Thermo Scientific 1400 Series Operating And Maintenance

Class ii, a2 biological safety cabinet
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Model 1400 Series
Class II, A2 Biological Safety Cabinet
Operating and Maintenance
Manual 7001440 Rev. 14

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Summary of Contents for Thermo Scientific 1400 Series

  • Page 1 Model 1400 Series Class II, A2 Biological Safety Cabinet Operating and Maintenance Manual 7001440 Rev. 14...
  • Page 2 Revised 1440-200-6 to rev 1 - reversed descriptions of Item 1 24253/HD-1535 5/08 Updated 1440-200-3 to rev 1 - increased clearance - 23023 to 730110 24399/HD-1530 3/08 Updated both assembly drawings (changed CL dimensions for exhaust transition) Thermo Scientific Model 1400 Series...
  • Page 3 This product is required to comply with the European Union’s Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the WEEE symbol. Thermo Scientific has contracted with one or more recycling/disposal companies in each EU Member State European Country, and this product should be disposed of or recycled through them.
  • Page 4 We can also provide you with a quotation on our Extended Warranty for your Thermo Scientific products. Whatever Thermo Scientific products you need or use, we will be happy to discuss your applications. If you are experiencing technical problems, working together, we will help you locate the problem and, chances are, correct it yourself...over the telephone without a service...
  • Page 6: Table Of Contents

    Start-Up Procedure ........6-2 Thermo Scientific Model 1400 Series...
  • Page 7 Warranty Information ........12-1 Model 1400 Series...
  • Page 8: Introduction

    All work must be performed beyond the intake grille, on the solid work tray. All buttons require only a quick press to activate, unless otherwise specified. Thermo Scientific Model 1400 Series...
  • Page 10: Installation

    Plug the line cord into the wall outlet. Warning Do not use a ground-fault-protected outlet/receptacle. Nuisance tripping could disrupt the protective air curtain supplied by this unit during operation. ▲ Thermo Scientific Model 1400 Series...
  • Page 11: Moving Through Doorways

    Section 2 Installation Moving Through Model 1400 Series Biological Safety Cabinets are designed to fit through a 34 inch doorway. Doorways To fit a Model 1440 (4 foot) or 1460 (6 foot) BSC through a narrow doorway, the unit can be tipped 10° from vertical, so that the front window is parallel to the doorway.
  • Page 12: Plumbing Connection

    2. Ensure the O-ring is seated on the back of the valve. 3. Fit the powder-coated valve into the hole from inside the cabinet. Valve cover Retaining ring Washer O-ring Gas valve Air/Vacuum valve - inside the cabinet Figure 2-3. Gas and Air/Vacuum Valve Installation Thermo Scientific Model 1400 Series...
  • Page 13 ▲ Figure 2-4. Push Down to Turn Gas Valve Figure 2-5. Shut-off Valve Note The screw head located behind the valve knob (Figure 2-5) is a built-in shut-off ball valve for service requirements. ▲ Model 1400 Series Thermo Scientific...
  • Page 14 This distance is required to compensate for the electric stand movement and allows the hose to move without creating stress on the side window glass. See Figure 2-6. ▲ Figure 2-6. Recommended Valve/Flexible Hose Connection Configuration Thermo Scientific Model 1400 Series...
  • Page 15: Exhaust Requirements

    The exhaust system should have safeguards against exhaust failure. A biological safety officer or other qualified individual is required to review agents and chemicals used inside the cabinet to determine if additional filtration treatment is necessary before venting to atmosphere. ▲ Model 1400 Series Thermo Scientific...
  • Page 16: Optional Exhaust Transition

    See Figure 2-11. Press the up arrow to raise the stand. Release when the desired position is reached. If the arrow is not released, movement stops when the stand reaches maximum height. The down arrow operates similarly. Thermo Scientific Model 1400 Series...
  • Page 17: Optional Ultra-Violet Light

    • Jacketed wire or cable 22 to 14 gauge UL rated 600V 80°C, the distance between the BSC and the facility exhaust control as the length. Approximately 3.5 feet is required inside the enclosure area. Model 1400 Series Thermo Scientific...
  • Page 18 4. Proceed with routing of cable to Rel. 1 on the board (Figure 2-13). The contact configuration is N.O. contacts close when the BSC fan is turned on. Common terminal Normally Open terminal Figure 2-13. Relay wiring Thermo Scientific Model 1400 Series...
  • Page 20: Certification Testing

    Biohazard Cabinet Field Certifier Program NSF International PO Box 130140 789 N. Dixboro Rd Ann Arbor, MI 48113-0140 Telephone (734) 769-8010 Or (800) NSF-MARK Fax (734) 769-0109 http://www.nsf.org/Certified/Biohazard-Certifier Thermo Scientific Model 1400 Series...
  • Page 21 Technical Services Department for additional references. Note Unless certification was expressly called for in the specification, quotes and/or purchase order, the cost for this on-site testing is to be paid for by the customer. ▲ Model 1400 Series Thermo Scientific...
  • Page 22 3.351 sq. ft. Direct Reading Instrument Method Inflow volume (exhaust volume) cu. ft. ÷ Front access opening 3.351 sq. ft. = Calculated face velocity LFPM Acceptable range for face velocity 100-110 LFPM TESTED BY: DATE: Thermo Scientific Model 1400 Series...
  • Page 23 2.681 sq. ft. Direct Reading Instrument Method Inflow volume (exhaust volume) cu. ft. ÷ Front access opening 2.681 sq. ft. = Calculated face velocity LFPM Acceptable range for face velocity 100-110 LFPM TESTED BY: DATE: Model 1400 Series Thermo Scientific...
  • Page 24 4.925 sq. ft. Direct Reading Instrument Method Inflow volume (exhaust volume) cu. ft. ÷ Front access opening 4.925 sq. ft. = Calculated face velocity LFPM Acceptable range for face velocity 100-110 LFPM TESTED BY: DATE: Thermo Scientific Model 1400 Series...
  • Page 25 3.940 sq. ft. Direct Reading Instrument Method Inflow volume (exhaust volume) cu. ft. ÷ Front access opening 3.940 sq. ft. = Calculated face velocity LFPM Acceptable range for face velocity 100-110 LFPM TESTED BY: DATE: Model 1400 Series Thermo Scientific...
  • Page 26: Constricted Opening Inflow Test Method

    1.33 k factor = adjusted inflow lfpm Acceptable range for face velocity: 100 - 110 lfpm Exit Service Mode by pressing the same sequence of buttons as entering this mode. The face icons reappear above the Performance Monitor. Thermo Scientific Model 1400 Series...
  • Page 27 1.285 k factor = adjusted inflow lfpm Acceptable range for face velocity: 100 - 110 lfpm Exit Service Mode by pressing the same sequence of buttons as entering this mode. The face icons reappear above the Performance Monitor. Model 1400 Series Thermo Scientific...
  • Page 28 1.358 k factor = adjusted inflow lfpm Acceptable range for face velocity: 100 - 110 lfpm Exit Service Mode by pressing the same sequence of buttons as entering this mode. The face icons reappear above the Performance Monitor. Thermo Scientific Model 1400 Series...
  • Page 29 1.362 k factor = adjusted inflow lfpm Acceptable range for face velocity: 100 - 110 lfpm Exit Service Mode by pressing the same sequence of buttons as entering this mode. The face icons appear above the Performance Monitor. 3-10 Model 1400 Series Thermo Scientific...
  • Page 30: Operation

    The performance monitor graph should be fully lighted, if the filter is clean and the fan unobstructed. An automatic start-up timer is available. Instructions follow later in this section. Thermo Scientific Model 1400 Series...
  • Page 31 Hour counter or clock Unsafe window position Day of the week indicator* (icon flashes, alarm sounds, UV timer Auto fan start-up red background flashes) active timer active Figure 4-2. LCD Display 1=Monday, 7=Sunday Model 1400 Series Thermo Scientific...
  • Page 32: Set Time And Date

    (January = 01, February = 02, etc.), press Enter. Figure 4-4. Flashing Month 4. Set the date using the up/down arrow keys. When the correct date is displayed, press Enter. Figure 4-5. Flashing Date Thermo Scientific Model 1400 Series...
  • Page 33 Figure 4-8. Flashing Minutes 8. Set automatic Daylight Savings Time mode using the up/down arrow keys. When the correct mode (No Correction-NO, USA-US, Australian-AU, European-EU) is displayed, press Enter. Figure 4-9. Flashing Automatic Daylight Saving Time Area Model 1400 Series Thermo Scientific...
  • Page 34: Set Automatic Fan Start-Up Timer

    2. The minute digits flash. Set the minutes (1 to 59 minutes) by using the up/down arrows keys. When the minutes are selected, press Enter. 3. The UV light and clock icon appear left of the display when the UV timer is set. Thermo Scientific Model 1400 Series...
  • Page 35: Front Window

    Gently push the instrument to remove the plug. The hole must be sealed again prior to using the cabinet. Caution Edges of the exposed hole may be sharp after plug is removed. ▲ Model 1400 Series Thermo Scientific...
  • Page 36: Fan Hours Monitoring

    (if enabled). This alarm indicates that the cabinet may not be operating in a safe condition and requires service to correct the problem. Potential causes of this alarm are a clogged supply filter or an obstructed downflow sensor. Thermo Scientific Model 1400 Series...
  • Page 37 (if enabled). This alarm indicates that the cabinet may not be operating in a safe condition and requires service to correct the problem. Potential causes for this alarm are clogged supply and exhaust filters, or a fan motor bearing failure. Model 1400 Series Thermo Scientific...
  • Page 38: General Cautions

    Damage could occur. ▲ Caution Avoid causing damage to the downflow sensor when cleaning around it. The sensor is located on the right side of the chamber just below the supply filter. ▲ Thermo Scientific Model 1400 Series...
  • Page 40: Cabinet Start-Up

    Cabinet Checklist 1. Verify that the Drain valve is closed (the handle turned horizontal). 2. Verify that all service valves are closed. 3. Verify that the cardboard exhaust filter protector has been removed. Thermo Scientific Model 1400 Series...
  • Page 41: Start-Up Procedure

    4. Turn the fan on. 5. Place everything needed into the cabinet. 6. Place the window at the working height of 10 inches for Models 1440 and 1460, and 8 inches for Models 1448 and 1468. Model 1400 Series Thermo Scientific...
  • Page 42: Specifications

    2 Pollution Degree describes the amount of conductive pollution present in the operating environment. Pollution Degree 2 assumes that normally only non-conductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation. Thermo Scientific Model 1400 Series...
  • Page 43 NEMA 5-20P NEMA 5-20P GFCI protected duplex GFCI protected duplex Outlets (2) 120V (7 Amps total) 120V (7 Amps total) * Add 30% to CFM values if using a thimble type exhaust transition with the cabinet. Model 1400 Series Thermo Scientific...
  • Page 44 Section 7 Specifications Thermo Scientific Model 1400 Series...
  • Page 45 Section 7 Specifications Model 1400 Series Thermo Scientific...
  • Page 46: Unit Attachment Locations

    Section 7 Specifications Unit Attachment Locations Thermo Scientific Model 1400 Series...
  • Page 47 Section 7 Specifications Unit Attachment Locations (continued) Model 1400 Series Thermo Scientific...
  • Page 48: Unit Center Of Gravity Locations

    Section 7 Specifications Unit Center of Gravity Locations Thermo Scientific Model 1400 Series...
  • Page 49 Section 7 Specifications Unit Center of Gravity Locations (continued) Model 1400 Series Thermo Scientific...
  • Page 50: Troubleshooting

    Low voltage is being applied to the cabinet. • Blower motor is defective. • Supply HEPA filter and Exhaust HEPA filter may be loaded. Decontaminate the unit and replace both HEPA filters. • Window is below normal working height. Thermo Scientific Model 1400 Series...
  • Page 51 UV light harness connection. Check connection at micro board J4, pins 3 and 4. • Defective UV lamp - Replace lamp. • Defective starter - Replace starter. • Defective UV ballast - Replace UV ballast (located behind lamp bulb). Model 1400 Series Thermo Scientific...
  • Page 52 Problem 4: Loud screeching noise Possible causes: • Bearings are bad in the motor blower assembly. • Blower wheel is rubbing against the housing. Problem 5: No power at GFI outlet Possible causes: • Switch requires reset Thermo Scientific Model 1400 Series...
  • Page 54: Routine Maintenance

    5. Carefully raise the front window to the highest position with the up arrow key. Lift the front cover. Clean the window on the inside. Close the cover. 6. Put the clean work surfaces back in place. Allow the fan to run for 10 minutes. Thermo Scientific Model 1400 Series...
  • Page 55: Main Filter Replacement

    7. Remove the filter. The filter gasket may stick to the cabinet frame. To facilitate removal, insert a smooth-edged flexible instrument between cabinet frame and filter. Be careful not to damage the frame. 8. Install the new filter. Apply soapy water to the gasket to ease installation. Model 1400 Series Thermo Scientific...
  • Page 56: Exhaust Filter Replacement

    7. Lower the frame with the filter. Remove the filter without damaging the gasket. 8. Install the new filter. 9. Re-install the frame and filter. 10. Check that the filter is centered in the filter opening, with equal portions of gasket showing on all sides. Thermo Scientific Model 1400 Series...
  • Page 57: Access To Electrical Parts

    To gain access to the components, remove the screws from the top of the cabinet (Figure 9-4). Set the protective cover aside. Figure 9-4. Remove Screws Model 1400 Series Thermo Scientific...
  • Page 58: Fuses

    Tilt the PCB board into an upright position until the board bracket sits unaided, held in the two side clamps. Figure 9-5. PCB Plate Upright Fuses on the control panel (PCB) for Model 1400 Series: Table 9-1. Fuses and Descriptions Component...
  • Page 59: Pcb Board Replacement

    6. Remove the hooks from both sides. 7. Lift the plenum chamber from the brackets on both sides so that the plenum is free of the filter. Then locate the brackets onto the safety hook at the sides. See Figure 9-2. Model 1400 Series Thermo Scientific...
  • Page 60 13. Place the plenum on a table, where the motor/fan can be easily replaced. 14. The new motor and plenum are reinstalled in the cabinet in reverse order. Thermo Scientific Model 1400 Series...
  • Page 61: Ultra-Violet Light Bulb Replacement

    5. Replace with new lamp. Rotate 1/4 turn to lock in position. 6. The UV system is installed on the panel. Notice the thumbscrew located on the end. See Figure 9-8. Figure 9-7. UV System on Panel Figure 9-8. Thumbscrew Model 1400 Series Thermo Scientific...
  • Page 62: Application And Use Of Uv Light

    Usage of the UV light can leave a thin film coating on the lamp, reducing the efficiency of the radiation. Use alcohol and a soft cloth to remove. The UV lamp has 5000 operating hours life, but efficiency is reduced before the operating life is reached. Thermo Scientific Model 1400 Series...
  • Page 63: Cleaning/Removal Of Divided Work Tray

    Clean the tray. 4. When cleaning is completed, reinstall the work tray on the trough. Lift the tray up slightly when pushing into position to avoid hitting the frame with the edge of the tray. 9-10 Model 1400 Series Thermo Scientific...
  • Page 64: Vertical Adjustment Of The Stand

    4. Place a pallet between the trough and the pallet truck to avoid scratches. 5. Lift the working bench with the pallet lift or pallet truck just until the cabinet feet clear the ground. The bench must be supported safely, without rocking or tipping. Thermo Scientific Model 1400 Series 9-11...
  • Page 65 Figure 9-13. Two Screws Under Trough Figure 9-14. Top Nuts 8. Remove the stand. 9. Loosen the three screws as shown in Figure 9-15. Be prepared for the weight as the plate is disconnected. Figure 9-15. Remove Adjustment Plates 9-12 Model 1400 Series Thermo Scientific...
  • Page 66 11. When installing the stand sides, the two top studs must be located into the holes. 12. Install the two screws under the trough in both sides of the stand. 13. Reinstall the rear plate. 14. Follow leveling procedure in Section 2. Thermo Scientific Model 1400 Series 9-13...
  • Page 67 Never use abrasive cleaners, scouring pads or steel wool. If the painted surfaces do become dull, streaked, smeared or marred in some other way, there is no known method to restore the finish. 9-14 Model 1400 Series Thermo Scientific...
  • Page 68: Service

    {maximum air flow}): "1 2 3 4 5 [6] 7" weekday indication • Raw inflow sensor output (range of 0 {no air flow} to 255 {maximum air flow}): "1 2 3 4 5 6 [7]" weekday indication Figure 10-1. Service Mode Display Thermo Scientific Model 1400 Series 10-1...
  • Page 69 Set Air Flow Alarms Reset Filter Time Counter Silence Options (en/disable) Enable/disable/delay Audible Alarm Enter Ring-back Time Enable/Disable UV Reset Processor Default Function * * Cabinet will revert to software default setpoints. Cabinet will require re-certification. 10-2 Model 1400 Series Thermo Scientific...
  • Page 70: Fan Cfm Manual Adjustment

    Program, Normal Fan, Silence and Down keys. Set each of the three alarms, using the up/down and Enter keys, to the following values: dL = 0, dH = 255, mL = 0. This prevents the airflow alarms from sounding during the setting process. Thermo Scientific Model 1400 Series 10-3...
  • Page 71 7" indicated at the bottom of the display), subtract 3 from this reading and record as Downflow Sensor Reading in the +20% Downflow row of the worksheet. This is the value of the Downflow Low alarm setting (dH). 10-4 Model 1400 Series Thermo Scientific...
  • Page 72 3. If the DFLO alarm does not sound within 15 seconds, enter Alarm Setting mode and increase the value displayed by one or two counts. Repeat Steps #1 through 3 until the DFLO alarm sounds within 15 seconds. Thermo Scientific Model 1400 Series 10-5...
  • Page 73 CFM Adjustment mode. The airflow alarm setup procedure is now complete. Table 10-3. Airflow Alarm Worksheet Total CFM Adjustment Downflow Sensor Alarm Condition Downflow Actual Inflow Actual Inflow Sensor Output Setting Output Nominal -20% Downflow (dL) +20% Downflow (dH) -20% Inflow (mL) 10-6 Model 1400 Series Thermo Scientific...
  • Page 74: Performance Monitor Reset

    UV light option is not installed. Simultaneously press the Program, Silence and down buttons to enable ('Uv: on' on display) or disable ('Uv: oF' on the display) the UV Light function. Thermo Scientific Model 1400 Series 10-7...
  • Page 75: Processor Reset

    Exhaust flow sensor calibration: zero offset Downflow Low alarm: 110 Downflow High alarm: 200 Exhaust Flow Low alarm: 100 UV light: enabled Audible Alarm mode: Silence enabled, beep with delay enabled Ring-back delay: 5 minutes 10-8 Model 1400 Series Thermo Scientific...
  • Page 76: Diagnostic Features

    12V OK: Lit if the +12V power supply is working properly • 14V OK: Lit if the +14V power supply is working properly • 17V OK: Lit if the +17V power supply is working properly • uC OK: Blinking if microcontroller is operating properly Thermo Scientific Model 1400 Series 10-9...
  • Page 77: Rs232 Diagnostic Port

    Line 4: Fan a1, Fan a2, Fan a3H, Fan a3L, Fan a4 (CR)(LF) o Fan Constants: XXh (two upper case hex characters followed by a lower case h indicating HEX data) 10-10 Model 1400 Series Thermo Scientific...
  • Page 78: Periodic Data String

    Status type as indicated by digit position starting from the left: • Spare • Spare • Stall • Cutback • Run • Start • Ramp • Trip Thermo Scientific Model 1400 Series 10-11...
  • Page 79: Parts List

    Fuse 6.25A Time Delay 1/4 x 1-1/4 230224 Fuse 6.25A Time Delay 1/4 x 1-1/4 230110 Fuse 1A Time Delay 1/4 x 1-1/4 230110 Fuse 1A Time Delay 1/4 x 1-1/4 285771 15W Fluorescent Starter 500009 Ballast F30T8 118V/60Hz 10-12 Model 1400 Series Thermo Scientific...
  • Page 80 Section 10 Service Thermo Scientific Model 1400 Series 10-13...
  • Page 81 Section 10 Service 10-14 Model 1400 Series Thermo Scientific...
  • Page 82 Section 10 Service Thermo Scientific Model 1400 Series 10-15...
  • Page 83 Section 10 Service 10-16 Model 1400 Series Thermo Scientific...
  • Page 84 Section 10 Service Thermo Scientific Model 1400 Series 10-17...
  • Page 85 Section 10 Service 10-18 Model 1400 Series Thermo Scientific...
  • Page 86: Accessories

    Section 10 Service Accessories Models 4-foot 6-foot Forma Model 1400 Series, Class II, A2, BSC 115V, 60 Hz with 8-inch sash opening 1448 1468 Forma Model 1400 Series, Class II, A2, BSC 115V, 60 Hz with 10-inch sash opening 1440 1460...
  • Page 88: Electrical Schematics

    Section 11 Electrical Schematics Thermo Scientific Model 1400 Series 11-1...
  • Page 89 Section 11 Electrical Schematics 11-2 Model 1400 Series Thermo Scientific...
  • Page 90 Section 11 Electrical Schematics Thermo Scientific Model 1400 Series 11-3...
  • Page 92: Warranty Information

    Section 12 Warranty Information Thermo Scientific Model 1400 Series 12-1...
  • Page 93 Section 12 Warranty Information 12-2 Model 1400 Series Thermo Scientific...
  • Page 94 For your convenience we have included a partial list of agencies that perform certification on our website. If you do not find someone listed in your area, please contact Thermo’s Technical Services department for additional references. Thermo Scientific Model 1400 Series...
  • Page 96 Thermo Scientific Controlled Environment Equipment 401 Millcreek Road, Box 649 Marietta, Ohio 45750 USA www.thermo.com...

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