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Thermo Scientific
BIOShield
Instruction Manual
50119946-f • 07 / 2020
Visit us online to register your warranty:
thermofisher.com/labwarranty
1000A
TM

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Summary of Contents for Thermo Scientific BIOShield 1000A

  • Page 1 Thermo Scientific BIOShield 1000A Instruction Manual 50119946-f • 07 / 2020 Visit us online to register your warranty: thermofisher.com/labwarranty...
  • Page 2 WEEE Conformity This product is subject to the regulations of the EU Waste Electrical & Electronic Equipment (WEEE) Directive 2012/19/EU. It is marked by the following symbol:...
  • Page 4 Issue Date: 15 January 2009 Test Summary A Thermo Scientific 75003603 contained rotor (Max speed 6,030 rpm) was supplied by Thermo Fisher and containment tested at 6,030 rpm using the method described i n Annex M of EN 61010-2-020. The rotor was shown to contain a spill when tested i n triplicate.
  • Page 5: Table Of Contents

    Checking the Aerosol-Tightness ................5-2 Chapter 6 Maintenance and Care ......................6-1 Cleaning intervalls ....................6-2 Cleaning ........................6-2 Disinfection ......................6-3 Decontamination .....................6-4 Autoclaving ......................6-5 Service of Thermo Fisher Scientific ................6-5 RCF-values ....................... A-1 Chemical Compatibility Chart ................B-1 Thermo Scientific 75003603 BIOShield 1000A...
  • Page 6 Contents 75003603 BIOShield 1000A Thermo Scientific...
  • Page 7: Preface

    • Do not use rotors which show any signs of corrosion and/or cracks. • Use only with rotors which have been loaded properly. • Never overload the rotor. • Never run the rotor without lid. Thermo Scientific 75003603 BIOShield 1000A...
  • Page 8 CAUTION means that material damage could occur. WARNING means that injuries or material damage or contamination could occur. This symbol refers to biological hazards. Observe the information contained in the instruction manual to keep yourself and your environment safe. 75003603 BIOShield 1000A Thermo Scientific...
  • Page 9: Rotor Specifications

    Rotor Specifications Contents • “Technical Data” page 1-2 Thermo Scientific 75003603 BIOShield 1000A...
  • Page 10: Technical Data

    17.8 / 8.2 17.8 / 8.2 Pitch angle [°] Accel. / braking time [s] 75 / 80 75 / 80 45 / 55 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003603 BIOShield 1000A Thermo Scientific...
  • Page 11 17.8 / 8.2 17.8 / 8.2 Pitch angle [°] Accel. / braking time [s] 115 / 80 115 / 80 120 / 75 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003603 BIOShield 1000A...
  • Page 12 17.8 / 8.2 17.8 / 8.2 Pitch angle [°] Accel. / braking time [s] 75 / 80 75 / 80 45 / 55 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003603 BIOShield 1000A Thermo Scientific...
  • Page 13 17.8 / 8.2 17.8 / 8.2 Pitch angle [°] Accel. / braking time [s] 115 / 80 115 / 80 120 / 75 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK Thermo Scientific 75003603 BIOShield 1000A...
  • Page 14 17.8 / 8.2 17.8 / 8.2 Pitch angle [°] Accel. / braking time [s] 115 / 90 115 / 90 100 / 75 Aerosol-tight Permissible temperature range autoclavable (cycle number) °C Tested by HPA, Porton-down, UK 75003603 BIOShield 1000A Thermo Scientific...
  • Page 15 Rotor Specifications Technical Data Table 1-6. Thermo Scientific 230 V, 50 / 60 Hz Centrifuge Thermo Scientific SL 40 Thermo Scientific SL 40 F Catalog # 75004512 75004542 Weight of empty rotor [kg] Max. cycle number 30,000 30,000 Maximum permissible load [g]...
  • Page 16 Rotor Specifications Technical Data Table 1-7. Thermo Scientific 120 V, 60 Hz Centrifuge Thermo Scientific SL 40 Catalog # 75004513 Weight of empty rotor [kg] Max. cycle number 30,000 Maximum permissible load [g] 4 x 250 Maximum speed n [rpm]...
  • Page 17: Accessories

    Accessories Contents • “Rotor data” page 2-1 “Accessories” page 2-1 • Thermo Scientific 75003603 BIOShield 1000A...
  • Page 18 Radius (cm) RCF/Radius 7,164 17.8 Maximum Minimum 3,300 BIOShield 1000A Rotor Package Cat. No. Description 75003603 BIOShield 1000A with buckets and sealing lid Tube Ordering Information Sealing Assembly Required: Adapters Required: Accessories Required: Tube Vol. Max. Dims ØxL Cat. No. Vol.
  • Page 19: Autolock Tm

    AutoLock Contents “Rotor Installation” page 3-2 • • “Removing the Rotor” page 3-3 Thermo Scientific 75003603 BIOShield 1000A...
  • Page 20: Rotor Installation

    WARNING If the rotor cannot be properly locked in place after several attempts, then the AutoLock is defective and you are not permitted to operate the rotor. Check for any damage to the rotor, damaged rotors must not be used. Keep the hub area clear of objects. 75003603 BIOShield 1000A Thermo Scientific...
  • Page 21: Removing The Rotor

    2. Grab the rotor handle with both hands and press against the green AutoLock button. At the same time, pull the rotor directly upwards with both hands and remove it from the centrifuge spindle. Make sure not to jam the rotor while doing this. Thermo Scientific 75003603 BIOShield 1000A...
  • Page 22 AutoLock Removing the Rotor 75003603 BIOShield 1000A Thermo Scientific...
  • Page 23 Rotor Loading Contents “Before a Run” page 4-2 • • “Proper Loading” page 4-2 “Improper Loading” page 4-3 • • “Maximum Loading” page 4-3 “Cycle Counter” page 4-4 • Thermo Scientific 75003603 BIOShield 1000A...
  • Page 24: Chapter 4 Rotor Loading

    Always be sure to use buckets of the same weight class, which is marked on the buckets themselves. Proper Loading To ensure safe operation of the centrifuge, the rotor must be evenly loaded at all times 75003603 BIOShield 1000A Thermo Scientific...
  • Page 25: Improper Loading

    • Reduce the fill level. • Reduce the speed. Use the table or the formula: Actual Load (g/bucket) 6300 6170 6048 5933 5824 5721 5623 5530 5442 5357 5277 5200 5126 5055 Thermo Scientific 75003603 BIOShield 1000A...
  • Page 26: Cycle Counter

    WARNING Replace the rotor when the specified number of cycles is reached. Due to the mechanical load, a rotor can break and thus damage the centrifuge Service life examples Usage profile Maximum service life at 30,000 cycles frequent use 7 years 20 runs / day 220 days / year 75003603 BIOShield 1000A Thermo Scientific...
  • Page 27: Aerosol-Tight Applications

    Aerosol-tight Applications Contents “Basic Principles” page 5-2 • • “Fill Level” page 5-2 “Checking the Aerosol-Tightness” page 5-2 • Thermo Scientific 75003603 BIOShield 1000A...
  • Page 28: Basic Principles

    The careful inspection of the seals and seal surfaces for signs of wear and damage such as cracks, scratches and embrittlement is extremely important. Aerosol-tight applications are not possible if the lids are open. Aerosol-tightness requires the correct operation when filling the sample vessels and closing the rotor lid. 75003603 BIOShield 1000A Thermo Scientific...
  • Page 29 Damaged or clouded rotor covers are to be replaced immediately! CAUTION This quick test is not suited for validating the aerosol tightness of your BIOShield 1000A. Check the seals and sealing surfaces of the lid thoroughly. Thermo Scientific 75003603 BIOShield 1000A...
  • Page 30 Aerosol-tight Applications Checking the Aerosol-Tightness 75003603 BIOShield 1000A Thermo Scientific...
  • Page 31: Maintenance And Care

    Maintenance and Care Contents “Cleaning intervalls” page 6-2 • • “Cleaning” page 6-2 • “Disinfection” page 6-3 “Decontamination” page 6-4 • • “Autoclaving” page 6-5 • “Service of Thermo Fisher Scientific” page 6-5 Thermo Scientific 75003603 BIOShield 1000A...
  • Page 32 • Store the aluminum parts at room temperature or in a cold-storage room with the cavities pointing down. CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. 75003603 BIOShield 1000A Thermo Scientific...
  • Page 33: Disinfection

    CAUTION Before using any cleaning or decontamination methods except those recommended by the manufacturer, users should check with the manufacturer that the proposed method will not damage the equipment. Observe the safety precautions and handling instructions for the cleaning agents used. Thermo Scientific 75003603 BIOShield 1000A...
  • Page 34: Decontamination

    For general radioactive decontamination, use a solution of equal parts of 70 % ethanol, 10 % SDS and water. Disinfect the rotor and accessories as follows: 1. Open the centrifuge. 2. Turn off the centrifuge. 75003603 BIOShield 1000A Thermo Scientific...
  • Page 35: Autoclaving

    • the suitability of the set-up site; • the lid lock and the safety system; • the rotor; • the fixation of the rotor and the drive shaft. Thermo Fisher Scientific offers inspection and service contracts for this work. Thermo Scientific 75003603 BIOShield 1000A...
  • Page 36 Maintenance and Care Service of Thermo Fisher Scientific 75003603 BIOShield 1000A Thermo Scientific...
  • Page 37: Rcf-Values

    17.8 1800 17.8 1900 17.8 2000 17.8 2100 17.8 2200 17.8 2300 17.8 1053 2400 17.8 1146 2500 17.8 1244 2600 17.8 1345 2700 17.8 1451 2800 17.8 1560 2900 17.8 1674 3000 17.8 1791 Thermo Scientific 75003603 BIOShield 1000A...
  • Page 38 17.8 2479 5381 5300 17.8 2575 5590 5400 17.8 2673 5803 5500 17.8 2773 6020 5600 17.8 2875 6241 5700 17.8 2979 6466 5800 17.8 3084 6694 5900 17.8 3191 6927 6000 17.8 3300 7164 75003603 BIOShield 1000A Thermo Scientific...
  • Page 39: Chemical Compatibility Chart

    S U U U U U U U - M S M - Boric Acid U S S M S S U S S S S S S S S S U S S S S S S S S S S Thermo Scientific 75003603 BIOShield 1000A...
  • Page 40 S S S U S U S S S U ® Ficoll-Hypaque M S S - S S S - S S S S - S S - S S S S S S S M S S S 75003603 BIOShield 1000A Thermo Scientific...
  • Page 41 S S S U S S M S S S S S U S U M S S S S S S S M S M U Methylene Chloride U U U U M S S U S U U S U U U U U M U U U S S M U S U Thermo Scientific 75003603 BIOShield 1000A...
  • Page 42 S S S S S S S S S S S S S S S S S S S Picric Acid S S U - S M S S S M S U S S S U S S S S U S U M S M S 75003603 BIOShield 1000A Thermo Scientific...
  • Page 43 S S S S S S S S S S S S S S S S S S S S S S S Citric Acid (10 %) M S S M S S M S S S S S S S S S M S S S S S S S S S S Thermo Scientific 75003603 BIOShield 1000A...
  • Page 44 Chemical resistance data is included only as a guide to product use. Because no organized chemical resistance data exists for materials under the stress of centrifugation, when in doubt we recommend pretesting sample lots. 75003603 BIOShield 1000A Thermo Scientific...
  • Page 45 Scope of Supply ..........iii Cycle Counter ..........4-4 Service Technician ..........6-5 Sorvall .............1-4 Decontamination ..........6-4 Disinfection ............ 6-3 Thermo Scientific ..........1-7 Technical Data..........1-2 Fill Level ............5-2 Improper Loading ........... 4-3 Maintenance ........... 6-1 Maximum Loading ......... 4-3...
  • Page 46 Thermo Electron LED GmbH Zweigniederlassung Osterode Am Kalkberg, 37520 Osterode am Harz Germany thermofisher.com/rotors © 2009-2020 Thermo Fisher Scientific Inc. All rights reserved. All trademarks are the property of Thermo Fisher Scientific Inc. and its subsidiaries unless otherwise indicated. Delrin, TEFLON, and Viton are registered trademarks of DuPont. Noryl is a registered trademark of SABIC. POLYCLEAR is a registered trademark of Hongye CO., Ltd.

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