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HERMLE Z 446 K
User Manual
© Hermle Labortechnik GmbH
Z446K_V2.19_eng

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Summary of Contents for Hermle Z 446 K

  • Page 1 HERMLE Z 446 K User Manual © Hermle Labortechnik GmbH Z446K_V2.19_eng...
  • Page 3: Table Of Contents

    Pre-Selection of Speed / RCF-Value ....................... 21 2.3.2 Pre-Selection of Running Time ........................ 22 2.3.3 Pre-Selection of Brake Intensity and Acceleration .................. 23 2.3.4 Pre-Selection of Temperature ......................... 24 2.3.5 Pre-Cooling ............................. 24 Radius Correction .............................25 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 4 Table 2: Permissible Net Weight ........................VI Table 3: Lowest Temperatures at Max. Speed ....................VII Table 4: Max. Speed and RCF-Values for Permissible Rotors ................ VII Table 5: Acceleration and Deceleration Times ....................VIII Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 5 Table 7 (Part 2): Radius Correction........................XI Table 7 (Part 3): Radius Correction........................XII Table 7 (Part 4): Radius Correction......................... XIII Table 7 (Part 5): Radius Correction........................ XIV Table 8: Abbreviations ............................ XV Redemption Form / Decontamination Certificate ..................... XII © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 6: Product Description

    For any resulting damage, the company, Hermle Labortechnik, is not liable. 1.3 Brief Description The unit type Z 446 K, is a refrigerated universal centrifuge, which we offer in two voltage variations 230V or 120V. We offer the 120 V voltage centrifuge as a 15 A and 20 A version.
  • Page 7: Operating And Display Elements

    Control Panel Display rpm/rcf Speed/ g-force accel/decel Acceleration / Deceleration Intensity time Centrifugation Time Lid Release quick Short Running start Start Centrifugation stop Stop Centrifugation prog Retrieving Stored Programs store Program Store temp Temperature Indication © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 8: Lcd-Display

    "error" "rpm" "service" "rcf" h m s "accel" "temperature" Indication: After switching on the centrifuge, the display, "rpm/rcf"(A-1) shows the loading status, the current software version and finally the model type (e.g. Z 36 HK). Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 9: Signs And Indications Of The Centrifuge

    Instructions for Disposal (see P. 34) Direction of Rotation – clockwise rotation for the rotor drive Reference for Loading Rotors 1.6.2 Product Nameplate (Example) Company Address: Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen In-Vitro-Diagnostic Medical Device TYPE: Type Designation of the Product REF: Order No.
  • Page 10: Warning And Information Signs

    EN 61010-2-020. • The HERMLE Z 446 K is "non-explosion proof" and must not be operated in explosion- endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such substances, which chemically react with high energy, is strictly prohibited. If used in such environment, this is at the users own expense.
  • Page 11: Following Rules Must Strictly Be Adhered To

    Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered with, by unauthorized personnel. • Do not use accessories such as rotors and buckets, that are not approved by HERMLE Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or plastic.
  • Page 12: Installation Of The Centrifuge

    1.7 Installation of the Centrifuge 1.7.1 Unpacking the Centrifuge Model Z 446 K is supplied in a carton. Remove the strap retainer, open the carton, and remove the padding. Lift the centrifuge on both sides (seeFigure 1) with an appropriate number of helpers and place it on the laboratory table.
  • Page 13: Attention Before First Use

    If the motor shaft is conical remove the collet from the rotor by turning it The collet is no longer counter clockwise needed If the motor shaft is cylindrical Let the collet inside the rotor © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 14: Basic Adjustments

    All changed settings must be confirmed by the key, "start" (9). A confirmation screen will appear with the word; "store" in the display box, "rpm/rcf"(A-1) - Only then, the pre-selections are valid, (see Figure 3)! Figure 3 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 15: Temperature Indication

    By pressing the key, "rpm/rcf" (4), the word "On" flashes, and the sound can be switched off with the adjustable knob (1), (see Figure 5). After the settings have been stored by user, the normal program mode can be changed back again by switching off the centrifuge, for a short period. Firgure 5 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 16: Volume Pre-Selection Of Sound Signal

    After pressing the key „rpm/rcf“ (4), select a song with the adjusting knob (1), (see Figure 7). After the settings have been stored by user (see 1.8.1), the normal program mode can be changed back again by switching off the centrifuge, for a short period. Figure 7 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 17: Keyboard Sound Turn On / Off

    (1), (see Figure 8). After the settings have been stored by user (see 1.8.1), the normal program mode can be changed back again, by switching off the centrifuge, for a short period. Figure 8 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 18: Retrieving Operating Data (Operated By Skilled Or Service Engineer Only!)

    (1). The respective error codes appear in the display, "rpm/rcf" (A- 1). Please refer to Table 6: "Error Messages“, (see APPENDIX P.VIII). Switch off the centrifuge, to return to the normal program mode. Figure 9 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 19: Operation

    Hold the rotor with one hand and secure the rotor to the shaft, by turning the fixing nut clockwise. Tighten the fixing nut with the provided Allen key, (see Figure 11). Figure 12 ATTENTION: For safety, always ensure that the rotor fixing screw is tightened before each run, (see Figure 11)!! © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 20: Loading Angle Rotors

    The bolts of the rotor must be greased with the HERMLE Rotorgrease (Order No. 38-5656). The sample tubes have to be filled evenly, by eye, and set into the drillings or tube racks. The weight difference of the loaded buckets should not exceed 1.0 g.
  • Page 21: Loading And Overloading Of Rotors

    In case of any questions, please contact the manufacturer! 2.1.5 Removing the Rotor Completely, untighten the rotor fixing nut (2. screw over the stiff point) and lift the rotor vertically out of the centrifuge, (see Figures 10 and 11). © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 22: Lid

    For all number marked text, please refer to Figure 17. ATTENTION: Don't grip your fingers between the lid and the device or the locking mechanism, when closing the lid! Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 23: Pre-Selection

    See Table 4: "Max. Speed and RCF-Values for Permissible Rotor", (see APPENDIX P. VII). All important values are listed on this table. ATTENTION: Please check the maximum permissible revolutions of your test tubes! (Producer Indication). © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 24: Pre-Selection Of Running Time

    The time counts down, as soon as the set speed is reached. The display will always show the remaining running time, (see Figure 19). For all number marked text, please refer to Figure 19. Figure 19 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 25: Pre-Selection Of Brake Intensity And Acceleration

    For all number marked text, please refer to Figure 20. See Table 5: "Acceleration and Deceleration Times", (APPENDIX P. VII). This table shows the acceleration and deceleration times, for the acceleration and deceleration stages 0 to 9, for permissible rotors. Figure 20 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 26: Pre-Selection Of Temperature

    “stop” key (10). Depending on the inserted rotor, the pre-cooling lasts between 10 to 20 min. For all number marked text, please refer to Figure 22. Figure 22 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 27: Radius Correction

    As soon as the radius correction is set, the word "radius" (M9) appears. This text remains visible until the radius correction is set back to 0. For all number marked text, please refer to Figure 23. Figure 23 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 28: Program

    (M10) disappears. As soon as the key "store" (12) is no longer displayed, the word "programm xx" (M10) reappears. ( the xx stands for the chosen program place). If all program numbers are occupied, take an old number that is not needed any longer and replace it with the new parameters. Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 29: Recall Of Stored Programs

    To leave the program mode, press the key, "prog" (11). Then, inside the display "time", appears the word "programm". Set the display to "programm--" (M10) with the adjustable knob (1). For all number marked text, please refer to Figure 27. © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 30: Starting And Stopping The Centrifuge

    With the "stop" key (10), the run time can be interrupted, at any time, (see Figure 29). After pressing the key, the centrifuge decelerates with the respective pre-selected intensity, down to a standstill. Figure 29 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 31: Imbalance Detection

    You can interrupt this function by pressing the bottom “stop” (10) or the bottom “lid” (11). During the timer mode, all the other bottoms have no function. For all number marked text, please refer to Figure 31 and 32. Figure 31 Figure 32 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 32: Maintenance

    "TÜV Nord CERT GmbH, Certification Body Consumer Products, Essen (Germany)", in accordance with Annex AA of IEC 61010-2-020. The certificates can be downloaded on our webpage, www.hermle-labortechnik.de. Aerosol-tight rotors and buckets are marked with the label, "aerosol- tight". ATTENTION: Autoclaving, mechanical stresses and contamination, by chemicals or other aggressive solvents, can impair the aerosol-tightness of the rotors and buckets.
  • Page 33: Cleaning And Disinfecting Of The Unit

    3.1.4 Disinfection of Aluminum Rotors In case of infectious material spilling into the centrifuge, the rotor and rotor chamber have to be disinfected, promptly after the run. Rotors may be autoclaved, at a maximum temperature of 121°C/250°F (2.15 bar). © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 34: Disinfection Of Pp-Rotors

    PP, have a maximum operating time of up to 3 years, from first time use. Plastic lids and seals of aerosol-tight aluminum rotors and aluminum buckets must be replaced after ten autoclaving cycles. Conditions For the Operating Time: Proper use, damage-free condition, recommended care. Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 35: Trouble Shooting

    • Turn the box spanner to the right (clockwise), up to the limit. ATTENTION: Only turn to the limit, don´t tighten the nut. • Open the lid of the centrifuge. • Switch the centrifuge on again, to proceed with regular function. Figure 33 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 36: Description Of The Error Message System

    Once the rotor has come to a standstill, open the centrifuge lid with the emergency release. Proceed as described in chapter 4.2.1, p 26. After opening the centrifuge lid, switch on the device again. Error 14 and the lettering "USEr GuidE" should be eliminated. Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 37: Procedure While Error 38 - Lid Motor Is Blocked

    The lid lock adjusts itself in the basic position. The closing process can be carried out normally again. If these errors occurs again, please contact the service. © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 38: Receipt Of Centrifuges For Repair

    In case of a return of a centrifuge, we also ask you to send its power cord. This eliminates the risk of a faulty power cord. If no power cord is attached to the centrifuge, a new one will be delivered and charged. We ask for your understanding. Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 39: Transport, Storage And Disposal

    For all devices delivered before 13.08.2005, the last user is responsible for proper disposal. RoHS Declaration of Conformity HERMLE Labortechnik GmbH, Siemensstraße 25, 78564 Wehingen, Germany, hereby declares that all components produced are in conformity with Directive 2015/863/EU of the European Parliament and of the Council of 31.3.2015 on the restriction of the use of certain hazardous substances in...
  • Page 41: Appendix

    Table 7 (Part 3): Radius Correction ......................XII Table 7 (Part 4): Radius Correction ......................XIII Table 7 (Part 5): Radius Correction ......................XIV Table 8: Abbreviations ..........................XV Redemption Form / Decontamination Certificate ................... XII © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 42: Eg - Conformity Declaration

    Z 326; Z 327; Z 366; Z 446; Z 207 MK; Z 216 MK; Z 32 HK; Z 326 K; Z 327 K; Z 366 K; Z 36 HK; Z 446 K; ZK 496 Einschlägige EG-Richtlinien / Normen / Relevant EC Directives / Standards (EU) 2017/746;...
  • Page 43 APPENDIX Table 1: Technical Data HERMLE Labortechnik GmbH, 78564 Wehingen Manufacturer Z 446 K Type Dimensions 73 cm Width 67 cm Depth Height 39 cm 111 kg Weight without rotor Max. Speed 16000 min 4 x 750 ml Max. Volume 26331 x g Max.
  • Page 44 221.52 V20 760 g 10500 rpm 221.38 V20 14 g 15000 rpm 221.68 V20 165 g 16000 rpm 220.87 V21 85 g 16000 rpm 220.87 V20 85 g 16000 rpm 220.88 V20 150 g 16000 rpm Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 45 221.52 V02 10000 min-1 16022 xg 221.38 V01 15000 min-1 15343 xg 221.68 V02 16000 min-1 24325 xg 220.87 V16 16000 min-1 24325 xg 220.87 V15 16000 min-1 24325 xg 220.88 V09 16000 min-1 24039 xg © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 46 221.36 V20 1287 221.51 V20 2317 221.52 V20 1747 221.38 V20 221.68 V20 220.87 V21 220.87 V20 220.88 V20 In seconds Acceleration Time Deceleration Time from 0 min -> U from U -> 0 min VIII Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 47 APPENDIX Table 6: Error Messages © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 48 16.7 710.006 16.9 710.007 17.1 710.008 17.1 710.009 16.7 710.010 16.4 710.011 17.1 710.012 17.1 710.013 16.4 710.014 16.4 710.015 17.0 710.016 17.0 710.017 16.9 710.018 16.9 710.019 16.3 710.020 16.9 710.021 16.9 710.022 16.7 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 49 17.3 711.007 17.0 711.008 17.0 711.009 17.2 711.010 16.9 711.011 16.9 713.001 17.1 713.002 17.1 713.003 17.1 713.004 16.8 713.005 16.6 713.006 16.8 713.007 17.1 713.008 17.1 713.009 17.3 713.010 17.1 713.011 17.1 713.022 16.8 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 50 625.010 16.9 625.013 11.9 625.014 17.3 625.015 17.4 625.018 18.1 625.019 16.5 625.020 15.3 625.021 13.5 625.023 18.1 Swing Out Rotor 10.2 221.16 706.000 10.2 Angle Rotor 221.17 704.004 704.005 Angle Rotor 221.68 704.004 704.005 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 51 10.4 Angle Rotor 221.20 707.001 707.002 707.003 707.004 707.014 707.000 14.1 Angle Rotor 221.21 713.015 13.5 713.020 13.3 713.025 713.028 12.5 713.030 713.042 11.7 Angle Rotor 221.22 708.003 708.004 708.017 708.019 Angle Rotor 221.28 XIII © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 52 712.100 13.4 712.101 12.4 712.102 12.6 712.103 13.8 712.104 14.3 712.105 13.8 Angle Rotor 221.38 Angle Rotor 220.87 704.004 704.005 Angle Rotor 220.88 704.005 Angle Rotor 221.52 708.050 12,7 708.051 12,8 701.011 11,4 701.012 701.015 Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 53 APPENDIX Table 8: Abbreviations Symbol/Abbreviations Unit Description U (=rpm) [min Revolutions per Minute RZB(=rcf) [x g] Relative Centrifugal Force Polypropylene Polycarbonate accel Acceleration decel Deceleration prog Program © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 54 Have these substances reached the equipment/assembly? …………………………..  Yes  No If so, which one: ________________________________________________________________ Description of the measures for the decontamination of the listed parts: I confirm the proper decontamination: Company/Dept ._____________ Place and Date: ____________________________________ Signature of the authorized person:________________________________________ Z446K_V1.22_eng © Hermle Labortechnik GmbH...
  • Page 55 © Hermle Labortechnik GmbH Z446K_V1.22_eng...
  • Page 56 HERMLE Labortechnik GmbH Siemensstraße 25 78564 Wehingen Tel: +49 (0) 74 26-96 22-17 Fax: +49 (0) 74 26-96 22-49 Email: vertrieb@hermle-labortechnik.de Internet: http://www.hermle-labortechnik.de Technical rights reserved ©HERMLE Labortechnik GmbH 2019 Z446K_V1.22_eng © Hermle Labortechnik GmbH...

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