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Thermo Fisher Scientific
Heraeus Megafuge 16
Heraeus Megafuge 16R
Instruction manual
50120972-5
June 2011

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  • Page 1 Thermo Fisher Scientific Heraeus Megafuge 16 Heraeus Megafuge 16R Instruction manual 50120972-5 June 2011...
  • Page 2 © 2011 Thermo Fisher Scientific Inc. All rights reserved. BIOLiner , BIOShield , HIGHPlate , HIGHConic , AutoLock FIBERLite and CLINIConic are either registered trademarks or trademarks of Thermo Fisher Scientific. All other trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries. Thermo Fisher Scientific Inc.
  • Page 4 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96. It is marked by the following symbol: Thermo Fisher Scientific has entered into agreements with recycling and disposal companies in all EU Member States for the recycling and disposal of this device.
  • Page 5: Table Of Contents

    Entering Parameters ....................4-4 Saving programs ...................... 4-6 Centrifugation ......................4-7 Temperature Adaptation during Standstill ............... 4-8 Short-term Centrifugation ..................4-8 Removing the Rotor ....................4-8 Aerosol-tight Rotors ....................4-9 Aligning the Centrifuge ................... 4-9 Thermo Scientific Megafuge 16 / 16R...
  • Page 6 Service of Thermo Fisher Scientific ................6-5 Chapter 7 Trouble Shooting ........................7-1 Mechanical Emergency Door Release ..............7-2 Troubleshooting by User ..................7-3 When to contact Customer Service ................7-5 Chemical Compatibility Chart ................A-1 Contact Information ....................B-1 Megafuge 16 / 16R Thermo Scientific...
  • Page 7: Preface

    Failure to follow the instructions and safety information in this instruction manual will result in the expiration of the sellers warranty. Scope of Supply Article Number Quantity Check Zentrifuge Heraeus Megafuge 16/16R Power supply cable 50120972 Instruction manual 70009824 Anti-corrosion oil If any parts are missing, please contact your nearest Thermo Fisher Scientific representative.
  • Page 8: Accident Prevention

    Preface Accident Prevention Prerequisite for the safe operation of the Heraeus Megafuge 16/16R is a work environment in compliance with standards, directives and trade association safety regulations and proper instruction of the user. The safety regulations contain the following basic recommendations: •...
  • Page 9 IF A HAZARDOUS SITUATION OCCURS, TURN OFF THE POWER SUPPLY TO THE CENTRIFUGE AND LEAVE THE AREA IMMEDIATELY. Intended Use The centrifuge is to be used for separating materials of different density or particle size suspended in a liquid. Thermo Scientific Megafuge 16 / 16R...
  • Page 10 CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. Megafuge 16 / 16R Thermo Scientific...
  • Page 11: Introduction And Description

    Introduction and Description Contents “Characteristics” page 1-2 • • “Technical Data” page 1-3 “Directives, Standards and Guidelines” page 1-4 • • “Functions and Features” page 1-5 “Mains Supply” page 1-5 • • “Rotor Selection” page 1-6 Thermo Scientific Megafuge 16 / 16R...
  • Page 12: Characteristics

    Introduction and Description Characteristics Characteristics The Heraeus Megafuge 16/16R is an in-vitro diagnostics device according to the In-Vitro Diagnostics Directive 98/79/EC. Several rotors with a wide range of tubes can be used. The set speed is reached within seconds. The maintenance-free induction motor ensures quiet and low-vibration operation even at high speeds, and guarantees a very long lifetime.
  • Page 13: Technical Data

    Introduction and Description Technical Data Technical Data The technical data of the Heraeus Megafuge 16/16R is listed in the following table. Table 1-1. Technical Data Heraeus Megafuge 16/16R Feature Value Environmental conditions -Use in interior spaces -Altitudes of up to 2,000 m above sea level -max.
  • Page 14: Directives, Standards And Guidelines

    100V 50Hz IEC 61010-1 2 Edition IEC 61010-2-020 2 Edition IEC 61010-2-101 EN 61326-1 EN 61326-2-6 EN 55011A EN 61000-6-2 EN ISO 13485 Megafuge 16 / 16R Thermo Scientific...
  • Page 15: Functions And Features

    The following table contains an overview of the electrical connection data for the Heraeus Megafuge 16/16R. This data is to be taken into consideration when selecting the mains connection socket. Table 1-4. Electrical connection data of the Heraeus Megafuge 16/16R. Mains...
  • Page 16: Rotor Selection

    Introduction and Description Rotor Selection Rotor Selection The Heraeus Megafuge 16/16R is supplied without a rotor. Various rotors are available to choose from. TX-400 7500 3629 with round buckets 7500 3655 TX-200 7500 3658 with round buckets 7500 3659 BIOShield...
  • Page 17 Before use Contents “Before Setting up” page 2-2 • • “Transporting the Centrifuge” page 2-2 “Location” page 2-2 • • “Aligning the Centrifuge” page 2-3 “Mains Connection” page 2-3 • • “Storage” page 2-4 Thermo Scientific Megafuge 16 / 16R...
  • Page 18: Chapter 2 Before Use

    People and hazardous substances must be kept out of the safety zone while centrifuging. • The supporting structure must be stable and free of resonance, for example a level laboratory bench. • The supporting structure must be suitable for horizontal setup of the centrifuge. Megafuge 16 / 16R Thermo Scientific...
  • Page 19: Aligning The Centrifuge

    3. Check whether the cable complies with the safety standards of your country. 4. Make sure that the voltage and frequency correspond to the figures on the rating plate. 5. Establish the connection to the power supply with the connecting cable. Thermo Scientific Megafuge 16 / 16R...
  • Page 20: Storage

    • Before storing the centrifuge and the accesories it must be cleanedand if necessary desinfected and decontaminated. • Store the centrifuge in a clean, dust-free location. • Be sure to place the centrifuge on its feet. • Avoid direct sunlight. Megafuge 16 / 16R Thermo Scientific...
  • Page 21: Control Panel

    Control Panel Contents “Control Panel” page 3-2 • • “Keys” page 3-2 Thermo Scientific Megafuge 16 / 16R...
  • Page 22: Keys

    Use the arrow keys in order to modify the displayed value Change Value Press the snow symbol key for prewarming or precooling the centrifuge. Snow Symbol Use the Change key to change the display mode. (Speed / RCF Value) Changing the display mode Megafuge 16 / 16R Thermo Scientific...
  • Page 23: Operation

    • • “Saving programs” page 4-6 “Centrifugation” page 4-7 • • “Temperature Adaptation during Standstill” page 4-8 “Short-term Centrifugation” page 4-8 • • “Removing the Rotor” page 4-8 “Aligning the Centrifuge” page 4-9 • Thermo Scientific Megafuge 16 / 16R...
  • Page 24: Switch On Centrifuge

    Note The lid should audibly click into place. Rotor Installation The approved rotors for the Heraeus Megafuge 16 / 16R are listed in section “Rotor Selection” page 1-6. Use only the rotors and accessories from this list in the centrifuge.
  • Page 25 4. If available close the rotor with the rotor lid. Be sure to check all sealings before starting any aeorsol-tight applications. See the information in the rotor instruction manual. 5. Close the centrifuge lid. Thermo Scientific Megafuge 16 / 16R...
  • Page 26: Entering Parameters

    Entering Parameters Entering Parameters The Heraeus Megafuge 16 / 16R offers you a total of 9 acceleration and 10 braking curves with which samples and gradients can be centrifuged. After the centrifuge is turned on, the last running profile selected is shown.
  • Page 27 1. Press the key below the TEMPERATURE display in order to open the temperature selection menu. The display shows "Set temp": 2. Enter the desired value by pressing the key repeatedly, until the desired value shows. Thermo Scientific Megafuge 16 / 16R...
  • Page 28: Saving Programs

    The display shows the following message: 2. Press the BUCKET key repeatedly until the bucket being used is displayed. Saving programs 1. Enter the program parameters. 2. Press any of the program store keys for 4 seconds. Megafuge 16 / 16R Thermo Scientific...
  • Page 29: Centrifugation

    The centrifuge will be decelerated at the designated rate. The message "END" will illuminate, and after pressing the key OPEN , the lid will open and you can remove the centrifuged material. Thermo Scientific Megafuge 16 / 16R...
  • Page 30: Temperature Adaptation During Standstill

    Short-term Centrifugation For short-term centrifuging, the Heraeus Megafuge 16 / 16R has a PULSE- function. By holding down the PULSE key , spinning will start and continue until the key is let go.
  • Page 31: Aerosol-Tight Rotors

    Figure 4-2. AutoLock -lid for aerosol-tight rotors WARNING Mind the AutoLock -mandrill inside the lid. Do not touch the mandrill. Aligning the Centrifuge • To turn off the centrifuge put the mains switch to "0". Thermo Scientific Megafuge 16 / 16R...
  • Page 32: Chapter 5 System Menu

    Language English Deutsch Francaise End of run beep? Español Italiano Nederlands к Росс ий Keypad beep? LCD powersave? Auto lid open? 0547V01 0547V01 Software ID: 4641V05 Software NVRAM identification example values. Cycle count: 2368 Thermo Scientific Megafuge 16 / 16R...
  • Page 33: Description

    Otherwise use the keys below the bucket selection until it says NO. Use the keys below the speed display in order to navigate through the system menu. Press the STOP key to quit the system menu. Megafuge 16 / 16R Thermo Scientific...
  • Page 34 Cycle count Here you fine the current numbers of cycles. Use the keys below the speed display in order to navigate through the system menu. Press the STOP key to quit the system menu. Thermo Scientific Megafuge 16 / 16R...
  • Page 35: Maintenance And Care

    Maintenance and Care Contents “Cleaning Intervalls” page 6-2 • • “Cleaning” page 6-2 “Disinfection” page 6-3 • • “Decontamination” page 6-4 “Autoclaving” page 6-5 • • “Service of Thermo Fisher Scientific” page 6-5 Thermo Scientific Megafuge 16 / 16R...
  • Page 36: Cleaning Intervalls

    • After cleaning, treat the entire surface of aluminum parts with corrosion protection oil (7000 9824). Also treat the cavities with oil. • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. Megafuge 16 / 16R Thermo Scientific...
  • Page 37: Disinfection

    CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific Megafuge 16 / 16R...
  • Page 38: Decontamination

    For general radioactive decontamination use a solution of equal parts of 70% ethanol, 10% SDS and water. 1. Open the centrifuge. 2. Turn off the centrifuge. 3. Pull out the power supply plug. Megafuge 16 / 16R Thermo Scientific...
  • Page 39: Autoclaving

    The service technicians check the following: • the electrical equipment • the suitability of the set-up site • the lid lock and the safety system • the rotor • the fixation of the rotor and the drive shaft Thermo Scientific Megafuge 16 / 16R...
  • Page 40 Thermo Fisher Scientific offers inspection and service contracts for this work. Any necessary repairs are performed for free during the warranty period and afterwards for a charge. This is only valid if the centrifuge has only been mainained by a Thermo Fisher Scientific service technician. Megafuge 16 / 16R Thermo Scientific...
  • Page 41: Trouble Shooting

    Trouble Shooting Contents “Mechanical Emergency Door Release” page 7-2 • • “Troubleshooting by User” page 7-3 “When to contact Customer Service” page 7-5 • Thermo Scientific Megafuge 16 / 16R...
  • Page 42: Mechanical Emergency Door Release

    4. Push the cord back into the centrifuge and mount the plug. Reconnect the centrifuge once the power has been restored. Switch on the centrifuge. Press the OPEN to have the door locks operative again. Megafuge 16 / 16R Thermo Scientific...
  • Page 43: Troubleshooting By User

    The lid opens while the device is running. The run does not start or the centrifuge Close the lid and restart the centrifuge. runs down without being braked. If an error message appears again, inform Customer Service. Thermo Scientific Megafuge 16 / 16R...
  • Page 44 Error during the self-test of the centrifuge program and the electronics. The run does not start or the centrifuge runs down without being braked. Restart the centrifuge. If an error message appears again, inform Customer Service. Megafuge 16 / 16R Thermo Scientific...
  • Page 45: When To Contact Customer Service

    1. Hold down any of the keys and then switch on the centrifuge. You enter the system menu. 2. Press the START key 3. Press the key, until the following message is displayed: Software ID: XXXXXXXX 4. Communicate the software version to the service technician. Thermo Scientific Megafuge 16 / 16R...
  • Page 46: Chemical Compatibility Chart

    S S U U S U M U S U U S U U U M U M U U U S U U S U S Benzene Benzyl Alcohol U U - M M - S U U U U U U U - M S M - Thermo Scientific Megafuge 16 / 16R...
  • Page 47 U S U - Ethylene Dichloride Ethylene Glycol S S S S S S S S S S S S - S U S S S S S S S S M S M S Megafuge 16 / 16R Thermo Scientific...
  • Page 48 S S S S S S Solution 555 (20%) Magnesium Chloride M S S - S S S S S S S S S S S S S S S S S S S M S S S Thermo Scientific Megafuge 16 / 16R...
  • Page 49 S U M U U S U M S M S U U S U M M M S Phenol (5%) Phenol (50%) U S U - S U M - S U M U U U U U S U M U U S U U U M S Megafuge 16 / 16R Thermo Scientific...
  • Page 50 S S S M S - Urea Hydrogen Peroxide (10%) U U M S S U U - S S S U S S S M U S S S S S S M S U S Thermo Scientific Megafuge 16 / 16R...
  • Page 51 No data available. Because no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. suggest testing, using sample to avoid loss of valuable material. Chemical resistance data is included only as a guide to product use. Megafuge 16 / 16R Thermo Scientific...
  • Page 52: Contact Information

    France +33 2 2803 2180 +33 2 2803 2000 Italy +39 02 02 95059 341 Netherlands +31 76 571 4440 Nordic / Baltic Countries +35 89 329 100 Russia +7 (812) 703 42 15 Thermo Scientific Megafuge 16 / 16R...
  • Page 53 +86 10 8419 3588 India +91 22 6716 2200 Japan +81 45 453 9220 Other Asian Countries +852 2885 4613 Latin America +1 866 984 3766 Other Countries +49 6184 90 6940 +33 2 2803 2180 Megafuge 16 / 16R Thermo Scientific...
  • Page 54 Failure message ..........7-3 Features ............1-5 Safety features ..........1-2 Safety zone ............2-2 Short-term Centrifugation .......4-8 Imbalance indicator ......... 4-7 Starting centrifuge program ......4-7 Intended Use ............v Stopping the centrifugation program ....4-7 Storage ............2-4 Thermo Scientific Megafuge 16 / 16R...
  • Page 55 Index: Switch on Centrifuge ........4-2 Technical Data ..........1-3 Temperature Adaptation .........4-8 Transporting the Centrifuge ......2-2 Trouble Shooting ..........7-1 Megafuge 16 / 16R Thermo Scientific...

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