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- Page 1 1300 Series B2 Class II, Type B2 Biological Safety Cabinet Operating Instructions 3848304 Rev. 0...
- Page 2 Copyright These operating instructions are protected by copyright. Rights resulting thereof, particularly reprint, photomechanical or digital postprocessing or reproduction, even in part, are only allowed with the written consent of the Thermo Fisher Scientific Inc. This regulation does not apply to reproductions for in-plant use. Trademarks All trademarks mentioned in the operating instructions are the exclusive property of the respective manufacturers.
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Page 3: Table Of Contents
Operating Instructions Safety Cabinet | 1300 Series B2 Contents CHAPTER 1: INTRODUCTION ................1 Instruction of the Operating Personnel ..............2 Warranty....................... 2 CHAPTER 2: PREREQUISITES................3 Space Requirements .................... 3 Suggested Location....................4 Exhaust Requirements ..................5 Electrical Requirements..................5 Service Line Requirements .................. - Page 4 Operating Instructions Safety Cabinet | 1300 Series B2 Contents CHAPTER 6: MAINTAINING THE CABINET.............40 Routine Maintenance Schedule ................40 Service Operations .....................40 Work Surface Removal ................40 Front Grille Removal ..................40 Towel Catch Removal..................41 Front Panel Removal ...................42 Changing the B2 Prefilter................43 Changing the Fluorescent Lamps ..............43 Changing the Optional UV Lamp ..............43 Resetting a Circuit Breaker ................44...
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Page 5: Chapter 1: Introduction
Introduction Dear User, Congratulations on your purchase of a Thermo Scientific 1300 Series B2 Class II, Type B2 biosafety cabinet. Your 1300 Series B2 biosafety cabinet has been tested and certified in accordance to NSF/ANSI 49, and is designed to protect the user, the environment, and your research from harmful substances and cross-contamination. -
Page 6: Instruction Of The Operating Personnel
The operating instructions describe the safety cabinet 1300 Series B2, and applies to the models: • 4-foot models 1310 / 1311 / 1315 / 1316 / 1317 / 1318 • 6-foot models 1360 / 1361 / 1362 / 1363 / 1364 / 1365... -
Page 7: Chapter 2: Prerequisites
Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Before you install the Biosafety Cabinet, you need to prepare the site for installation. Carefully examine the location where you intend to install the cabinet. You must be certain that the area is level and of solid construction. In addition, a dedicated source of electrical power must be located near the installation site. -
Page 8: Suggested Location
Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Suggested Location Note: The Biosafety Cabinet should be located away from traffic patterns, doors, fans, ventilation registers, fume hoods and any other air-handling devices that could disrupt its airflow patterns. All windows in the room should remain closed. -
Page 9: Exhaust Requirements
The different Biosafety Cabinet models have the following electrical requirements: Table 2-1 Model # Requirements 1310, 1311 115 VAC, 60 Hz, 12 Amps 1315, 1316, 1317, 1318 230 VAC, 50/60 Hz, 6 Amps 1360, 1361 115 VAC, 60 Hz, 12 Amps... -
Page 10: Service Line Requirements
Operating Instructions Safety Cabinet | 1300 Series B2 Prerequisites Service Line Requirements All utility service lines should be ¼ inch O.D., brass, copper, or stainless steel, and equipped with an easily accessible shut-off valve. If the service line pressure exceeds 100 PSI, it must be equipped with a pressure regulator to reduce the line pressure. -
Page 11: Chapter 3: Getting Started
Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Now that the installation is properly prepared, you are ready to inspect, install, and certify the Biosafety Cabinet. This chapter covers how to: • Unpack and move the Biosafety Cabinet. •... -
Page 12: Unpacking The Biosafety Cabinet
Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Unpacking the Biosafety Cabinet Carefully remove the outer carton and inspect the cabinet for damage that may have occurred in transit. If the Biosafety Cabinet is damaged, notify the delivery carrier immediately and retain the entire shipment intact for inspection by the carrier. -
Page 13: Installing The Biosafety Cabinet On A Base Stand
Getting Started Installing the Biosafety Cabinet on a Base Stand Thermo Scientific offers accessory Base Stands that effectively support the weight of the cabinet. Stands for a B2 cabinet generally are not mobile, since it is connected to an exhaust duct. -
Page 14: Connecting The Biosafety Cabinet To Utility Service Lines
Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started CAUTION: Only lift and support the cabinet at the stainless steel sides and back. Damage will occur if lifted or supported on the front edge. Move the base stand into its final location. Using a carpenter’s level, adjust each leveling foot until the stand is level in both planes as shown in Figure 3-2. -
Page 15: Exhaust System Connections
Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Tighten the tube fitting nut hand tight and then, using a 7/16-inch wrench, tighten it at least ¾ turn more. Close the service valve in the Biosafety Cabinet and then slowly open the shutoff valve on the service valve. -
Page 16: Exhaust System Requirements
Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Exhaust System Requirements The cabinet exhaust stack is sized to accept 10-inch (254 mm) stainless steel ductwork. The exhaust system’s blower should be sized to handle the exhaust volume of each cabinet, as shown in the QuickCharts, Appendix E. The exhaust system must maintain a static pressure equal to or greater than the system pressure, plus 1.5 inches (38 mm) of water for the 1300 Series B2 cabinet. -
Page 17: Initial Certification
Operating Instructions Safety Cabinet | 1300 Series B2 Getting Started Figure 3-4 NOTE: If desired, the drain valve assembly attaches to the underside of the cabinet bottom. Apply a light coat of silicone sealant to this surface of the connector, aligning the three holes in the connector with the three holes in the Biosafety Cabinet liner. - Page 18 *These tests are user comfort related tests and may be omitted at the user’s or certifier’s discretion. If you have any questions regarding certification agencies or need assistance in locating one, contact Thermo Scientific at 1-888-213-1790. NOTE! Service with costs:...
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Page 19: Chapter 4: Performance Features And Safety Precautions
The number of particles that penetrate the filter are quantified, and this establishes the efficiency of the filter. Thus, the filters used in the Thermo Scientific 1300 Series B2 Cabinets are at least 99.99% efficient in removing particles 0.3 micron. -
Page 20: Figure 4-1 Hepa Filters
Operating Instructions Safety Cabinet | 1300 Series B2 Performance Features and Safety Precautions Figure 4-1 Filter frame Polyurethane seal between media pack and filter frame Filter media pack – A continuous sheet of filter media... -
Page 21: Laminar Airflow
Operating Instructions Safety Cabinet | 1300 Series B2 Performance Features and Safety Precautions Laminar Airflow Laminar airflow is defined as the movement of a body of air in a single direction, with a uniform velocity. In practice, the laminar downflow of air in the cabinet captures any aerosol generated in the work area of the cabinet, and directs it to the HEPA filters. -
Page 22: Directional Airflow
Operating Instructions Safety Cabinet | 1300 Series B2 Performance Features and Safety Precautions Directional Airflow Directional airflow also plays a key role in biosafety cabinet performance. Air is drawn into the front of the cabinet at the front grille. This “curtain” of air makes it more difficult for aerosols to escape out of the work area of the cabinet and into the outside environment. -
Page 23: Motor/Blower
The external exhaust pulls the airflow into the front of the cabinet and from the supply filter through the exhaust filter and out of the building. The motor in the Thermo Scientific 1300 Series B2 Cabinet is an electronically commutated motor (ECM). The ECM is a brushless DC motor that includes its own power supply to convert the incoming alternating current to direct current, as well as its own microprocessor to control and measure the motor’s operation. -
Page 24: Airflow Sensor
Operating Instructions Safety Cabinet | 1300 Series B2 Performance Features and Safety Precautions Airflow Sensor An airflow sensor, located above the Exhaust HEPA filter, constantly monitors the flow of exhaust air out of the cabinet. If the exhaust falls below a safe level, the control board turns off the cabinet blower, and sounds an audible and visual alarm. -
Page 25: Safety Precautions
Operating Instructions Safety Cabinet | 1300 Series B2 Performance Features and Safety Precautions Safety Precautions WARNING: The Biosafety Cabinet should be certified by a certification technician before its initial use. The cabinet should be recertified whenever it is relocated, serviced or at least annually thereafter. Do not assume that filter integrity and airflow performance have not been compromised during shipment. - Page 26 Operating Instructions Safety Cabinet | 1300 Series B2 Performance Features and Safety Precautions When surface disinfecting the Biosafety Cabinet: • Avoid splashing the disinfecting solution on skin or clothing. • Ensure adequate ventilation. • Carefully follow the disinfectant’s safety instructions. •...
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Page 27: Chapter 5: Using The Cabinet
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Correct Use The biosafety cabinet is a laboratory device for installation and operation in microbiological and biotechnical laboratories of safety levels 1, 2, and 3. It has been designed as a Class II safety cabinet, Type B2, in accordance with NSF/ANSI, Standard 49/2004. -
Page 28: System Reset Switch
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet System Reset Switch The Biosafety Cabinet has a system reset switch for resetting its microprocessors. The switch is located on the front of the electronics module, on top of the Cabinet, as shown in Figure 5-1. Ensure that the switch is in the “ON” (up) position before attempting to operate the Cabinet. -
Page 29: Status Area
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Status Area The Status Area displays the system’s condition during operation. This line can display any of the following messages: The motor is operating properly, and the sash is not open too far. Sash is too high The sash is open too far for safe operation. -
Page 30: Operating The Sliding Sash
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Fluorescent light In this mode the fluorescent lights are operating. When the sash is fully closed, the fluorescent lights automatically turn off. UV light In this mode the UV (germicidal) light is operating. When the sash is fully closed, the UV light can be programmed to automatically turn on. -
Page 31: The Control Keypad
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet To turn the UV light on, the sash must be completely closed to prevent the escape of any UV radiation. Push the UV light button to activate the UV light. Note: The sash must be completely closed for the UV light to activate. -
Page 32: Figure 5-4 Programming Menu Instructions
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Figure 5-4... - Page 33 Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet...
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Page 36: User Selectable Features
Using the Cabinet User Selectable Features The Thermo Scientific 1300 Series B2 Biosafety Cabinet offers the user unparalleled flexibility and convenience. The operation of the blower, fluorescent and UV lights can either be operated manually, or you may choose to use the Auto Start feature that activate functions automatically when the sash is opened or closed. -
Page 37: Interval Timer Operation
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Interval Timer Operation When selected, the Interval Timer menu is displayed on the LCD. The timer defaults to 0:00:00 (hours:minutes:seconds). Press and hold the “▲” or “▼” buttons to increase or decrease the timer interval. -
Page 38: Uv Lamp Timer
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet UV Lamp Timer The UV Lamp Timer allows you to decide whether the UV lamp will operate continuously or for a time period that you select. Interval When the “Operating Time” option is selected, the UV Operating Time menu is displayed. -
Page 39: Light Maintenance
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Manual When the “manual touchpad” option is selected, the fluorescent lights must be turned on and off at the touchpad. Light Maintenance This selection allows you to check how many hours the fluorescent lamps have burned. - Page 40 Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet The security lock feature prevents operation of the Biosafety Cabinet by unauthorized users. To engage the security lock, access the Security Lock submenu, as described in Figure 5-4. Select the “secure” option, and press the “OK”...
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Page 41: If An Exhaust Error Activates
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Diagnostic Test Operation When the “Diagnostic Test” menu is selected, the LCD allows keypad/ hardware testing to help troubleshoot any malfunction. Keypad Testing When keypad testing is selected, the LCD shows when each key is pressed, to confirm the touchpad is working. -
Page 42: Working In The Biosafety Cabinet
Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet Working In the Biosafety Cabinet Planning • Thoroughly understand procedures and equipment required before beginning work. • Arrange for minimal disruptions, such as room traffic or entry into the room while the cabinet is in use. - Page 43 Operating Instructions Safety Cabinet | 1300 Series B2 Using the Cabinet • Use proper aseptic technique. • Avoid using techniques or procedures that disrupt the airflow patterns of the cabinet. • If there is a spill or splatter during use, all objects in the cabinet should be surface decontaminated before removal.
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Page 44: Chapter 6: Maintaining The Cabinet
Operating Instructions Safety Cabinet | 1300 Series B2 Maintaining the Cabinet The common service operations necessary to maintain the Biosafety Cabinet for peak performance are listed below. Routine Maintenance Schedule Weekly • Using 70% ethanol, or a suitable disinfectant, surface disinfect the inside of the cabinet and the work surface. -
Page 45: Towel Catch Removal
Operating Instructions Safety Cabinet | 1300 Series B2 Maintaining the Cabinet Pull the other end of the sash up and away from the bottom edge of the cabinet. Reinstall the grille by reversing the above sequence, ensuring that the grille properly engages the bottom edge of the cabinet. Figure 6-1 Towel Catch Removal: Although not normally required, the towel catch can be removed for cleaning,... -
Page 46: Front Panel Removal
Operating Instructions Safety Cabinet | 1300 Series B2 Maintaining the Cabinet Figure 6-2 Front Panel Removal: Figure 6-3 Locate and remove the two Phillips screws that secure the front panel as shown in Figure 6-3. They are located on the top corners of the front dress panel. -
Page 47: Changing The B2 Prefilter
Operating Instructions Safety Cabinet | 1300 Series B2 Maintaining the Cabinet Changing the B2 Prefilter: Unplug the cabinet or turn off the System Reset Switch located on the top of the cabinet. Using an appropriate ladder or step, loosen and remove the two wing nuts that secure the B2 prefilter in place. -
Page 48: Resetting A Circuit Breaker
Operating Instructions Safety Cabinet | 1300 Series B2 Maintaining the Cabinet Resetting a Circuit Breaker: To reset any of the circuit breakers located on the left side of the electronics module, depress the white button until it sets. Figure 6-4 Circuit Breakers - The front breaker protects the electrical outlets, the rear... -
Page 49: Chapter 7: Troubleshooting
Operating Instructions Safety Cabinet | 1300 Series B2 Troubleshooting Refer to the following table if the Biosafety Cabinet fails to operate properly. If the suggested corrective actions do not solve the problem, contact Thermo Fisher Scientific for additional assistance. PROBLEM CAUSE CORRECTIVE ACTION Cabinet blower and... - Page 50 Operating Instructions Safety Cabinet | 1300 Series B2 Troubleshooting PROBLEM CAUSE CORRECTIVE ACTION UV light not working Sash is open Close sash – UV light will not work with the sash open. Lamp is defective Replace defective lamp. Lamp wiring is Inspect lamp wiring.
- Page 51 Operating Instructions Safety Cabinet | 1300 Series B2 Troubleshooting PROBLEM CAUSE CORRECTIVE ACTION Contamination of work Improper technique or See “Use of the Cabinet” section in the in the cabinet procedure for the manual. Biosafety Cabinet Restriction of the return Ensure that all return air slots, grilles and air slots or grille the exhaust outlet are unobstructed.
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Page 52: Appendix A: Components
Operating Instructions Safety Cabinet | 1300 Series B2 Components Illustration A-1 indicates the location of the following service parts, and replacement accessory parts: Biosafety Cabinet Replacement Parts Item Quantity Part No. Description 3850500 Prefilter for 4-foot models 3850501 Prefilter for 6-foot models 3838501 Exhaust HEPA Filter 4-ft 3838503... -
Page 53: Figure A-1 Service Parts/Replacement Accessory Parts
Operating Instructions Safety Cabinet | 1300 Series B2 Components... -
Page 54: Appendix B: Dimensions
Operating Instructions Safety Cabinet | 1300 Series B2 Dimensions All dimensions in inches. Width “A” “B” “C” 54.3 48.5 17.2 78.3 72.5 28.9... -
Page 55: Appendix C: Specifications
Operating Instructions Safety Cabinet | 1300 Series B2 Specifications Motor Specifications Cabinet Model Electrical Requirements All Cabinets, all Electronically Commutated Motor (ECM) Voltages 120-277 VAC – 50/60 Hz, 7.7 Full Load Amps @115VAC 4.3 Full Load Amps @230VAC 1/2 H.P. 1500 RPM maximum Automatic Thermal Protection Environmental Conditions •... -
Page 56: Appendix D: Accessories
27.5 to 33.5 inches in 1-inch intervals, providing a cabinet work surface height of 30.0 to 36.0 inches. Catalog # For use with Base Stand w/Feet # 1310, 1311, 1315, 4-foot Thermo 1300 Series B2 BSC 3730402 1316, 1317, 1318 1360, 1361, 1362,... -
Page 57: Ulpa Supply Filters
Operating Instructions Safety Cabinet | 1300 Series B2 Accessories *ULPA Supply Filters Rated at 99.999% efficiency with particles 0.12 microns. Catalog # For use with 3859501 All 4-foot Thermo 1300 Series B2 BSC 3859503 All 6-foot Thermo 1300 Series B2 BSC *ULPA Exhaust Filters Rated at 99.999% efficiency with particles 0.12 microns. -
Page 58: Appendix E: Quick Chart
Operating Instructions Safety Cabinet | 1300 Series B2 Quick Chart 1310, 1311, 1360, 1361, Model (Series) 1315, 1316, 1362, 1363, 1317, 1318 1364, 1364 Cabinet Size (in feet) Sash Opening (inches) Starting Serial # 0705_ 0705_ Nominal Avg. Downflow (FPM) -
Page 59: Declaration Of Conformity
See Shipping/Customs Documents for your equipment Type of Equipment: Laboratory Equipment Model No.: 1310, 1311, 1315, 1316, 1317, 1318 4' Wide, 8" Sash, B2 Biological Safety Cabinet 1360, 1361, 1362, 1363, 1364, 1365 6' Wide, 8" Sash, B2 Biological Safety Cabinet Serial No.:...
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