Hermle Z 446 User Manual
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Hermle Z 446 User Manual

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HERMLE Z 446
User Manual

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Dr zekari
February 20, 2025

Bonjour, les tubes commencent a se briser à l intérieur de la centri d une manière aléatoire, l équilibre est respecté, ceci est arrivé après le passage des techniciens pour l étalonnage, sachant qu ils ont vérifié la vitesse, le temps et le froid sans toucher au rotor. Merci

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Mr. Anderson
February 20, 2025

Tube breakage inside the Hermle Z 446 centrifuge after calibration, despite maintaining balance, could be caused by several factors:

1. Rotor or Accessory Damage: Even slight damage to rotors, buckets, or tube racks can lead to breakage. Anodized aluminum parts are particularly sensitive.

2. Corrosive Substance Exposure: If rotors, buckets, or tube racks come into contact with corrosive substances like alkalis, alkaline soap solutions, alkaline amines, or concentrated acids, they may weaken and cause breakage.

3. Improper Cleaning and Maintenance: Failing to properly clean and lubricate rotor components, especially aerosol-tight rotor seals and insert bolts, may lead to mechanical stress and tube breakage.

4. Use of Non-Compatible Rotors or Accessories: Only Hermle original rotors and accessories should be used. Non-compatible parts may not function correctly and could cause breakage.

5. Exceeding Sample Load Limits: Overloading or using tubes that are not rated for the centrifuge’s speed or force can result in breakage.

6. Hidden Cracks or Weakness in Tubes: Tubes with pre-existing cracks or defects may break under centrifugal force, even if balance is maintained.

Ensuring proper maintenance, using appropriate accessories, and avoiding corrosive substances can help prevent tube breakage.

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Summary of Contents for Hermle Z 446

  • Page 1 HERMLE Z 446 User Manual...

  • Page 3: Table Of Contents

    Lid Lock ................................. 16 Pre-Selection ..............................17 2.3.1 Pre-Selection of Speed / RCF-Value ......................17 2.3.2 Pre-Selection of Running Time ........................17 2.3.3 Pre-Selection of Brake Intensity and Acceleration ..................18 Radius Correction ............................19 Program ................................19 © Hermle Labortechnik GmbH Z446_V2.21_eng...
  • Page 4 EG - Conformity Declaration ............................ V Table 1: Technical Data ............................VII Table 2: Permissible Net Weight ........................... VIII Table 3: Max. Speed and RCF-Values for Permissible Rotors ................VIII Table 4: Acceleration and Deceleration Times ......................IX Z446_V2.21_eng © Hermle Labortechnik GmbH...
  • Page 5 Table 6 (Part 3): Radius Correction........................XIII Table 6 (part 4): Radius Correction ........................XIV Table 6 (part 5): Radius Correction ........................XV Table 7: Abbreviations ............................XV Redemption Form / Decontamination Certificate ....................XVII © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 7: Product Description

    For any resulting damage, the company, Hermle Labortechnik, is not liable. 1.3 Brief Description The unit type Z 446, is a non refrigerated universal centrifuge, which we offer in two voltage variations 230V or 120V. The centrifuge can be used with swing-out rotors and angle rotors.

  • Page 8: Operating And Display Elements

    Run Parameters Power Switch Control Panel Display rpm/rcf Speed/ g-force accel/decel Acceleration / Deceleration Intensity time Centrifugation Time Lid Release quick Short Running start Start Centrifugation stop Stop Centrifugation prog Retrieving Programs store Program Store Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 9: Lcd-Display

    Rotor-No. "error" "rpm" "service" "rcf" h m s "accel" Indication: After switching on the centrifuge, the display, "rpm/rcf"(A-1) shows the loading status, the current software version and finally the model type (e.g. Z 36 HK). © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 10: Signs And Indications Of The Centrifuge

    Instructions for Disposal (see 31) Direction of Rotation – clockwise rotation for the rotor drive Reference for Loading Rotors 1.6.2 Product Nameplate (Example) Company Address: Hermle Labortechnik GmbH, Siemensstr. 25, D-78564 Wehingen TYPE: Type Designation of the Product REF: Order No. of the Product Serial No.

  • Page 11: Warning And Information Signs

    EN 61010-2-020. • The HERMLE Z 446, is "non-explosion proof" and must not be operated in explosion- endangered areas or locations. Centrifugation of flammable, explosive, radioactive, or such substances, which chemically react with high energy, is strictly prohibited. If used in such environment, this is at the users own expense.

  • Page 12: Following Rules Must Strictly Be Adhered To

    Do not run the centrifuge, if mechanical or electrical assembly groups have been tampered with, by unauthorized personnel. • Do not use accessories such as rotors and buckets, that are not approved by HERMLE Labortechnik GmbH, except commercially available centrifuge tubes, made of glass or plastic.

  • Page 13: Installation Of The Centrifuge

    1.7 Installation of the Centrifuge 1.7.1 Unpacking the Centrifuge Model Z 446 is supplied in a carton. Remove the strap retainer, open the carton, and remove the padding. Lift the centrifuge on both sides (see Figure 1) with an appropriate number of helpers and place it on the laboratory table.

  • Page 14: Attention Before First Use

    If the motor shaft is conical remove the collet from the rotor by turning it The collet is no longer counter clockwise needed If the motor shaft is cylindrical Let the collet inside the rotor Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 15: Basic Adjustments

    All changed settings must be confirmed by the key, "start" (9). A confirmation screen will appear with the word; "store" in the display box, "rpm/rcf"(A-1) - Only then, the pre-selections are valid, (see Figure 3 Figure 3 © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 16: Sound Signal Turn On / Off

    9 (loud), with the adjustable knob (1), (see FFigure 5). After the settings have been stored by user (see 1.8.1), the normal program mode can be changed back again by switching off the centrifuge, for a short period. Figure 5 Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 17: Song Selection - End Of Run

    (1), (see Figure 7). After the settings have been stored by user (see 1.8.1), the normal program mode can be changed back again, by switching off the centrifuge, for a short period. Figure 7 © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 18: Retrieving Operating Data (Operated By Skilled Or Service Engineer Only!)

    (1). The respective error codes appear in the display, "rpm/rcf" (A- 1). Please refer to Table 5: "Error Messages“, (see APPENDIX P.VIII). Switch off the centrifuge, to return to the normal program mode. Figure 8 Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 19: Operation

    Each rotor indicates what the maximum capacity is per hole. (It is allowed to operate e.g. a 12-place- rotor with 2 or 4 loaded tubes only, but the loaded borings must be opposite of each other). © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 20: Loading Swing Out Rotors

    In principle, swing out rotors cannot be removed during operation, until all buckets or racks are placed inside the rotor. The bolts of the rotor must be greased with the HERMLE Lubricatinggrease (Order No. 38-5656). The sample tubes have to be filled evenly, by eye, and set into the drillings or tube racks.

  • Page 21: Loading And Overloading Of Rotors

    In case of any questions, please contact the manufacturer! 2.1.5 Removing the Rotor Completely, untighten the rotor fixing nut (2. screw over the stiff point) and lift the rotor vertically out of the centrifuge, (see Figures 9 and 10). © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 22: Lid

    For all number marked text, please refer to Figure 16. ATTENTION: Don't grip your fingers between the lid and the device or the locking mechanism, when closing the lid! Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 23: Pre-Selection

    3. Range: Continuous Run "cont", can be interrupted by the key, "stop" (10). - The running time can be pre-selected, with the lid opened or closed. - To activate the setting of the running time, press the key "time" (6). © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 24: Pre-Selection Of Brake Intensity And Acceleration

    For all number marked text, please refer to Figure 19. See Table 4: "Acceleration and Deceleration Times", (APPENDIX P. VII). This table shows the acceleration and deceleration times, for the acceleration and deceleration stages 0 to 9, for permissible rotors. Figure 19 Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 25: Radius Correction

    If a program number is already occupied, in the display "rpm/rcf" (A-1), the words "rotor" (M3) and "22x.xx" (M4) will appear, (see Figure 21). Free program numbers will appear as 0. Figure 21 © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 26: Recall Of Stored Programs

    "rpm/rcf" (A-1) flashes the word "rotor" (M3). At the same time, the word "FALSE" and the stored rotor number "22x. xx“"(M4) flash in sequence of one another. For all number marked text, please refer to Figure 24. Figure 24 Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 27: Leaving Program Mode

    With the "stop" key (10), the run time can be interrupted, at any time, (see Figure 26). After pressing the key, the centrifuge decelerates with the respective pre-selected intensity, down to a standstill. Figure 26 © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 28: Imbalance Detection

    „rpm/rcf“ (4). The display „time“ (A-3) shows the remaining time until the start of the centrifugation. The timer starts automatically after setting the needed value. The set time can be adjusted by pressing again the bottom “time” (6) and turning the adjusting knob (1). Figure 28 Figure 29 Z446_V2.21_eng © Hermle Labortechnik GmbH...
  • Page 29 You can interrupt this function by pressing the bottom “stop” (10) or the bottom “lid” (11). During the timer mode, all the other bottoms have no function. For all number marked text, please refer to Figure 28 and 29. © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 30: Maintenance

    Following, lubricate the bolts or sealing, with the recommended HERMLE Rotorgrease - Order No.: 38-5656. Please pay special attention to anodized aluminum parts. Breakage of rotors can be caused by the slightest damages.

  • Page 31: Cleaning And Disinfecting Of The Unit

    At high temperatures, autoclaving residue may corrode and destroy the plastic. The objects must be thoroughly washed with distilled water, after the cleaning, but before the autoclaving. Residues of any cleaning liquids, may cause fissures, whitening and stains. © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 32: Glass Breakage

    "TÜV Nord CERT GmbH, Certification Body Consumer Products, Essen (Germany)", in accordance with Annex AA of IEC 61010-2-020. The certificates can be downloaded on our webpage, www.hermle-labortechnik.de. Aerosol-tight rotors and buckets are marked with the label, "aerosol- tight". ATTENTION: Autoclaving, mechanical stresses and contamination, by chemicals or other aggressive solvents, can impair the aerosol-tightness of the rotors and buckets.

  • Page 33: Trouble Shooting

    • Turn the box spanner to the right (clockwise), up to the limit. ATTENTION: Only turn to the limit, don´t tighten the nut. • Open the lid of the centrifuge. • Switch the centrifuge on again, to proceed with regular function. © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 34: Description Of The Error Message System

    If Error 14 occurs, there is a problem with the speed sensor. The centrifuge lid is closed for undefined period of time and in the "rpm/rcf" (A-1) display shows the lettering "USEr GuidE" (see Figure 32). Figure 32 Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 35: Procedure While Error 38 - Lid Motor Is Blocked

    Proceed as described in chapter 4.2.1. After opening the centrifuge lid, switch on again the device. The lid lock adjusts itself in the basic position. The closing process can be carried out normally again. © Hermle Labortechnik GmbH Z446_V2.21_eng...

  • Page 36: Receipt Of Centrifuges For Repair

    In case of a return of a centrifuge, we also ask you to send its power cord. This eliminates the risk of a faulty power cord. If no power cord is attached to the centrifuge, a new one will be delivered and charged. We ask for your understanding. Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 37: Transport, Storage And Disposal

    RoHS II Compliance HERMLE Labortechnik GmbH, Siemensstraße 25, 78564 Wehingen, hereby declares and certifies that all components manufactured are RoHS II compliant, according to the definition and restrictions given by the European Parliament Directive 2011/65/EC. This restricts the use of certain hazardous substances in electrical and electronic equipment.
  • Page 38 TRANSPORT, STORAGE AND DISPOSAL Z446_V2.21_eng © Hermle Labortechnik GmbH...

  • Page 40: Appendix

    APPENDIX 7. APPENDIX EG - Conformity Declaration ....................V Table 1: Technical Data..................... VII Table 2: Permissible Net Weight ..................VIII Table 3: Max. Speed and RCF-Values for Permissible Rotors ........VIII Table 4: Acceleration and Deceleration Times ..............IX Table 5: Error Messages .....................

  • Page 41: Eg - Conformity Declaration

    Z 206 A; Z 207 A; Z 207 H, Z 167 M; Z 207 M, Z 216 M; Z 287 A, Z 306; Z 326; Z 366; Z 446; Z 207 MK; Z 216 MK; Z 32 HK; Z 326 K; Z 366 K; Z 36 HK; Z 446 K, ZK 496 Einschlägige EG-Richtlinien / Normen...
  • Page 42 APPENDIX Z446_V2.21_eng...
  • Page 43 APPENDIX Table 1: Technical Data HERMLE Labortechnik GmbH, 78564 Wehingen Manufacturer Z 446 Type Dimensions 54 cm Width 67 cm Depth Height 39 cm 79 kg Weight without rotor Max. Speed 16000 min 4 x 750 ml Max. Volume 24325 x g Max.
  • Page 44 APPENDIX Table 2: Permissible Net Weight Rotor- Max. Speed Number Value 4500 min-1 1420 g 220.86 V20 4500 min-1 2832 g 221.08 V20 4500 min-1 620 g 221.16 V20 15000 min-1 106 g 221.17 V20 11000 min-1 840 g 221.18 V20 15000 min-1 560 g 221.20 V20...
  • Page 45 APPENDIX Table 4: Acceleration and Deceleration Times Acceleration Values Deceleration Values Level 0 Level 9 Level 0 Level 9 220.86 V20 221.08 V20 221.16 V20 221.17 V20 1654 221.18 V20 1251 221.20 V20 1903 221.21 V20 221.22 V20 221.28 V20 1287 221.36 V20 2317...
  • Page 46 APPENDIX Table 5: Error Messages Z446_V2.21_eng...
  • Page 47 APPENDIX Table 6 (Part 1): Radius Correction Rotor No. Adapter/Tuberack Radius Correction Order-No. (cm) (cm) 610.000 17.4 Swing out Rotor 220.86 Rectangle Bucket 710.000 17.1 710.001 17.4 710.002 16.8 710.003 16.9 710.004 16.7 710.005 16.7 710.006 16.9 710.007 17.1 710.008 17.1 710.009 16.7...
  • Page 48 APPENDIX Table 6 (Part 2): Radius Correction Rotor No. Adapter/Tuberack Radius (cm) Correction Order-No. (cm) Swing Out Rotor 611.000 17,3 220.86 Round Bucket 711.003 17.0 711.004 17.0 711.005 17.1 711.006 17.3 711.007 17.0 711.008 17.0 711.009 17.2 711.010 16.9 711.011 16.9 713.001 17.1...
  • Page 49 APPENDIX Table 6 (Part 3): Radius Correction Rotor No. Adapter/Tuberack Radius (cm) Correction Order-No. (cm) 625.006 18.2 Swing Out Rotor 221.08 625.000 18.2 625.001 18.0 625.002 18.1 625.003 17.9 625.004 17.7 625.005 17.0 625.006 18.3 625.007 18.3 625.008 18.4 707.002 707.003 707.004 707.014...
  • Page 50 APPENDIX Table 6 (part 4): Radius Correction Rotor No. Adapter/Tuberack Radius (cm) Correction Order-No. (cm) 616.100 19.3 Swing Out Rotor 221.36 716.016 18.7 716.018 18.8 716.020 19.1 716.021 19.1 716.023 19.1 Round Bucket 716.024 18.9 716.100 19.1 716.101 19.1 716.102 19.1 716.103 19.2...
  • Page 51 APPENDIX Table 6 (part 5): Radius Correction Rotor No. Adapter/Tuberack Radius (cm) Correction Order-No. (cm) Angle Rotor 221.52 708.050 12,7 708.051 12,8 701.011 11,4 701.012 701.015 Table 7: Abbreviations Symbol / Abbreviation Unit Description U (U =rpm) [min ] Revolutions per Minute RZB(=rcf) [x g] Relative Centrifugal Force...
  • Page 52 APPENDIX Z446_V2.21_eng © Hermle Labortechnik GmbH...
  • Page 53 Have these substances reached the equipment/assembly? …………………………..  Yes  No If so, which ones: ________________________________________________________________ Description of the measures for the decontamination of the listed parts: I confirm the proper decontamination: Company/Dept ._____________ Place and Date: ____________________________________ Signature of the authorized person: _________________________________ XVII © Hermle Labortechnik GmbH Z446_V2.21_eng...
  • Page 54 APPENDIX XVIII Z446_V2.21_eng © Hermle Labortechnik GmbH...
  • Page 55 © Hermle Labortechnik GmbH Z446_V2.19...
  • Page 56 HERMLE Labortechnik GmbH Siemensstraße 25 78564 Wehingen Tel: +49 (0) 74 26-96 22-17 Fax: +49 (0) 74 26-96 22-49 Email: vertrieb@hermleLT.de Internet: http://www.hermle-labortechnik.de Technical rights reserved ©HERMLE Labortechnik GmbH 2019 Z446_V2.21_eng © Hermle Labortechnik GmbH...

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