Use Of The Microscope In Fluorescence (Reflected Light); Led Switch On; Use Of Fluorescence - Optika B-1000 Series Instruction Manual

12. Use of the microscope in Fluorescence (reflected light)

12.1 LED switch on

1. Turn the main switch ①. (Fig. 38)
2. Adjust to the desired brightness by rotating the dial ①.
12.2 Use of fluorescence
The filter turret is provided with 4 positions.
• In each of the four positions a fluorescence filter can be in-
serted, which can be selected from the options shown in the
table below.
• You can always add or replace an additional filter after
the first installation (see section 8.1.2 and 8.1.3).
• If all four turret positions are full, the observation in
transmitted light will be affected by the presence of the
fluorescence filter.
1. Move the filter selector ② in the desired position. (Fig. 39)
2. When the filter is in the click stop position, the dedicated LED
will turn on.
• When switching the fluorescence filter, the LED light
goes out. This is not a defect.
F I L T E R EXCITATION
NAME FILTER
M-1223 325-375 nm
M-1223.1 340 - 390 nm
M-1222 390-420 nm
M-1220 455-495 nm
M-1220.1 455-495 nm
M-1221 510-550 nm
M-1221.1 510-550 nm
M-1228 582-603 nm
M-1224 590-650 nm
M-1225 595-645 nm
M-1226 623-678 nm
M-1227 720-760 nm
D I C H R O I C BARRIER
MIRROR FILTER
415 nm 435LP nm
405 nm 420 - 470 nm
440 nm 450LP nm
500 nm 510LP nm
500 nm 518 - 542 nm
570 nm 575LP nm
570 nm 585 - 625 nm
610 nm 615-645 nm
660 nm 665LP nm
655 nm 665-715 nm
685 nm 690-750 nm
770 nm 780LP nm
②
• DAPI
• Hoechst
• DAPI
• Hoechst
• Coumarin
• Pacific Blue
• FITC
• GFP
• FITC
• GFP
• Rhodamine
• TRITC
• Propidium Iodide
• Rhodamine
• TRITC
• Propidium Iodide
• Red#2 FISH
• Cy5
• Cy5
• AlexaFluor 647
• Draq5
• Cy5.5
• Li_Cor IR dye 800
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①
APPLICATIONS
F ig. 38 F ig. 38
F ig. 39 F ig. 39