Use Of Oil Immersion Objective - Optika B-1000 Series Instruction Manual

10.15 Use of oil immersion objective

1. Focus the specimen with a low power objective.
2. Lower the stage (remember to lock the coarse upper limit
knob).
3. Put a drop of oil (provided) on the area of the specimen to be
observed. (Fig. 34)
• Make sure that there are no oil bubbles. Air bubbles in
the oil damage the image quality.
• To check for bubbles: remove an eyepiece, fully open the ap-
erture diaphragm and observe the objective exit pupil. (The
pupil must be round and bright).
• To remove the bubbles, gently move the nosepiece to the
right and to the left to move the immersion objective a few
times and allow the air bubbles to move away.
4. Insert immersion objective in the light path.
5. Return the stage to the upper focusing point and obtain an
optimal focus using the fine focus knob.
6. After use, gently remove the oil with a soft paper towel or a
lightly moistened optic paper with a mixture of ethyl ether
(70%) and absolute alcohol (30%).
• Immersion oil, if not immediately cleaned, could crystal-
lize creating a glass-like layer. In this situation the obser-
vation of the specimen would be difficult if not impossi-
ble due to the presence of an additional thickness on the
objective.
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F ig. 34 F ig. 34