Thermo Scientific 1500 Series Operating Instructions Manual

Thermo Scientific 1500 Series Operating Instructions Manual

Biological safety cabinet

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1500 Series B2
Class II, Type B2 
Biological Safety Cabinet
Operating Instructions
3848331 • Revision B • June 2019

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Summary of Contents for Thermo Scientific 1500 Series

  • Page 1 1500 Series B2 Class II, Type B2  Biological Safety Cabinet Operating Instructions 3848331 • Revision B • June 2019...
  • Page 2 IMPORTANT Read this instruction manual. Failure to follow the instructions in this manual can result in  damage to the unit, injury to operating personnel and poor equipment performance. CAUTION All internal adjustments and maintenance must be performed by qualified service personnel. Material in this manual is for informational purposes only.
  • Page 3: Table Of Contents

     Contents Preface ................ 1 Legal Information............1 Warranty ..............1 Introduction ..............2 Intended Use............2 Instruction of the Operating Personnel...... 2 Safety Precautions ............4 Prerequisites ..............6 Space Requirements..........6 Clearance Requirements .......... 6 Location Requirements ..........6 Exhaust System Requirements.........
  • Page 4 Connecting the Biosafety Cabinet to  Utility Service Lines ..........11 Using the SmartPort Cord & Cable Portal ..... 11 Installing the Drain Valve........... 12 Initial Certification ............. 12 Performance Features..........14 HEPA Filters ............. 14 ULPA Filters ............. 14 Laminar Airflow ............
  • Page 5 Appendix B: Dimensions ..........35 Appendix C: Specifications .......... 36 Environmental Conditions......... 36 Appendix D: Accessories ..........37 Telescoping Base Stands......... 37 Appendix E: Quick Chart..........39...
  • Page 6: Preface

    • The device is operated and serviced exclusively in accordance with its intended purpose and as described in these operating instructions • The device is not modified 1500 Series Class II, Type B2 Biological Safety Cabinet Preface |...
  • Page 7: Introduction

    This user’s manual To ensure optimum safety to the user and the environment, provides instructions on how to use the 1500 Series B2 most the biosafety cabinet should not be used in laboratories that effectively and safely.
  • Page 8 • To ensure safety during the repair of the cabinet, servicing of the equipment must be carried out by trained and authorized personnel. 1500 Series Class II, Type B2 Biological Safety Cabinet Introduction |...
  • Page 9: Safety Precautions

    If you suspect that a HEPA filter has occur if lifted or supported on the front edge. been damaged, DO NOT use the cabinet; contact a local certification agency or Thermo Fisher Scientific. Safety Precautions 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 10 Before performing certification tests requiring entry into contaminated areas.  Before relocating the cabinet.  Before changing research programs.  After the gross spill of biohazardous material or toxic chemicals. 1500 Series Class II, Type B2 Biological Safety Cabinet Safety Precautions |...
  • Page 11: Prerequisites

    For a further discussion of the cabinet’s exhaust system requirements, go to Getting Started. Prerequisites 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 12: Electrical Requirements

    Flammable gases or solvents may reach explosive concentrations in the cabinet or ductwork. If you feel that the procedure requires the use of an open flame or flammable materials, contact the institution’s safety office. 1500 Series Class II, Type B2 Biological Safety Cabinet Prerequisites |...
  • Page 13: Getting Started

    To access the nuts and bolts, remove the side panels by removing and keeping the two Phillips screws on both panels. Swing the front of each panel away from Getting Started 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 14: Moving And Lifting The Cabinet

    • Power Cord • SmartPort Accessories (optional) Base Stand Underneath the Thermo Scientific offers accessory Base Stands in a variety of work surface configurations to suit your particular needs. Stands can be ordered with adjustable telescoping legs or with a manually or electrically adjustable hydraulic lift.
  • Page 15: Exhaust System Connections

    If your research involves the use of toxic compounds or volatile materials, contact your facility’s safety officer or Thermo Fisher Scientific to ensure that your cabinet and its 10 | Getting Started 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 16: Exhaust System Requirements

    This will also 4. Push the tube into the fitting until it is properly seated. The tube will go approximately ¾" (19 mm) into the fitting. 1500 Series Class II, Type B2 Biological Safety Cabinet Getting Started |...
  • Page 17: Installing The Drain Valve

    6. Reinstall the work surface. 12 | Getting Started 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 18: Performance Features

    Performance Features All 1500 Series B2 Biosafety Cabinets operate using the following principles: Filter  Polyurethane seal frame Between media • Filtration and retention of particulates by High Efficiency pack and filter frame Particulate Air (HEPA) filter(s) • Laminar airflow •...
  • Page 19: Directional Airflow

    The airflow sensor reading is shown on the display as Inflow in feet per minute (FPM) or meters per second (M/S). Figure 11. Directional Airflow 14 | Performance Features 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 20: Cabinet Grilles, Ductwork And Air Balance Controls

    UV output of the lamp, the genus and species of the organism, the medium the agent is suspended in, etc. Contact the Health and Safety Officer at your facility for UV light use and recommendations. 1500 Series Class II, Type B2 Biological Safety Cabinet Performance Features |...
  • Page 21: Using The Cabinet

    Figure 16. The display will enter sleep mode, turning itself off, five minutes after the blower is turned off. 16 | Using the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 22: Alarm Screens

    Either the motor has failed, or the motor and display circuit board are not communicating properly. CAUTION: Do not use the cabinet until the problem has been corrected. 1500 Series Class II, Type B2 Biological Safety Cabinet Using the Cabinet |...
  • Page 23: Operating The Sliding Sash

    3. To turn the UV light on, the sash must be completely closed to prevent the escape of any UV radiation. Press [UV LIGHT] on the Keypad to activate the UV light. 18 | Using the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 24: Using The Cabinet Keypad

    To select from returns you to the previous menu level. the various menu options press the [UP] or [DOWN] buttons 1500 Series Class II, Type B2 Biological Safety Cabinet Using the Cabinet |...
  • Page 25 Chinese Japanese Startup Tone Language Clock SmartStart / Night Smart Figure 25. Configuration submenu Figure 27. Language selection 20 | Using the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 26 OFF is selected, the blower must be manually started from turn on when the sash is closed. When [OK/Mute] is pressed, the final configuration screen will appear. 1500 Series Class II, Type B2 Biological Safety Cabinet Using the Cabinet |...
  • Page 27 The security lock is deactivated by holding [DOWN] for three seconds. If blower is not turned on within 5 minutes of unlocking, the 22 | Using the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 28 It is password protected replace it. for safety. This screen displays the hours of UV lamp operation (Runtime), and how many hours remain (Remaining) until you 1500 Series Class II, Type B2 Biological Safety Cabinet Using the Cabinet |...
  • Page 29: Working In The Biosafety Cabinet

    • Check the air grilles for obstructions If An Airflow Alert Activates The most common causes of an Airflow Alert are: 24 | Using the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 30 Avoid the use of an open flame. Use disposable labware or an electric incinerator as alternatives • Use proper aseptic technique • Avoid using techniques or procedures that disrupt the airflow patterns of the cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet Using the Cabinet |...
  • Page 31: Maintaining The Cabinet

    1. Lift the front edge of the work surface straight up by grasping the knob handles at either front corner. Note: The grille must be thoroughly decontaminated before removing it. 26 | Maintaining the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 32 Also ensure that the Sampling/ Decontamination Tube (the black tube with an orange cap) rests behind the towel catch, allowing the catch to contact the rear wall of the cabinet. 1500 Series Class II, Type B2 Biological Safety Cabinet Maintaining the Cabinet |...
  • Page 33 7. When reinstalling the Left End Cap, the pins on each LED Lamp must align rotationally with the Left End Cap. This ensures the dead band stripe is positioned correctly for maximum cabinet lighting. 28 | Maintaining the Cabinet 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 34 1. Close the sash completely and seal the bottom edge with plastic sheeting. 2. Seal the exhaust outlet with plastic sheeting. 3. Unplug the cabinet. 1500 Series Class II, Type B2 Biological Safety Cabinet Maintaining the Cabinet |...
  • Page 35: Troubleshooting

    Lamp is defective Replace defective lamp Lamp wiring is disconnected Inspect lamp wiring Defective lamp ballast Replace lamp ballast Keypad disconnected or defective Run keypad diagnostics and check connections 30 | Troubleshooting 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 36 Cabinet is out of adjustment / HEPA Have cabinet recertified. If HEPA filters are filter(s) are defective defective or damaged, replace them 1500 Series Class II, Type B2 Biological Safety Cabinet Troubleshooting |...
  • Page 37: Appendix A: Components

    3838403 Supply HEPA Filter 6-ft 1297504 Lamp, LED, 4-ft Model 1297506 Lamp, LED, 6-ft Model 1271300 Lamp, UV (models with UV light only) 1287900 Receptacle Cover 32 | Appendix A: Components 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 38 Figure 49. Location of the service parts 1500 Series Class II, Type B2 Biological Safety Cabinet Appendix A: Components |...
  • Page 39: Appendix B: Dimensions

    Power Cord Connection 8.8" Power Cord  Connection 72.6" 61.7" 29.2" 21.7 Max Loading 21.7 Max Loading 25.7" 17.6" 31.2" Figure 50. Dimensions of the Biosafety Cabinet 34 | Appendix B: Dimensions 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 40: Appendix C: Specifications

    Used in an environment of Pollution degrees 2 (i.e., where normally only non-conductive atmospheres are present). Occasionally, however, a temporary conductivity caused by condensation must be expected, in accordance with IEC 664 1500 Series Class II, Type B2 Biological Safety Cabinet Appendix C: Specifications |...
  • Page 41: Appendix D: Accessories

    Catalog# For use with Shipping Weight 3858510 All 115 V AC models 5 lbs. (2.3 kg) 3858512 All 230 V AC models 5 lbs. (2.3 kg) 36 | Appendix D: Accessories 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 42 Rated at 99.999% efficiency with particles 0.12 microns. Table 11. ULPA filters Catalog# For use with 3859501 All 4-foot Thermo 1500 Series B2 BSC 3859503 All 6-foot Thermo 1500 Series B2 BSC 1500 Series Class II, Type B2 Biological Safety Cabinet Appendix D: Accessories |...
  • Page 43: Appendix E: Quick Chart

    4.03 Inflow Velocity Range 100-110 100-110 Supply HEPA Leak Test Data Air Displacement (CFM) Laskin Nozzles needed Theoretical aerosol conc. (ug/l) Actual aerosol conc. (ug/l) 38 | Appendix E: Quick Chart 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 44 3331104 3331105 Motor HP ½ ½ LED Lamp Data (2 each) Thermo P/N 1297505 1297506 Color (ºK) 4000 4000 Lumens 1850 3200 Glass Type Frosted Frosted 1500 Series Class II, Type B2 Biological Safety Cabinet Appendix E: Quick Chart |...
  • Page 45 7. Each motor must be programmed by Thermo Scientific for the appropriate width cabinet. The speed control settings will fluctuate depending on local temperature and pressure. 40 | Appendix E: Quick Chart 1500 Series Class II, Type B2 Biological Safety Cabinet...
  • Page 46 Evaluation and Restriction of Chemicals - Regulations and Directives  WEEE Compliance Great Britain WEEE Compliance. This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked with the following symbol. Thermo Fisher Scientific has contracted with one or more recycling/disposal companies in each EU Member State, and this product should be disposed of or recycled through them.
  • Page 47 We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service call.

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