Optional Accessories; Heat Extractors; Thermal Block - Leica CM1510 Instruction Manual

Cryostat
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12.

Optional accessories

12.1 Heat extractors

Specimen freezing on the quick-freeze shelf can be ac-
celerated by the additional use of a heat extractor.
12.1.1 Mobile heat extractor
Fig. 30.1
• Store the heat extractor in the cryochamber.
• Place it on the specimen surface to accelerate
freezing.
• Remove it once the specimen is entirely frozen.
12.1.2 Stat ionary heat extractor
Fig. 30.2
• Screw the heat extractor into the hole on the quick-
freeze shelf. It can reach three specimen discs.
• Lower the heat extractor cylinder on the tissue sur-
face. After approximately 30 seconds, the specimen
will be entirely frozen.
To prevent unnecessary distortion of the
specimen during contact freezing, the weight
of the heat extractor can be balanced.
• To balance the weight of the cylinder, loosen screw
(2) and move the knob (1) up or down as required.
• Retighten screw (2) to fix the knob (1) in the appro-
priate position.
30

12.2 Thermal block

The thermal block (8) facilitates the removal of the fro-
zen specimen from the specimen disc.
Keep the thermal block outside the cryocham-
ber at room temperature.
5
6
7
8
9
Fig. 30.3
• Place the cap (9) on the required side, so that the
appropriate location hole for the specimen disc is
visible.
• Insert the shaft (6) of the specimen disc (5) in the
appropriate location hole (7) at the top or bottom of
the thermal block.
• After about 20 seconds, the frozen specimen can
be removed from the specimen disc with forceps.
• If the cap is too loose, readjust it with the small
screw (10). Do not overtighten the screw.
• Once the specimen is removed, take the thermal
block out of the cold cryochamber.
10
Instruction Manual V2.1 – 12/2001

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