tecan GENios Operating Manual page 7

7. Quality Control
7.1
7.2
7.2.1
7.2.2
7.2.3
7.2.4
7.3
7.3.1
7.3.2
8. Cleaning & Maintenance
8.1
8.2
8.3
8.4
8.4.1
8.4.2
8.4.3
8.4.4
8.5
8.5.1
8.5.2
8.6
9. Application Note for DNA Quantification
9.1
9.1.1
9.2
9.2.1
9.2.2
9.3
9.3.1
9.3.2
9.3.3
9.3.4
9.4
Index
June 2002 Operating Manual for GENios, GENios FL, and GENios Plus No: I 112 904 Rev No: 1.1
Periodic Quality Control Tests ..................................................... 7-1
Fluorescence ................................................................................. 7-1
Sensitivity Test ................................................................................ 7-1
Linearity Test ................................................................................... 7-2
Precision Test.................................................................................. 7-3
Cross-Talk Test ............................................................................... 7-3
Glow Type Luminescence ............................................................ 7-4
Sensitivity Test ................................................................................ 7-4
Cross-Talk Test ............................................................................... 7-5
Introduction.................................................................................... 8-1
Liquid Spills ................................................................................... 8-1
Replacing the Fuse........................................................................ 8-2
Maintenance................................................................................... 8-3
Daily................................................................................................. 8-3
Weekly............................................................................................. 8-3
Every Six Months............................................................................. 8-3
Yearly Maintenance......................................................................... 8-3
Instrument Disinfection ................................................................ 8-4
Disinfection Solutions ...................................................................... 8-4
Disinfection Procedure .................................................................... 8-5
Disinfection Certificate ................................................................. 8-6
Introduction.................................................................................... 9-1
Common techniques for the quantitation of DNA ............................ 9-1
Experimental Setup ....................................................................... 9-2
DNA extraction ................................................................................ 9-2
Assay Procedure ............................................................................. 9-2
Typical Results .............................................................................. 9-3
Detection techniques vs detection limits.......................................... 9-3
Sensitivities in comparison: ............................................................. 9-4
Results from Plasmid and Blood DNA Sample................................ 9-4
DNA Purity at 260/280 nm ............................................................... 9-5
Conclusion ..................................................................................... 9-5
Acknowledgements ......................................................................... 9-5
7