Related Manuals for Labnet BioPette Plus
Summary of Contents for Labnet BioPette Plus
- Page 1 Labnet BioPette Plus Single-channel, 8-channel, and 12-channel Pipettors Instruction Manual This manual is available in additional languages at www.labnetlink.com.
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Page 2: Table Of Contents
Table of Contents 1. Product Description ............... 1 2. Packing ..................2 3. Pipettor Design .................2 4. Safety Recommendations ..........4 5. Specifications ................5 6. Pipettor Operation ..............7 7. Operating Instructions ............11 8. Aspiration and Dispensing Instructions .......12 9. Checking Pipetting Accuracy Parameters and Pipettor Recalibration ..........14 10. -
Page 3: Product Description
It is available in volumes from 0.1 μL to 10,000 μL. The BioPette Plus 8-channel and 12-channel pipettors are designed for microplate filling. The pipettors enable precise and simultaneous delivery of 8 or 12 preset volumes of liquid. -
Page 4: Packing
The filter should be replaced if it becomes wet. 3. Pipettor Design Model Identification The volume range of the pipettor is shown on the pushbutton and on the tip ejector bar in multi-channel pipettors. 2 – LABNET BIOPETTE PLUS PIPETTORS... - Page 5 Single- and Multi-channel Pipettors A. Pipetting pushbutton: Consists of 2 parts: the pushbutton (A1) and the knob (A2). B1. Volume adjustment knob: Used to set the volume. B2. Locking Ring: Allows the volume setting to be locked by pushing the ring upwards. C.
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Page 6: Safety Recommendations
• Take special care when pipetting aggressive substances. • Use appropriate protective attire (e.g., clothing, goggles, and gloves). • Avoid pointing the pipettor at yourself or others during use. • Only use parts and accessories recommended by the manufacturer. 4 – LABNET BIOPETTE PLUS PIPETTORS... -
Page 7: Specifications
5. Specifications The pipettor is a high quality instrument which offers excellent accuracy and precision. The accuracy and precision (repeatability) of the liquid volume depends on the quality of pipet tips used. The values for accuracy and precision shown in the table below were obtained using manufacturer non-filter pipet tips. - Page 8 The use of tips from other manufacturers or filter tips may result in incorrect liquid aspiration and require pipettor recalibration. The design of the pipettor enables the user to recalibrate it according to the information presented in Section 9. 6 – LABNET BIOPETTE PLUS PIPETTORS...
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Page 9: Pipettor Operation
6. Pipettor Operation Attaching the Tips • Attach the correct tip corresponding to the model number displayed on the pipettor pushbutton (Section 5). • Position the pipettor vertically when attaching tips. • Single-channel pipettors: push the pipet tip on firmly using a slight twisting motion to ensure an airtight seal. •... - Page 10 • Turn the tip ejector bush clockwise until it has fully latched. 3. Tip ejector adjustment • Remove the tip ejector pushbutton. • To increase the ejector length turn the plastic bush counter-clockwise. • To decrease the ejector length turn the plastic bush clockwise. 8 – LABNET BIOPETTE PLUS PIPETTORS...
- Page 11 5,000 and 10,000 μL pipettors 1. Tip ejector disassembly • Remove the tip ejector pushbutton. • Using a screwdriver, turn the metal arbor counter- clockwise to unscrew the ejector from the arbor. • Release the ejector. 2. Tip ejector assembly •...
- Page 12 The smallest volume increment is printed on the bottom counter drum. 3. After the volume has been set, set the locking ring to the upper position to avoid inadvertent changing of volume. 10 – LABNET BIOPETTE PLUS PIPETTORS...
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Page 13: Operating Instructions
Examples of counter indications The decimal point in the volume of aspirated liquid (µL or mL) is indicated by change in colour of digits. Black digits represent integer numbers, and red digits represent decimal fractions (sizes up to 20 µL). Volume Ranges 0.1-2 µL 5-50 µL... -
Page 14: Aspiration And Dispensing Instructions
(for recommended values see Section 7). If the pipet tip is not immersed to the recommended depth or if the pipetting pushbutton is rapidly released, air may enter the pipet tip. 12 – LABNET BIOPETTE PLUS PIPETTORS... - Page 15 2. Release the pipetting pushbutton slowly and smoothly to aspirate the sample. Wait one second and then withdraw the pipet tip from the liquid. CAUTION: Do not touch the used tip. Dispensing Liquid 3. Place the end of the pipet tip against the inside wall of the vessel at an angle of 10°...
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Page 16: Checking Pipetting Accuracy Parameters And Pipettor Recalibration
The recommended volume for recalibration is 10% of the maximum (nominal) value or minimum volume depending on which of these values is greater. See table on page 16 for more details. 14 – LABNET BIOPETTE PLUS PIPETTORS... - Page 17 Parameters for Checking the Pipetting Accuracy The pipetting accuracy is influenced by factors such as: tips used, characteristics of the pipetted liquid (density, viscosity), and operating conditions (ambient temperature, pressure). To determine the accuracy error of the pipettor, the following conditions should be met: •...
- Page 18 • Calculate the volume in [μL] by multiplying the value in [mg] by the conversion factor Z [μL/mg]. If the average aspirated volume exceeds the permissible values of the range, the pipettor should be recalibrated. 16 – LABNET BIOPETTE PLUS PIPETTORS...
- Page 19 Pipettor Recalibration KK K 1. Remove the pipetting pushbutton. CAUTION: The pipetting pushbutton consists of 2 parts: the knob and the pushbutton. After removal of the pushbutton, both parts are separated. 2. Holding the volume setting knob to prevent rotation, insert the calibration key into the calibration screw.
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Page 20: Pipettor Maintenance
• Sterilize the pipettors using an autoclave with an initial vacuum and drying cycle. • After sterilization, the pipettor should be dried and cooled to room temperature. • The shaft of the P3942-5000 and P3942-10000 models should be autoclaved without the filter. 18 – LABNET BIOPETTE PLUS PIPETTORS... -
Page 21: Troubleshooting
Precision and accuracy should not alter if the pipetting processes including autoclaving are carried out as described in this manual. If a change in accuracy occurs, it is recommended to: • Check the calibration of the pipettor after the first, third, and fifth autoclaving cycles and then after every 10 autoclaving cycles. - Page 22 If the problem persists after carrying out the above steps, contact our regional representative. Before returning the pipettor, please ensure the pipettor is completely free of any chemical, radioactive, or microbiological contamination which could pose a threat during transport and repair. 20 – LABNET BIOPETTE PLUS PIPETTORS...
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Page 23: Spare Parts
12. Spare Parts Contact our Customer Service to inquire about availability of spare parts. Pipettor model and name of the part required should be specified. The most common parts are depicted below. NOTE: The replacement of the plunger requires conducting the calibration procedure according to Section 9. - Page 24 5,000, 10,000 SP19476 Shaft filter Tip ejector cap 2, 10 SP19378 5,000 SP19466 10,000 SP19467 Color identification ring 2-1,000 SP19630 SP19451 Shelf clip *Seal (J) used only in models 2, 5,000 and 10,000 µL 22 – LABNET BIOPETTE PLUS PIPETTORS...
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Page 25: Limited Warranty
13. Limited Warranty Corning Incorporated (Corning) warrants that this product will be free from defects in material and workmanship for a period of three (3) years from date of purchase. CORNING DISCLAIMS ALL OTHER WARRANTIES WHETHER EXPRESSED OR IMPLIED, INCLUDING ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR OF FITNESS FOR A PARTICULAR PURPOSE. - Page 26 Not intended for use in diagnostic or therapeutic procedures. Corning makes no claims regarding the performance of these products for clinical or diagnostic applications. Product availability may vary per region. 24 – LABNET BIOPETTE PLUS PIPETTORS...
- Page 27 For additional product or technical information, visit www.labnetlink.com or contact your local sales office. N O R T H A M E R I C A E U R O P E t 800.492.1110 CSEurope@corning.com t 978.442.2200 France t 0800 916 882 A S I A / PA C I F I C Germany Australia/New Zealand...
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