Lowering Objective When Moving The Stage; Scan Overview Image - Zeiss ELYRA 7 Operating Manual

ZEISS
5.2.21.3

Lowering Objective when Moving the Stage

Some acquisition experiments require the objective lens to be lowered (or raised depending on the
microscope) before moving the scanning stage to the next position (e.g. required if certain plate formats
are used).
With the tool Positions, this is possible when activating
This feature is available to Position List and Sample Carrier if Show all is active.
Fig. 241 Scan panel
146
CHAPTER 1 - SYSTEM OPERATION
Left Tool Area and Hardware Control Tools
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5.2.21.4

Scan Overview Image

Pressing the Scan overview Image... button
opens the Scan overview Image interactive
window (Fig. 241). Here you can define the
number of Tiles by using the Horizontal and
Vertical Input boxes (the extension of the scanned
area will be displayed in Pixel and Size (µm)
units), the objective from the Objective drop
down menu and a potential zoom factor via the
Zoom slider or Zoom input box with which to scan
an overview image. For widefield modes, only the
zoomed in part of the camera chip will be
displayed. Changing Objective and Zoom will
change the size, but leaves Pixels unchanged. This
overview image can then be used to navigate in
the sample (i.e. define positions for imaging). Scan
starts the tile scan. Cancel closes the window
without further action.
ELYRA 7
check box.
03/2019 V_02