Outline; Isocratic System - Hitachi CHROMASTER 5160 Instruction Manual

1.1 Isocratic System

Wash fluid
Degassing
unit

1. OUTLINE

The liquid chromatograph system consists of a pump for feeding
a mobile phase, a sample introducing section (autosampler,
manual injector) for injecting a sample, a column for
chromatographic separation of the injected sample, a column
oven for maintaining the column at a constant temperature, and
a detector section for detecting a signal of a separated
component of the sample.
In addition, a degassing unit for deaerating an eluent, a gradient
device for varying a mixing ratio of eluents with time, and other
optional devices are incorporated in the system.
The isocratic system has a simple configuration in which single-
solvent analysis is performed.
Mobile phase
Syringe valve
Fig. 1-1 Isocratic System
(1) An eluent is fed by the pump through the degassing unit.
(2) A sample is injected from the autosampler.
(3) The injected sample is separated through the column which
is maintained at a constant temperature by the column oven.
1 - 1
Chromaster 5440 Fluorescence
detector
Column
Drain
Detector cell
Chromaster 5310 Column
oven
Injection valve
Chromaster 5210
Autosampler
Wash pump