Malfunction; In-Built Recording - Leica GLOW800 User Manual

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13.5 Malfunction

Observation
No GLOW image is displayed
No sharp GLOW800 image on the monitor in low
magnification, but the White Light image is
sharp, even in low magnifications
Disturbing fluorescence signals especially at the
outer field of view
GLOW fluorescence video image is imbued over
the full field of view
The flow signal appears dark on the visualization
device.

13.6 In-built recording

Observation
No GLOW fluorescence image on the monitor
although it worked before
32
Cause
GLOW800 mode is not active
The fluorescence focal plane is misaligned.
Ambient NIR light >800 nm is detected by the
GLOW camera in outer FoV whereas the focus
point is seated lower
The GLOW illumination touches the cavity border
line and produces disturbing fluorescence
artefacts
Ambient NIR light of an OR light source or OR
room illumination reaches the object field and is
detected by the fluorescence camera over the
whole FoV
A viewing position below and/or laterally off the
visualization device axis get the observed image
darker.
Cause
No more disk space on the ARveo 8 recording
unit.
GLOW800 / Ref. 10 748 652 / Version 01
Remedy
Check that the fluorescence LED and the
X
control unit display the GLOW800 mode.
Check whether the GLOW800 function is
X
assigned to the intended button and handle.
Use test card for proper testing.
X
Call the Leica service if the issue persists.
X
Contact Leica Service.
X
Switch the external light source off, which
X
produces NIR radiation in the GLOW800
detected spectral range.
Close the illumination iris to avoid the
X
illumination of non-interesting areas.
Switch external NIR light source off.
X
For detection of this light source in the OR
X
switch the microscope illumination off and
focus the microscope in GLOW mode on a
white paper.
As long the false signal is present the
X
ambient NIR light is active. Switch the OR
light sources off one after the other until the
issue is gone.
Align the visualization device axis to fit your
X
observation direction.
Remedy
Check the capacity of the ARveo 8 recording
X
unit:
If the ARveo 8 recording unit disk space is full,
X
save and delete unused user data to generate
sufficient capacity.

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