Low-Pressure Gradient System - Hitachi CHROMASTER 5160 Instruction Manual

1.2 Low-Pressure Gradient System

Wash fluid
Organizer
Chromaster 5110
Pump
Degassing
unit
(4) Each component separated from the sample is then
detected as a signal by the detector.
In the low-pressure gradient system, two or more solvents are
mixed in its low-pressure section from which a liquid feed is
performed by a single pump. The composition of an eluent is
made to vary with time in chromatographic separation.
Mobile phase
Mixer
4-way joint
Proportioning valve
Fig. 1-2 Low Gradient System
(1) Each of plural eluents is fed from the degassing unit to the
low-pressure unit.
(2) In the low-pressure unit, the eluents are mixed at a pre-
specified ratio.
(3) Through the mixer, the mixture eluent is delivered.
(4) A sample is injected by the autosampler.
(5) The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
(6) Each component separated from the sample is detected as
a signal by the detector.
Colum
n
Syringe valve
1 - 2
Chromaster 5440
Fluorescence detector
Detector cell
Chromaster 5310
Column oven
Injection valve
Chromaster 5210
Autosampler
Wash pump
Drain