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Center Screen Area / Image Containers - Display and Image Analysis
6.10.2
Quantitative Colocalization Parameters Shown in the Data Table
No. of pixels in image ROI or scatter region
−
Area / relative area of image ROI or scatter region
−
Mean intensities / SD within image ROI or scatter region
−
Colocalization coefficients
−
Weighted colocalization coefficients
−
Overlap coefficient after Manders
−
Correlation coefficients (R and R
−
6.10.3
Colocalization Coefficients
pixels
Ch
, 1
coloc
c
=
1
pixels
Ch
total
, 1
Relative number of colocalizing pixels in channel 1 or 2, respectively, as compared to the total
−
number of pixels above threshold.
Value range 0 – 1 (0: no colocalization, 1: all pixels colocalize)
−
All pixels above background count
−
Weighted colocalization coefficients
∑
Ch
1
i,coloc
M
=
i
∑
1
Ch
1
i,total
i
Sum of intensities of colocalizing pixels in channel 1 or 2, respectively, as compared to the overall
−
sum of pixel intensities above threshold and in this channel.
Value range 0 – 1 (0: no colocalization, 1: all pixels colocalize)
−
Bright pixels contribute more than faint pixels
−
Correlation coefficient, Pearson´s correlation coefficient
∑
Ch
Ch
(
1
−
i
R
=
i
p
∑
(
Ch
1
−
Ch
1
i
i
Provides information on the intensity distribution within the colocalizing region
−
Value range -1 to +1
−
-1,+1: all pixels are found on straight line in the scatter diagram
0:
pixels in scattergram distribute in a cloud with no preferential direction
332
CHAPTER 1 - SYSTEM OPERATION
2
)
pixels
Ch
, 2
coloc
c
=
2
pixels
Ch
total
, 2
irrespective of their intensity.
∑
Ch
2
i,coloc
M
=
i
∑
2
Ch
2
i
Ch
Ch
1
)
( *
2
−
2
aver
i
aver
∑
2
)
*
(
Ch
2
−
Ch
2
aver
i
i
000000-2262-999
i,total
)
2
)
aver
ELYRA 7
03/2019 V_02
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