Simultaneous Observation In Phase Contrast + Fluorescence - Optika B-1000 Series Instruction Manual

15. Simultaneous observation in Phase Contrast + Fluorescence

• This microscope allows observation in transmitted light Phase contrast in combination with reflected light
Fluorescence. Samples with rapid decay must first be observed in Fluorescence and then in Phase Contrast.
The combined observation allows you to easily identify some areas of the sample that emit fluorescence.
1. Turn on the reflected light intensity dial.
2. Move the filter selector to an empty position or, if the filter holder is complete, to the position containing the UV filter
3. Insert the desired PH objective into the light path and insert the corresponding position of the phase contrast slider.
4. Focus the specimen.
5. Adjust the transmitted light intensity.
6. Move the filter cube selector to the desired filter position.
7. Adjust the reflected light intensity.
8. To obtain the proper specimen observation, adjust the transmitted light intensity, in order to modulate the fluorescence
intensity with the phase contrast intensity.
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