Andor Technology iXon Ultra 897 User Manual page 159

TUTORIAL 3
(such as was recorded in video mode) in order to determine the position of
the sides of a sub area (square or rectangle) encompassing the fluorescent
beads of interest. In the Setup Image tab of the Setup Acquisition Dialog
box, select Custom Sub-Image and enter the sub-area dimensions into the
left, right, top and bottom boxes. In the Setup CCD tab, the new frame rate
created under these conditions may be viewed.
Then, place ROI areas over the immobilised spots and create kinetic plots
of the mean values vs. time as described above. The plots should readily
represent the fluctuations in intensity over time as the attenuating action
was performed. This observation may be considered as representative of the
fluctuations in intensity that is commonly observed for single fluorescing
molecules.
Section 7. 3
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