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Summary of Contents for Labnet BioPette A Series
- Page 1 Labnet BioPette A Single-channel, 8-channel, and 12-channel Pipettors Instruction Manual This manual is available in additional languages at www.labnetlink.com.
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Page 2: Table Of Contents
Table of Contents 1. Product Description ............. 1 2. Packing ..................2 3. Pipettor Design ................ 2 4. Safety Recommendations ..........3 5. Specifications ................4 6. Pipettor Operation ..............6 7. Operating Instructions ............10 8. Aspiration and Dispensing Instructions .....11 9. -
Page 3: Product Description
1. Product Description The Labnet BioPette A single-channel pipettor is a volumetric instrument designed to measure and transfer liquids precisely and safely. It is available in volumes from 0.1 μL to 10,000 μL. The Labnet BioPette A 8-channel and 12-channel pipettors are designed for microplate filling. The pipettors enable... -
Page 4: Packing
The 5,000 and 10,000 μL pipettors include a replaceable filter, fitted at the bottom of the shaft which prevents aspirated liquid from entering the shaft and contaminating the inner surface and plunger. Use of the filter is particularly important when aspirating and dispensing large volumes of liquid. The filter should be replaced if it becomes wet. 3. Pipettor Design Single- and Multi-channel Pipettors 2 – LABNET BIOPETTE A PIPETTORS... -
Page 5: Safety Recommendations
A. Pipetting pushbutton: Consists of 2 parts: the pushbutton (A1) and the knob (A2). B. Volume adjustment knob: Used to set the volume. C. Shaft: Manufactured using high quality plastic, ensuring high chemical and mechanical strength. D. Tip ejector: For multi-channel pipettors, the tips are ejected sequentially, reducing the required force. -
Page 6: Specifications
(repeatability) of the liquid volume depends on the quality of pipet tips used. The values for accuracy and precision shown in the table below were obtained using manufacturer non-filter pipet tips. Those tips are recommended for use to ensure compatibility, accuracy and precision when pipetting. 4 – LABNET BIOPETTE A PIPETTORS... - Page 7 Nominal Volume Volume Accuracy Precision Non-filter (µL) (µL) Tips (µL) Single-channel Pipettors Min. ±40.0 ≤12.0 ±12.0 ≤6.0 ±2.7 ≤1.3 Max. ±1.5 ≤0.7 Min. ±4.0 ≤2.8 ±2.5 ≤1.8 ±1.0 ≤0.6 Max. 10.0 ±0.5 ≤0.4 Min. ±3.0 ≤1.5 ±1.0 ≤0.5 Max. ±0.8 ≤0.3 Min.
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Page 8: Pipettor Operation
• Do not attach the tips with a rocking movement, as this may damage the shaft or plunger. Observe this rule particularly with single-channel pipettors of low volume range. • Never draw liquids directly into the pipettor without the tip attached. 6 – LABNET BIOPETTE A PIPETTORS... - Page 9 Adjusting the tip ejector The tip ejector can be adjusted by the user and can accommodate most types of tips available on the market. When using narrow tubes, it may be necessary to remove the tip ejector. 2-1,000 µL pipettors 1.
- Page 10 • Use the screwdriver to increase or decrease the ejector length. • Following ejector assembly or adjustment, ensure the ejector pushbutton is relocated. If the tip ejector cannot be adjusted sufficiently, or if the diameter of the ejector is insufficient to eject the tip, it may be necessary to attach the ejector cap “M” to the ejector. 8 – LABNET BIOPETTE A PIPETTORS...
- Page 11 In 2 and 10 μL pipettors place the cap, that can be purchased separately, on the bottom of the pipettor shaft and slide the cap upwards to the bottom of the tip ejector. Volume Setting Aspiration volume can be set by either turning the pipetting pushbutton knob, or the adjustment knob.
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Page 12: Operating Instructions
• Do not pipette liquids with temperatures above 70°C. NOTE: When pipetting acids or corrosive solutions which emit vapors, it is recommended to disassemble the shaft and rinse the plunger and O-ring with distilled water after finishing the pipetting operation. 10 – LABNET BIOPETTE A PIPETTORS... -
Page 13: Aspiration And Dispensing Instructions
8. Aspiration and Dispensing Instructions Aspirating Liquid 1. Press the pushbutton to the first stop point. Holding the pipettor vertically, immerse the pipet tip into the sample liquid to the recommended depth (for recommended values see Section 7). If the pipet tip is not immersed to the recommended depth or if the pushbutton is rapidly released, air may enter the pipet tip. - Page 14 Filters The 5,000 and 10,000 μL pipettors include a replaceable filter (L), fitted at the bottom of the shaft which prevents aspirated liquid from entering the shaft and contaminating the inner surface and plunger. Use of the filter is particularly important when aspirating and dispensing large volumes of liquid. The filter should be replaced if it becomes wet. 12 – LABNET BIOPETTE A PIPETTORS...
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Page 15: Checking Pipetting Accuracy Parameters And Pipettor Recalibration
9. Checking Pipetting Accuracy Parameters and Pipettor Recalibration The pipettors have been factory-calibrated using gravimetric methods with manufacturer pipet tips and distilled water, in accordance with ISO 8655 guidelines for the maximum (nominal) liquid volume drawn by the pipettor and for 10% of the maximum or minimum liquid volume according to the values given (Section 5). The pipettors are designed to enable recalibration and adaptation to different pipetting techniques and liquid properties (e.g., temperature, density, and viscosity). - Page 16 1.0035 1.0036 1.0037 1.0038 1.0038 1.0039 1.0040 1.0040 See ISO 8655 for the full table of conversion factors (Z). NOTE: Pipetting should be performed in accordance with guidance described in Sections 7 and 8. 14 – LABNET BIOPETTE A PIPETTORS...
- Page 17 Checking the Pipetting Accuracy Parameters • Set the preset volume depending on the pipettor volume according to the following table. Volume Change ΔV (μL) Nominal Preset for a Turn of a Calibration Permissible volume volume Key by values (µL) (µL) (µL) 1 Turn 1 Increment...
- Page 18 When pipetting liquids with physical properties considerably different from those of water, follow the guidance given in Section 5. More information on the calibration procedure can be found at www.labnetlink.com. 16 – LABNET BIOPETTE A PIPETTORS...
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Page 19: Pipettor Maintenance
10. Pipettor Maintenance Depending on the applications and intensity of use, the pipettor requires periodic maintenance. The components exposed to corrosive vapors, such as shaft elements, should be regularly checked and cleaned. Do not use sharp tools for pipettor maintenance. It may cause damage to the device and affect the user’s safety. -
Page 20: Troubleshooting
The shaft surface is Clean the shaft or drops out damaged or contaminated replace it with a new from the tip. at the sealing site. one. 18 – LABNET BIOPETTE A PIPETTORS... - Page 21 Problem Cause Solution The plunger or the O-ring is damaged due Disassemble the to prolonged aspiration shaft set; wash the of corrosive liquids. shaft, the plunger and The pipettor the seal (Section 10: incorrectly The inside of the pipettor Cleaning). Replace the aspirates is contaminated.
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Page 22: Spare Parts
Section 9. *Seal (J) used only in models 2, 5,000 and 10,000 μL Item Description Model (µL) Cat. No. Qty/Pk Pipetting pushbutton SP19337 SP19338 SP19339 SP19340 SP19341 1,000 SP19342 5,000 SP19343 10,000 SP19344 SP19346 SP19348 20 – LABNET BIOPETTE A PIPETTORS... - Page 23 Item Description Model (µL) Cat. No. Qty/Pk C, I, Shaft with O-ring and SP19111 seal SP19112 SP19113 SP19114 SP19115 1,000 SP19116 5,000 SP19118 10,000 SP19119 Tip ejector 2, 10 SP19440 SP19441 SP19442 SP19443 1,000 SP29419 5,000 SP19452 10,000 SP19453 2 - 1,000 SP29313 Shaft nut Piston assembly with...
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Page 24: Limited Warranty
Corning will not be responsible for damage incurred by improper packaging. Corning may elect for onsite service for larger equipment. 22 – LABNET BIOPETTE A PIPETTORS... - Page 25 Some states do not allow limitation on the length of implied warranties or exclusion or limitation of incidental or consequential damages. This warranty gives you specific legal rights. You may have other rights which vary from state to state. No individual may accept for, or on behalf of Corning, any other obligation of liability, or extend the period of this warranty. For your reference, make a note of the model number, serial number, date of purchase, and supplier here.
- Page 26 For additional product or technical information, visit www.labnetlink.com or contact your local sales office. N O R T H A M E R I C A E U R O P E t 800.492.1110 CSEurope@corning.com t 978.442.2200 France t 0800 916 882 A S I A / PA C I F I C Germany Australia/New Zealand t 0800 101 1153...
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