Thermo Scientific CryoStar NX50 Operator's Manual
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Thermo Scientific CryoStar NX50 Operator's Manual

Thermo Scientific CryoStar NX50 Operator's Manual

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Thermo Scientific CryoStar NX50
  
 
  
 
Operator Guide
388149 Issue 2.0
  
  
  
  

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Summary of Contents for Thermo Scientific CryoStar NX50

  • Page 1 Thermo Scientific CryoStar NX50 Operator Guide 388149 Issue 2.0                                    ...
  • Page 2 Company Information   2 | Thermo Scientific NX50...

  • Page 3: Company Information

    Thermo Fisher Scientific makes every attempt to ensure that the information contained in this supporting document is correct and clearly stated, but does not accept responsibility for any errors or omissions. The development of Thermo Scientific products and services is an ongoing process. Please ensure that any published information you use as a reference is up to date and relates to the condition of the product.

  • Page 4: Important Note Regarding Instrument Variants

    Company Information Important Note Regarding Instrument Variants This document is designed for use with the Thermo Scientific CryoStar NX50 cryostat also referred to as the CryoStar NX50 throughout this Guide. A range of optional features are available on this instrument and the instructions contained in this manual are not specific to any one variant.

  • Page 5: Symbols

    This is to be applied in the countries of the European Union and other European countries with a separate collecting system within the waste management. This product, being an electro and/or electronic instrument, must be treated separately within the waste management process (WEEE).      Manufacturer.      Authorised European Representative.   NX50 5 | Thermo Scientific...

  • Page 6: Table Of Contents

    Specimen Head NX50 ..........................18  Set Up ................................ 19  Fitting or Exchanging the Anti Roll Plate ....................19  Fitting or Exchanging Low Profile Blades ....................20  Removing Used Blades with the Magnetic Blade Tool ................21  6 | Thermo Scientific NX50...
  • Page 7 Programming the Daily Defrost Cycle ......................41  Chamber Light ............................42  Screen ................................. 43  Display Lock .............................. 43  Retraction ..............................44  Data Log ..............................44  Factory Settings ............................45  Service ................................ 46  User Guide ..............................46  NX50 7 | Thermo Scientific...
  • Page 8 Conditions for the Transportation of the Instrument ................. 65  Appendices ................................. 69  Appendix A - Spares and Accessories ....................... 69  Appendix B - Recommended Disinfection Procedure ..................71  Appendix C - Cold Disinfection Certificate ....................72  Index .................................. 73  8 | Thermo Scientific NX50...

  • Page 9: Safety Information

    Mains supply at the source by removing the plug from the socket.     Use only factory approved accessories or replacement parts within the instrument.     Only use reagents recommended in the operator guide. NX50 9 | Thermo Scientific...

  • Page 10: Chemical Safety

    This instrument is modified in any way, or not used as intended by Thermo Fisher Scientific.  Accessories and reagents which have not been approved by Thermo Fisher Scientific are used.  The instrument is not operated or maintained in accordance with instructions. 10 | Thermo Scientific NX50...

  • Page 11: Introduction

    2 - Introduction Intended Use The Thermo Scientific CryoStar NX50 is a high-performance in vitro diagnostic cryostat designed to prepare tissue sections of frozen samples in medical, pharmaceutical and research laboratories. Only qualified and trained laboratory personnel may operate the CryoStar NX50.

  • Page 12: Technical Specifications

    Up to 2000 m (6,500 ft) Pollution Degree Over Voltage Category Floor Loading Requirements 300 kg/m² (660 lb/ft²) Safety Class <43 dB (A) - measured at 1m from Sound Emission instrument Refrigerant R404a, filling amount 400g 12 | Thermo Scientific NX50...

  • Page 13: Cooling Specifications

    - and y - axes universal 8° with zero positioning; z - axis up to 360° Coarse Feed Motorized Cutting Drive Manual via hand wheel Clearance Angle Adjustment Disposable blade carrier 8- 16° Vacutome Filter approximately 500 ml NX50 13 | Thermo Scientific...

  • Page 14: Cold D Specification

    5 μm Chamber Light 100% Screen Brightness 100% Stepper Motor Initialization Specimen Temperature -20°C Cryobar Vacutome (optinal) Disinfection (optional) Disinfection, Cycle (Automatic) ON (03:00 Night) Defrost Cycle (Automatic) ON (00:00 Night) Section Counter Section Sum   14 | Thermo Scientific NX50...

  • Page 15: Instrument Setup

     The instrument can be moved on its castors. However, ensure that it does not roll off the pallet inadvertently.  Screw in the two bails (part of the standard accessories) into the respective holes in the foot of the CryoStar NX50.

  • Page 16: Repacking The Instrument

    Technical Specifications (Chapter 2) can reduce the performance of the instrument. Once the CryoStar NX50 has been located in its final position, ensure the fixing bolts as shown in diagram below are tightened to prevent the unit from moving during operation.

  • Page 17: Identification Of Parts

    Connections Panel Hand wheel Hand wheel Mechanical Brake Water Drain Reservoir Cover Connections Panel Power Switch Power Socket Rating and Serial Number Label X2 – USB port (to be used with memory stick provided)   NX50 17 | Thermo Scientific...

  • Page 18: Cryochamber

    Blade Holder Base Blade Holder Base Anti-Roll Plate Adjustment Clearance Angle Adjusting Screw Specimen Head NX50 Specimen Chuck Release Lever Orientation Handle Specimen Head Clamping Lever Upper Specimen Chuck Jaw (Static) Lower Specimen Chuck Jaw (Moving)   18 | Thermo Scientific NX50...

  • Page 19: Set Up

     Position the Anti Roll Plate in the holder, gently pushing the glass to the bottom of the holder. Loosen the black screws if needed.  Tighten the black screws. Note There are two usable edges on the Anti Roll Plate.    NX50 19 | Thermo Scientific...

  • Page 20: Fitting Or Exchanging Low Profile Blades

    Pull the blade clamping lever towards the front of the instrument to release the clamping plate.  Carefully slide a low profile blade into the middle of the slot, ensuring that the blade is equidistant from the ends of the slot. 20 | Thermo Scientific NX50...

  • Page 21: Removing Used Blades With The Magnetic Blade Tool

     Move the magnetic blade tool over the blade.  Carefully lift the blade out of the blade holder and dispose of it in accordance with laboratory regulations.      NX50 21 | Thermo Scientific...

  • Page 22: Blade Positioning And Orientation

    The cutting angle can be adjusted. The recommended cutting angle is approximately 10° for Thermo Scientific low profile blades. Note The factory preset cutting angle is 10°, this value is the recommended cutting angle for Thermo Scientific low profile blades. The minimum angle is 8°, the maximum angle is 16°. 22 | Thermo Scientific...
  • Page 23 The clearance angle can be read on the scale on the left side on the upper part of the blade holder. Note 10° is indicated by the longer increment in the middle of the scale.  Re-tighten the clearance angle adjusting screw to fix the selected clearance angle. NX50 23 | Thermo Scientific...

  • Page 24: Adjusting The Anti-Roll Plate

    Push down on the specimen chuck release lever again to allow the jaws to grip the chuck.  Loosen the specimen head clamping lever to allow adjustment of the specimen parallel to the cutting edge. 24 | Thermo Scientific NX50...
  • Page 25 Use the motorized coarse feed to position the blade close to the specimen.  Use the Forward knife movement button to move the blade holder towards the specimen.       To avoid severe damage ensure that the blade does not crash into the specimen. NX50 25 | Thermo Scientific...

  • Page 26: Filling With Disinfectant To Perform The Cold D Function (Optional)

    The Cold D function is an integrated system for the delivery of disinfectant (Sanosil®) into the cold chamber. This method of disinfection provides an even distribution of the disinfectant throughout the CryoStar NX50 chamber. The Cold D cycle takes 50 minutes to complete. This cycle can be aborted at any time although precautions should be taken as the chamber has not been completely disinfected.
  • Page 27 If the fluid level in the disinfectant tank is too low, an error message will be shown on the touch screen display. Dispose of the empty disinfectant bottle in accordance with laboratory regulations. NX50 27 | Thermo Scientific...

  • Page 28: Operation

    Switch off the instrument by using the main switch at the back of the instrument on the right hand side. The window must be opened when you switch the CryoStar NX50 off in order to evaporate the water into ambient air.

  • Page 29: System Interfacing

    System Interfacing Using the Touchscreen The CryoStar NX50 has an embedded touchscreen user interface which is used to set all preferences. Avoid using sharp or pointed objects to press buttons on the touchscreen. It is recommended a clean finger is used to operate the touch screen. However, if you require a stylus, the eraser tipped end of a pencil is siutable.

  • Page 30: Trimming And Fine Section Thickness

     For details of the thickness steps see Technical Specifications  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button to exit back to the Main screen without saving the changes. 30 | Thermo Scientific NX50...

  • Page 31: Temperature

    The cutting window allows the user to customise this setting for each specimen. Note The optional Vacutome function only operates when a cutting window has been set.  On the Main screen press the "Cutting Window" button. NX50 31 | Thermo Scientific...

  • Page 32: Knife Assembly Positioning

    Press the button to move the blade/knife holder in direction to the specimen.  The blade/knife holder will only move while the button is pressed.  It will stop immediately if not pressed. Forward fast (3mm/sec) button 32 | Thermo Scientific NX50...
  • Page 33  Press the button to move the blade/knife holder quickly in direction to the specimen.  The blade/knife holder will only move while the button is pressed.  It will stop immediately when released.   NX50 33 | Thermo Scientific...

  • Page 34: Quick Freezing Of The Specimen

     The Vacutome system is provided with two different-length nozzles. The longer nozzle is optimized for use in specimen trimming and aids in removal of debris from the cyrochamber. The shorter nozzle is optimized for use with the anti-roll plate in appropriately stretching specimens. 34 | Thermo Scientific NX50...
  • Page 35  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button to exit back to the Main screen without saving the changes.    NX50 35 | Thermo Scientific...
  • Page 36 Chapter 4 - Operation Turning On the cleaning mode To activate the cleaning mode, press the "Vacutome" button on the Main screen: Note Use the cleaning hose for cleaning the CryoStar NX50 chamber.  The Vacutome screen will be displayed ...

  • Page 37: Height Adjustment (Optional)

    Chapter 4 – Operation Height Adjustment (optional) The CryoStar NX50 has an optional electronic height adjustment which enables the user to raise or lower the CryoStar NX50 to a convenient working height. Ensure that the area to the rear of the CryoStar NX50 is free of obstructions to avoid damaging the instrument when the height adjustment is in use.

  • Page 38: Time And Date

    It is recommended to reset the section thickness sum if a new specimen is mounted in the specimen head for sectioning. Section Counter  The section counter adds up the number of the produced sections. After each downward movement of the specimen head, the number on the section counter increases by. 38 | Thermo Scientific NX50...

  • Page 39: Menu

     Select the desired language by pressing the respective button.  Press "OK" to save the changes and to return to the Main screen.  Press the "Back" button three times to return to the Main screen without saving the changes.   NX50 39 | Thermo Scientific...

  • Page 40: Standby

    Chapter 4 - Operation Standby When the CryoStar NX50 is switched on but has been inactive for 1 hour, it will automatically enter the Standby mode. The CryoStar NX50 maintains the user adjusted temperatures for specimen head during Standby. In Standby mode the chamber light switches off and the touch screen displays the Thermo Scientific logo.

  • Page 41: Programming The Daily Defrost Cycle

    The time for the daily defrost cycle should be programmed outside of the normal working hours to avoid delays in the workflow. To program the Daily Defrost Cycle:  Press the "Menu" button on the Main screen.  The Menu screen will be displayed.  Press the "Defrost" button. NX50 41 | Thermo Scientific...

  • Page 42: Chamber Light

    100%) or adjust the brightness using the arrow keys.  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button twice to exit back to the Main screen without saving the changes. 42 | Thermo Scientific NX50...

  • Page 43: Screen

    Press “Display Lock” to activate the display lock immediately. To deactivate the Display Lock:  Press the "Lock" Symbol three times. Note The symbol changes its colour (red - yellow - green) each time it is pressed.   NX50 43 | Thermo Scientific...

  • Page 44: Retraction

    USB-Stick for Service purposes in case of a malfunction. To enter the Data Log:  Press the "Menu" button on the Main screen. The Menu screen will be displayed.  Press the "Data Log" button. 44 | Thermo Scientific NX50...

  • Page 45: Factory Settings

    Press the "Back" button three times to return to the Main screen without restoring the factory settings. Note A message appears on the Main screen to inform the user that the factory settings have been restored. The message disappears after approximately 3 seconds.   NX50 45 | Thermo Scientific...

  • Page 46: Service

    Press the "Menu" button on the Main screen. The menu screen will appear.  Press the arrow key. A second menu screen will be displayed.  Press the “User Guide” button. The User Guide screen menu will be displayed. 46 | Thermo Scientific NX50...

  • Page 47: Time And Date

    Press the "Menu" button on the Main screen. The Menu screen will be displayed.  Press the arrow key. A second Menu screen will be displayed. Set the Date  Press the "Date" button. The Date screen will appear. NX50 47 | Thermo Scientific...
  • Page 48 ISO format.  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button three times to exit back to the Main screen without saving the changes.   48 | Thermo Scientific NX50...

  • Page 49: Setting The Time

    The 12/24 button is highlighted in yellow if the 12h mode is active.  Press "OK" to confirm the setting and to return to the Main screen.  Press the "Back" button three times to exit back to the NX50 49 | Thermo Scientific...

  • Page 50: Sectioning Procedures

    The blade holder will move forwards by the preset trim thickness value every cycle. Note The Trim button operates independently of the handwheel. You do not need to turn the handwheel to use the Trim button.   50 | Thermo Scientific NX50...

  • Page 51: Sectioning And Taking Off Sections

    Use the handle to move the anti-roll plate assembly away from the specimen.  The section should remain on the clamping plate.  Arrange specimen section gently with a brush.  Transfer the specimen onto a glass microscope slide with help of adhesion forces.   NX50 51 | Thermo Scientific...

  • Page 52: Hand Brake Lock

    If the slider is not in the correct position to lock, keep the slider pressed and move the handwheel slowly forward and backwards until the slider is locked. To unlock the mechanical Handwheel look:  Pull out the hand brake lock switch. 52 | Thermo Scientific NX50...

  • Page 53: Cold D (Optional)

    Pressing the "Now" button, immediately starts the disinfection of the cryostat chamber, regardless of the programmed times.  The Disinfection Remaining Time screen will appear. Note After the disinfection cycle is completed, the Disinfection Remaining Time screen will disappear and the Main screen will be displayed. NX50 53 | Thermo Scientific...

  • Page 54: Aborting The Disinfection

    In addition to running the on demand Cold D cycle automatic Cold D cycles can be programmed. Follow the directions below to activate (or deactivate) the automatic Cold D feature.  Press the "Menu" button on the Main screen.  Press the "Disinfection" button. 54 | Thermo Scientific NX50...
  • Page 55  When all settings have been done, press "OK" to save the settings and to return to the Main screen.  Press the "Back" button to return to the Setting screen without saving the changes. NX50 55 | Thermo Scientific...
  • Page 56 56 | Thermo Scientific NX50...

  • Page 57: Troubleshooting

    Check & tighten all 1. Knife holder not correctly clamped. stages of knife holder. Specimen and section chatter Blade clamping force can be increased by 2. Blade incorrectly clamped. tightening clamp screw at the rear of NX50 57 | Thermo Scientific...

  • Page 58: Tips For Successful Sectioning

    Adjustment of proper clearance angle. Select a clearance angle of 8-16°. The preset clearance angle is 10°.  Select an appropriate cutting speed: The harder the material, the slower the cutting speed!  Take care when bringing knife and specimen together.   58 | Thermo Scientific NX50...

  • Page 59: How To Avoid Malfunctions When Working With The Vacutome

    -30°C or below Bone Marrow -20°C Brain -12°C Breast -25°C Breast with Fat -30°C or below Cervix -20°C Connective Tissue -16°C -20°C Heart -18°C Kidney -15°C -13°C Liver -13°C Lung -15°C Lymph Node -13°C Muscle -16°C NX50 59 | Thermo Scientific...
  • Page 60 Chapter 5 - Troubleshooting Omentum -35°C Ovary -20°C Pancreas -20°C Prostate -20°C Skin -16°C Skin with fat -25°C Spleen -16°C Testis -10°C Thyroid -15°C Uterine curetting -7°C Uterus -20°C     60 | Thermo Scientific NX50...

  • Page 61: Cleaning And Maintenance

     As there is condensate humidity inside the microtome, dry the microtome components inside the cooling chamber very carefully. For this, use a hair dryer. Note For the examination and readjustment of the microtome a routine maintenance should be performed by trained service technician once a year.     NX50 61 | Thermo Scientific...

  • Page 62: Emptying The Defrosting And Cold D Liquids

    Note After cleaning the bottle, it is recommended that a small volume of Sanosil® is added.  Wipe out any liquids from the rack.  Put the bottle back into the rack. 62 | Thermo Scientific NX50...

  • Page 63: Exchange The Vacutome Filter Bag

    The Vacutome Filter Bag is placed under the cover on the right hand site of the cryochamber.  Open the lever of the cover.  Remove the vacutome hose and lift off the cover.  Clean the vacutome hose with a flexible brush.  Slip off the old vacutome filter. NX50 63 | Thermo Scientific...
  • Page 64 Replace the vacutome filter for a new one.  Clean and disinfect the empty vacutome filter chamber with disinfectant.  Replace the cover, rear end first.  Reattach the vacutome hose.  Lock the cover with the lever. 64 | Thermo Scientific NX50...

  • Page 65: Adjustment Of The Movable Specimen Head Jaw

    Remove the section waste tray, the brush shelf, the blade holder and all other accessories as well as tools from the cryo chamber.  Dispose of the defrosting and Cold D liquids in accordance to the laboratory regulations and clean the bottle. - see Emptying the Defrosting and Cold D Liquids. NX50 65 | Thermo Scientific...
  • Page 66 Two people are necessary for the transport of the instrument. Bringing the instrument back into service After moving the CryoStar NX50, wait at least 8 hours before switching on to allow the refrigerant to settle – Failure to do so may cause damage to the unit.
  • Page 67  Recycle your instrument to support the sustainable recycling of material resources.  Industrial users should contact their suppliers and observe the conditions of the contract. This product must not be disposed of together with other commercial waste.   NX50 67 | Thermo Scientific...
  • Page 68 68 | Thermo Scientific NX50...

  • Page 69: Appendices

    Small brush, magnetic 334171 Large brush, magnetic 334172 Pipe brush 400 x 25 x 14 mm 202180 Coarse filter for use with Vacutome (pk of 25) 281000 Nozzle (for trimming) 336090 Nozzle (for sectioning) 336080 NX50 69 | Thermo Scientific...
  • Page 70 Operator guide Italian 388150 Operator guide French 388151 Operator guide Russian 388156 Operator guide Dutch 388168 Operator guide Chinese 388154 Operator guide Spanish 388152 Operator guide Japanese 388153 Measuring jug 334220 Funnel 334240 USB Stick 612050 70 | Thermo Scientific NX50...

  • Page 71: Appendix B - Recommended Disinfection Procedure

    To guarantee functionality and reliability of the disinfection unit, it is highly recommended to use only Sanosil. When using other disinfection media, Thermo Fisher Scientific Microm International GmbH assumes no liability concerning functionality and disinfection effectiveness. NX50 71 | Thermo Scientific...

  • Page 72: Appendix C - Cold Disinfection Certificate

    Appendices Appendix C - Cold Disinfection Certificate        72 | Thermo Scientific NX50...

  • Page 73: Index

    Disposal of Sealed Lead Acid Batteries ....9 Trimming .......... 13, 29, 30, 50 Electrical Safety ............9 Vacutome ......14, 18, 29, 34, 59, 61 Environment ............9 Warnings ............5, 9 Favorites ............... 14 Warranty Statement ..........9 NX50 73 | Thermo Scientific...
  • Page 74 Appendices Waste Tray ............18 WEEE Directive ............ 9 74 | Thermo Scientific NX50...
  • Page 75 Index NX50 75 | Thermo Scientific...

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