Hitachi CHROMASTER 5160 Instruction Manual page 808

(2) Wash fluid selection and use examples
The Chromaster 5210 autosampler uses two kinds of wash
fluid. Refer to the following table.
Table 2-2
One Fluid Wash <standard>
(for Cut injection method,
Full loop injection method)
Wash fluid 1 Sample solution
Mobile phase
Wash fluid 2 Aqueous solvent
Table 2-3
One Fluid Wash <standard>
(for Cut injection method,
Full loop injection method)
Wash fluid 1 Sample solution
Wash fluid 2 Aqueous solvent
*1) As a washing solution in cases where a solvent
*2) When the aqueous solvent is used for washing of a
Isocratic Analysis
*1)
,
*1)
*2)
Gradient Analysis
*1)
,
*2)
containing a buffer solution or salt has been fed as the
mobile phase, use a mobile-phase solvent containing no
salt.
(When 20 mmol/L KH2PO4:CH3CN＝50:50 is used for
the mobile phase, use H2O:CH3CN＝50:50.)
If a solvent containing salt is used for washing, it causes
damages, such as the surface of the glass, the tip of the
syringe, and the inside of the wash pump.
Additionally, it is resulting in deterioration of performance
or shorter service life.
plunger seal for the Chromaster 5110 pump.
For example, when an organic solvent is used as wash
fluid1 in the normal phase chromatography analysis
which the cut injection method is applied, salt
precipitation in the pump plunger can't be washed off
(Salt doesn't dissolve). In that case, use the aqueous
solvent as wash fluid 2.
2 - 12
Two Fluids Wash
(for All volume injection method,
Low carry over analysis)
*1)
Sample solution
(Strong wash)
*1)
Mobile phase
(Weak wash)
Two Fluid Wash
(for All volume injection method,
Low carry over analysis)
*1)
Sample solution
*3)
Gradient final solvent
(Strong wash)
Gradient initial solvent
(Weak wash)
*1)