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8.3
Inserting the knife or blade in the knife
holder
All components of the cryostat as well as the
knife or blade and the tools for specimen
preparation should be precooled in the cry-
ostat before starting sectioning!
• Insert the precooled knife or blade in the knife
holder and clamp.
For further details, please refer to the separate instruc-
tion manual for your knife holder.
Take care when handling microtome knives
and disposable blades. The cutting edge is
extremely sharp and can cause severe injury!
Never leave knives/blades or knife holders
with a knife/blade mounted lying around!
Do not place a knife on a table with the cut-
ting edge facing upward!
Never try to catch a falling knife!
Always clamp the specimen before the knife!
Prior to manipulating the knife and specimen,
or changing the specimen or knife, and dur-
ing breaks, always lock the handwheel and
cover the cutting edge with the knife guard!
Avoid skin contact with cold parts of the in-
strument as this can cause frostbite!
Leica CM1510 – Cryostat
8.4
Moving the specimen towards or away
from the knife/blade via the coarse feed
Always observe the specimen during the rapid
forward movement towards the knife/blade,
to avoid that the specimen collides with the
cutting edge. A collision can cause severe
damage both to the knife or blade and the
specimen!
• Push the coarse feed button to approach the speci-
men to the cutting edge.
Fig. 19
8.5
Trimming
• Remove the knife guard from the cutting edge.
• Remove the anti-roll guide from the knife.
• Unlock the handwheel.
• Rotate the handwheel cautiously and check if the
specimen comes in contact with the cutting edge
of the knife.
• To trim the specimen, select a section thickness of
approximately 30 µm with the section thickness
selection knob on the front of the microtome.
• Trim the specimen to the desired sectioning plane
by gradually decreasing the section thickness.
8. Daily operation
away from the knife
towards the knife
19
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