
SYSTEM OUTLINE / System Configuration Table of Instruction Manuals for Modules to be integrated Instruction Manual Module name SYSTEM MICROSCOPE BX51/BX52 MOTORIZED SYSTEM BX61/BX62 MICROSCOPE FIXED-STAGE UPRIGHT BX51WI MICROSCOPE FIXED-STAGE MOTORIZED BX61WI UPRIGHT MICROSCOPE CONTROL BOX FOR IX2 HAND IX2-UCB SWITCH U-HSTR2 Cooled CCD Camera...
SYSTEM OUTLINE / System Configuration 1-3-3 In case of IX series DSU Disk box Cooled CCD Camera (DU-DBIX) Light illuminator for DSU IX Motorized filter wheel (DU-RFAIX) (U-FWR) Microscope (IX71, IX81) Shutter and Filter wheel Lamp Monitor House Computer DSU Hand switch (DU-HSW) Hand switch (U-HSTR2)
* Light illuminator for DSU IX * DSU motor drive board * Mirror unit cassette (DU-RFAIX) (DU-MTDRV) IX2-DSU includes the units, which have a mark "*" in its description on the above picture. I . System Outline I . 1 - 6 Page...
SUMMARY OF OBSERVATION PROCEDURES 2 SUMMARY OF OBSERVATION PROCEDURES This section describes procedures for fluorescent observation by visual method followed by camera observation by confocal mode. Preparation •Install fluorescent mirror unit and objective lens applicable to observation method. •Do centering of mercury lamp or xenon lamp. •Bring camera in focus.
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Summary of observation procedures (From previous page) Turn turret of rear side and put camera mirror unit ......Camera mirror unit (rear 3-10 that suits to the specimen. side) change button Turn turret of front side and put No.1 reference ......
DETAILS OF EACH OPERATION / General Precaution for observation 3 DETAILS OF EACH OPERATION 3-1 General Precaution for observation Verify that input voltage/frequency to be used is within the range of that described in identification plate of the system. Verify that power cord as well as connecting cord is securely connected. In case that transmitted light phase contrast observation or transmitted light DIC (Differential Interference Contrast) observation only is required, make one place where fluorescent mirror unit is not attached and do observation.
DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit Be careful that specimen of certain kind cannot use anti-color Note deterioration agent. 3-2 Selection of fluorescent mirror unit Select fluorescent mirror unit that has excitation to meet with coloring matter. Do not use fluorescent mirror unit in combination with brightfield Note of view mirror unit (U-MBF3).
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DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit Table of combination for mirror unit and dichroic mirror filter Excitation Excitation Mirror Unit Dichroic Mirror Barrier Filter Fluorochrome Method Filter U-MWU2 BP330-385 • Auto fluorescence observation • DAPI Dyeing: DNA DM400 BA420 •...
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DETAILS OF EACH OPERATION / Selection of fluorescent mirror unit Color Separation Filter Combinations Excitation Excitation Mirror Unit Dichroic Mirror Barrier Filter Fluorochrome Method Filter Observation of U-excited coloring matter only when Dyeing U-excited U-MNUA2 DM400 BP360-370 BA420-460 coloring matter and multi-Dyeing of FITC.
DETAILS OF EACH OPERATION / Table of Combination – Objective lens to each observation and applicable disk 3-3 Table of Combination – Objective lens to each observation and applicable disk Reflected light fluorescence Differential interference Phase Objection lens Applicable disk contrast U, V, BV B, IB, G, IY...
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DETAILS OF EACH OPERATION / Table of Combination – Objective lens to each observation and applicable disk Reflected light fluorescence Differential interference Phase Objection lens Applicable disk contrast U, V, BV B, IB, G, IY BX2WI* LCPlanFl – – – –...
DETAILS OF EACH OPERATION / Assignment of operating buttons on body (BX61, BX62 or BX61WI only) 3-4 Assignment of operating buttons on body (BX61, BX62 or BX61WI only) When IX2-BSW software boots up, microscope IX and its operation buttons will be displayed on tab called [Frame SW]. When you use BX61, BX62 or BX61WI, however, microscope does not appear.
DETAILS OF EACH OPERATION / Powering up Focus retreat button F/C Change button Lamp ON/OFF button Lamp set* Not used* In case that control box (IX2-UCB) is of type 1, the identifying plate indicates IX2-UCB. In case that control box (IX2-UCB) is of type 2, the identifying plate indicates IX2-UCB-2. 3-5 Powering up Switch on the control box.
DETAILS OF EACH OPERATION / Changeover of visual mirror unit (front side) 3-7 Changeover of visual mirror unit (front side) 3-7-1 In case of BX Turn turret (1) located in front of light illuminator and insert mirror unit desired or reference mirror into light path. Reference mirror to insert into light path is normally put in No.
DETAILS OF EACH OPERATION / Disk IN/OUT 3-8 Disk IN/OUT When you wish to insert disk into light path, press left IN button of DSU hand switch. Likewise, press right OUT button so that the disk would be displaced from light path.
DETAILS OF EACH OPERATION / Changeover of camera mirror unit (rear side) 3-10 Changeover of camera mirror unit (rear side) Press button on hand switch to turn the turret and insert mirror unit desired or reference mirror into light path. Reference mirror is put in No. 1. Buttons to designate mirror unit are assigned at ex-factory as illustrated in left figure.
DETAILS OF EACH OPERATION / Adjustment of field iris diaphragm 3-12 Adjustment of field iris diaphragm Turn front side turret (1) and insert fluorescent mirror unit desired into light path. Turn rear side turret and insert reference mirror of No. 1 into light path.
DETAILS OF EACH OPERATION / Lamp centering 3-13 Lamp centering Lamp centering should be done after arc image of lamp is stablized after main switch was turned ON (5 to 10 minitues later after lamp ON). In case that frost filter 32FR80 is used, displace it from light path.
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DETAILS OF EACH OPERATION / Lamp centering 11. Turn lamp centering knob (4) and overlap arc image and mirror arc image. (D) When you feel that the collector lens focusing knob (3) is hard to turn as it is located far away, insert extention handle U-CLA (available at option) into the knob.
DETAILS OF EACH OPERATION / Camera focus alignment 3-14 Camera focus alignment Bring a live image on monitor. Put objective lens 10x into light path and displace the specimen from light path. Press left IN button (1) of DSU hand switch and put the disk into light path.
VARIETIES OF OBSERVATION METHODS / Visual observation (BX) 4 VARIETIES OF OBSERVATION METHODS 4-1 Visual observation (BX) This system can be used for not only reflected-light fluorescent observation but also it combines transmitting bright-field observation, transmitted light phase contrast observation and transmitting differential interference observation.
VARIETIES OF OBSERVATION METHODS / Visual observation (BX) 4-1-2 Simultaneous observation of reflected-light fluorescent and transmitting Nomarski differential interference Transmitting Nomarski differential interference observation requires the following units. (1) Universal condenser (U-UCD8) (2) Transmitting DIC slider (U-DICT, U-DICTS, U-DICTHR or U-DICTHC) (3) Analyzer (U-AN or U-AN360-3) (4) Revolving nosepiece or motorized revolving nosepiece for DIC.
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VARIETIES OF OBSERVATION METHODS / Visual observation (BX) When change of reflected-light fluorescence and transmitting Nomarski differential interference is frequently done and simultaneous observation is also required, it is recommended that high abrasion-resistant analyzer slider (U-ANH) is used instead of analyzer (U-AN). When change of reflected-light fluorescence and transmitting Nomarski differential interference is frequently done but simultaneous observation is not required, it would be convenient if mirror unit (U-MDICT3) for...
VARIETIES OF OBSERVATION METHODS / Visual observation (IX) 4-2 Visual observation (IX) This system can be used for not only reflected-light fluorescent observation but also it combines transmitting bright-field observation, transmitted light phase contrast observation and transmitting differential interference observation. Particularly, for specimen that color deterioration occurs earlier, the color deterioration can be minimized by initial positioning through phase contrast observation or differential interference observation.
VARIETIES OF OBSERVATION METHODS / Visual observation (IX) 4-2-2 Simultaneous observation of reflected-light fluorescence and differential interference (transmitting) Differential interference observation requires the following units. (1) One of condenser, IX2-LWUCDA, IX2-LWUCD or U-UCD8. (2) One of differential interference slider, U-DICT, U-DICTS, U-DICTHC or U-DICTHR (3) Analyzer, U-ANT or IX2-AN The analyzer IX2-AN for simultaneous observation is put in lower insertion port below the dichroic mirror in order to use reflected-light...
VARIETIES OF OBSERVATION METHODS / Camera observation (BX-IX) 4-3 Camera observation (BX-IX) This system provides reflected-light fluorescent observation and also, by changeover of rear side turret, it enables camera observation for transmitting bright-field observation, transmitted light phase contrast observation and transmitting differential inteference observation. 4-3-1 Transmitting bright-field and transmitted light phase contrast observation To execute camera observation for each transmitting, the system should be arranged for visual observation as described in previous section, 4-1 and 4-2.
Depending upon how the system is used, it may not exhibit adequate performance of the system, though it may not be a failure. When trouble occurs, exercise adequate countermeasure with reference to the following table. In case that trouble has not been improved, please contact local Olympus representative for assistance. Trouble...
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TROUBLESHOOTING GUIDE Trouble Cause Countermeasure Confocal image is Filter like that is entering Displace unnecessary substance from light 3-11 extremely dark. light path. path. Exposure time of camera Adjust to adequate time. Instruction is not correct. manual of camera used Range of camera Adjust to adequate level.
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TROUBLESHOOTING GUIDE Trouble Cause Countermeasures Ref. Flare is remarkable. Ambient light (such as Turn off fluorescent lamp or cut ambient – fluorescent lamp) is light with use of specimen cover. entering light path. Exposure time of camera Adjust to adequate time. Instruction is not correct.
SPECIFICATIONS / BX Series 7 SPECIFICATIONS 7-1 BX Series Item Specifications Lens barrel magnification Number of views Visual observation side: FN22mm (In reflected illumination, peripheral light intensity may be insufficient if objective lens power is of 20x or less) Camera obervation side: FN11mm Shutter Knob at front side turret cover.
SPECIFICATIONS / IX Series 7-2 IX Series Item Specifications Lens-barrel magnification Number of views Visual observation side: FN22mm (In reflected illumination, peripheral light intensity may be insufficient if objection lens is of 20x or less) Camera observation side: FN11mm Shutter Knob at front side turret cover.
ASSEMBLY / Installation of light illuminator 8-2 Installation of light illuminator 8-2-1 In case of BX Remove caps of mounting screws (2) on top of light illuminator (1) with use of tweezers. When using BX61, BX62, BX51 or BX52, put arm lift kit (WI- ARMAD) on the arm and fix mounting screws (3) (4 pieces) to the the Allen wrench that light illuminator firmly, using dedicated...
ASSEMBLY / Installation of disk box 8-2-2 In case of IX Loosen fixing screws (1) (2 places) that fix light illuminator to microscope frame, using the Allen wrench. Insert light illuminator (2) into mounting hole (3). (both sides) Observe that pin at lower part of light illuminator is aligned with mounting hole slot.
ASSEMBLY / Removal of transportation lock 8-4 Removal of transportation lock This work should be done before connecting Note cable of disk box. Loosen screws (2) (2 places) located on top of disk box to fasten at front side of access panel to microscope frame and detach the access panel (1).
ASSEMBLY / Installation of illuminator 8-5 Installation of illuminator Loosen fixing screws (1) (2 places) that fix disk box to microscope frame, using the Allen screwdriver. Insert illuminator (2) into disk box (3) and push it to the dead end. At this time, attach it in a way that FS unit faces towards observer.
ASSEMBLY / Installation of mercury lamp 8-7 Installation of mercury lamp Loosen socket fixing screw (1), using the Allen screwdriver. Hold upper part of lamp house and pull it vertically to detach socket section. If you hold centering knob (2), it may cause a failure. Note Place socket section upside down as illustrated.
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ASSEMBLY / Installation of mercury lamp Life meter setting Reset button (1) is located at front panel. Press center part (2) of this button and make the display to 000.0. Display – 000.0 means time. Life of lamp – USH102D is 200 hours, while HBO103W/2 is 300 hours.
ASSEMBLY / Lamp power supply setting 8-8 Lamp power supply setting Cord or cable is weak against bend or twist. Care must be taken so as not to apply excessive force. When connecting power cord, turn main siwtch to mark side (OFF).
ASSEMBLY / Installation of front side turret 8-9 Installation of front side turret 8-9-1 In case of BX (Removal of turret). Loosen fixing screw (1) from hole (1) located at right side of light illuminator and pull out turret (2) forward. (Installation of turret).
ASSEMBLY / Installation of visual mirror unit (front side) 8-10 Installation of visual mirror unit (front side) 8-10-1 In case of BX Turn turret upside down and loosen fixing screw (1), using dedicated the Allen screwdriver and remove dummy mirror unit (2) (remove the dummy mirror unit only where a mirror unit is to be installed from now on).
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ASSEMBLY / Installation of visual mirror unit (front side) 8-10-2 In case of IX Remove cover (1) that is sucked with magnet from mirror unit cassette. When mirror units already exist inside the cassette, be Note careful so as not to touch dichroic mirror or excitation filter with your finger.
ASSEMBLY / Installation of visual mirror unit (front side) 8-10-3 Method to make optionnal fluorescent mirror unit You can make optional fluorescent mirror unit by attaching mirror unit frame U-MF2 to commercially available barrier Filter, excitation filter or dichroic mirror. Dimensional conditions for optical parts •...
ASSEMBLY / IN/OUT of rear side turret 8-11 IN/OUT of rear side turret When you install or detach the turret, you must do the works Note after you turn the power of control box. (Removal). Loosen screws (2) (2 places) at rear side of access panel (1) on top of disk box, using dedicated the Allen screwdriver and remove the access panel.
ASSEMBLY / Disk installation and change For camera observation, use fluorescent mirror Note unit of HQ type. The fluorescent mirror unit type DSU-MRFPHQ – accessory of this system – is dedicated for camera observation. 8-13 Disk installation and change For disk installation or change, you must do the works after you Note verify that the disk entered into light path and then, you turn the power of control box.
ASSEMBLY / Installation of light shielding tube (BX61, BX62, BX51 or BX52) Set the disk, putting the side attached with name seal (dark blue side) down and, holding edge of disk, turn rotary shaft (7) in counter- clockwise direction to screw in to the end, using the Allen screwdriver. After fixing, remover the wrapper.
ASSEMBLY / Installation of UV-cut plate 8-15 Installation of UV-cut plate 8-15-1 In case of BX Put UV-cut plate underneath the front side mirror turret as illustrated. 8-15-2 In case of IX Loosen fixing knob (1) for UV-cut plate. Put the UV-cut plate in receptacle of lens barrel of microscope frame as illustrated.
ASSEMBLY / Filter IN/OUT inside motorized filter wheel 8-17 Filter IN/OUT inside motorized filter wheel At ex-factory, filters are attached as shown in the following table. When you wish to change the setting, follow to the following procedures. No.1 No.2 No.3 No.4 No.5...
ASSEMBLY / Field iris diaphragm IN/OUT Insert presser ring driver (4) into slots (5) of presser ring and turn it in clockwise direction to fix the filter (6). When you attach filter of 25mm diameter to conversion adapter, use small side of presser ring driver (4). Be careful when you attach conversion adapter as the portion to insert filter is slanted by 5°.
ASSEMBLY / Installation of camera adapter and filter 8-19 Installation of camera adapter and filter 8-19-1 In case of DSU C-mount adapter Loosen fixing screw (1), using the Allen screwdriver. Insert C-mount adapter (3) into disk box (2). Tighten fixing screw (1), using the Allen screwdriver. 8-19-2 C-mount adapter for DSULAMBDA10 / C-mount adapter for Ludl In case of LAMBDA10 Screw in the upper adapter and lower adapter by turning them into...
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ASSEMBLY / Installation of camera adapter and filter Loosen fixing screw (7) of disk box, using the Allen screwdriver. Insert C-mount adapter that is attached with filter wheel into mount (8) of disk box. Tighten fixing screw (7), using the Allen screwdriver. In case of Ludl Open lid of filter wheel and remove adapter that is attached from the beginning, using Philips type + screwdriver.
ASSEMBLY / Installation of camera 8-20 Installation of camera Loosen fixing screw (1) of port, using the Allen screwdriver that comes as accessory for microscope frame and remove C-mount adapter (2) inside the port. Screw in and fix the C-mount adapter(2) that was removed to C-mount section (3) of camera firmly.
ASSEMBLY / DIP switch settings on control box (BX61, BX62 and BX61W1 only) 8-21 DIP switch settings on control box (BX61, BX62 and BX61W1 only) DIP switch settings should be done after power of control box is Note turned OFF. When you use BX61, BX62 or BX61W1, you must change DIP switch settings on control box.
ASSEMBLY / Z board setting and installation 8-23 Z board setting and installation 8-23-1 In case of BX61 and BX62 For Z board, use type U-ZPCB(T2) for sure. Note In case that U-ZPCB(T2) or subsequent is not used, the following troubles may occur even if correct setting was done.
ASSEMBLY / Z board setting and installation Using knobs you removed, fix Z board (2). Likewise, attach the other cover (3). Keep one piece of slot cover that remained with care as it Note may be required for Z board when it is removed. 8-23-2 In case of BX61W1 For Z board, use type U-ZPCB(T2) for sure.
ASSEMBLY / Z board setting and installation Z board installation You must install Z board after you turn power of control box Note to OFF. Loosen fixing knobs (6 pieces) for two slot covers at back side of control box (1) and remove covers and knobs. Aligning direction of connector of control box and connector of Z board (2), insert board along with board rails.
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ASSEMBLY / Z board setting and installation Setting change of ONBOARD DIP SW Use DIP SW set to OFF except the following DIP SW. Note Turn DIP SW that is to be changed only to ON . S3 Changes NO.4 and NO.5 to ON. Note If DIP switches are not correctly set, objective lens comes down and hits the specimen.
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2 Corporate Center Drive, Melville, NY 11747-3157, U.S.A. OLYMPUS SINGAPORE PTE LTD. 491B River Valley Road, #12-01/04 Valley Point Office Tower, Singapore 248373 OLYMPUS OPTICAL CO. (U.K.) LTD. 2-8 Honduras Street, London EC1Y OTX, United Kingdom. OLYMPUS AUSTRALIA PTY. LTD.
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I have an Olympus IX70 and I my "burner on" bottom is blinking. Can you please help me troubleshoot the issue?