IKA HABITAT Manual
  1. Manuals
  2. Brands
  3. IKA Manuals
  4. Laboratory Equipment
  5. HABITAT
  6. Manual

IKA HABITAT Manual

Hide thumbs Also See for HABITAT:
Table of Contents

Advertisement

Quick Links

Download this manual
HABITAT cell
HABITAT photo cell
HABITAT cell cct
HABITAT photo cell cct
HABITAT ferment
HABITAT photo ferment
HABITAT ferment cct
HABITAT photo ferment cct
HABITAT cell vessel
0.5 L sw
0.5 L dw
1 L sw
1 L dw
2 L sw
2 L dw
5 L sw
5 L dw
10 L dw
3.2 L su sw
HABITAT ferment vessel
0.5 L dw
1 L dw
2 L sw
2 L dw
5 L sw
5 L dw
10 L dw

Advertisement

Table of Contents
loading

Related Manuals for IKA HABITAT

Summary of Contents for IKA HABITAT

  • Page 1 HABITAT cell vessel HABITAT cell HABITAT photo cell 0.5 L sw HABITAT cell cct 0.5 L dw 1 L sw HABITAT photo cell cct 1 L dw 2 L sw 2 L dw 5 L sw 5 L dw 10 L dw 3.2 L su sw...
  • Page 2 ENGLISH...

  • Page 3: Table Of Contents

    EU Declaration of conformity .............6 Explication of warning symbols ............6 Safety instructions ................7 Intended use ................... 10 Unpacking ..................10 Useful information ................13 Installation ..................14 Operation ..................32 Interfaces and outputs ..............77 Fig. 1 Maintenance and cleaning .............. 82 Accessories ..................

  • Page 4: Eu Declaration Of Conformity

    A copy of the complete EU Declaration of Conformity or further declarations of conformity can HABITAT operating instructions it is also available as PDF or to be downloaded from the UI. › Ensure that only trained staff work with the device.
  • Page 5 › It may be possible for wear debris from rotating accessory parts to reach the material being › Restart PC / App and the IKA Biolab software if a software failure occurs or automatically processed.

  • Page 6: Intended Use

    HA.sp.m.x (Micro sparger) or HA.sp.r.x* (Ring sparger) HA.cab.sw (cable set for single wall) or HA.cab.dw HA.cn (Condenser) (cable set for double wall) HABITAT cell / ferment Power cord set H 11, 2x HA.ip.pi.x (Impeller 3-pitch blade) or HA.ip.b.x* HA.sf.250 (Bottle 250 ml x4)

  • Page 7: Useful Information

    HA.s.do.2 (DO sensor) HA.sf.250 (Bottle 250 ml x4) Useful information HA.cab.dw › The HABITAT is operated by an Android or the setting before mode entry. iOs tablet PC, which is part of the delivery › Mains switch: Power-ON / Power-OFF the scope.

  • Page 8: Installation

    Installation /// Vessel /// Gas Single-wall glass vessel Outlet Max. 1.0 barg Inlet Barb 5 mm 0.5 L Max. 3.0 barg 10 L Tube OD 6 mm Double-wall glass vessel /// Interfaces 0.5 L save data Single-use plastic vessel 3.2 L communication with GUI software RS-232...
  • Page 9 /// Lid with plugs and fittings 0.5 L /// Drive shaft 10 L Allen key or rod 4 mm /// Impeller ≥ 20 mm...
  • Page 10 /// Harvest pipe /// Baffle /// Ring or micro sparger /// Condenser...
  • Page 11 /// Level sensor /// Temperature sensor /// Vessel and stand /// Lid and vessel /// DO, pH, CO , conductivity or turbidity probe Caution! Notice! › Read the operating instructions for the pro- › Related on the lid size the ports may vary! bes carefully!
  • Page 12 /// Peltier cooling /// Foam sensor › Blue LED ON: Good alignment between the sensor boxes, and no foam in between. › Blue LED OFF: Bad position installed for the sensor boxes, or with obstruction in between. If not in use /// Motor /// Heat blanket Warning!
  • Page 13 /// Positioning /// Pump tubing › Tube flow rate Warning! › For autoclave, please remove the motor and keep the connectors of double-wall glass vessel open! Flow rate (ccm / min) Tube ID Pump head adjustment 0.5 mm First position 0.8 mm First position 1.2 mm...
  • Page 14 /// Hose connecting › Tubing filling 4. Input "Tubing length" in cm 1. Before the calibration, select pump "Filling" 5. Start "Filling" 2. Click "Filling" 6. Wait until "Filling" completed 3. Choose "Tubing ID" Flask stand (HA.sfr.250, accessories)
  • Page 15 Overlay Heat blanket Filter heater DO sensor 4 gases system / IKA HABITAT Cell growth single use 4 gases system / IKA HABITAT Cell growth & Ferment * Accessories * Accessories 1 bar ** Cell growth 0...2000 ccm Ferment 0...20000 ccm...
  • Page 16 /// Lid stand (Accessories) /// SU vessel and SU vessel holder While in use HA.ls.10 HA.ls.2 Motor SU Vessel (with / without integrated single-use sensors) SU Vessel Holder Note: When using the SU vessel with the integrated single-use sensors, the calibration data While not in use have to be entered and the sensors calibrated before starting the process.

  • Page 17: Operation

    Safe-stop User enabled 6. When the medium is in good condition, feed the cell and nutrient into the vessel. User role Monitor 7. Start "Cultivation", and let HABITAT automatically control all environment following the user Process view Operator setting. Admin...
  • Page 18 Process view Batch Batch ID On delay information On time Name Sensitivity Recipe Level limit Mode Status Auto Elapsed Time On delay Note On time Start Button Start Cultivation Entering batch name Sensitivity Entering note / comment Level control Harvest Choose recipe mode Feed...
  • Page 19 Setup view General Batch mode Batch Select output Overlay path for Air Sparger Fed-batch Overlay & Sparger Perfusion Select output Overlay Select logging 5 sec path for O Sparger interval 10 sec Overlay & Sparger 30 sec Select output Overlay 1 min path for N Sparger...
  • Page 20 Trend view Trend settings Enable Alarm Timestamp Plot Parameter Stirrer Error code Temp Description Value Acid Base Mantenance Sensor Temp Last Calibration Anti-foam Calibration Serial no. Level Zero Slope Condition Action Light Last Calibration probe Serial no. Zero Turbid Slope Plot Color Condition Action...
  • Page 21 Device type Not set HABITAT Cell HABITAT Ferment HABITAT Cell Photo HABITAT Ferment Photo HABITAT Cell CCT HABITAT Ferment CCT HABITAT Cell Photo CCT HABITAT Ferment Photo CCT Logging level Info Debug Log file to keep (days) Info About Device information...
  • Page 22 1.2 Screen Layout Mobile Click on the plus sign (+) to open menu for re-scanning of new devices or entering the infor- mation to connect the device manually if it’s not discovered by the software. If the device is turned on, but not discovered by the software, the user is able to enter the information manually to connect the device.
  • Page 23 2.2 Connecting to device screen 2.3 Application Header Menu Software will establish connection to the device and sync all required data from the device. 2.3.1 Header TAB Button During data sync operation the connection or data sync maybe failing due to not stable Using to navigate to the screens.
  • Page 24 2.5 Process view 2.5.1 Batch information panel Start screen with schematic illustration of the controlled culture vessel: The panel shows information for the current / last batch i.e. ID, › Display of components of the current configuration Name, and Recipe in use, status, Elapsed time and Note that user ›...
  • Page 25 2.5.3 Stirrer Control 2.5.4 Gas Control (Air, O2, N2, CO2) Mode: Mode: › Off: Turn off the stirrer › Off: Turn off the gas module › Manual: Set speed of the stirrer manually (freely), not depending on other parameter. › Manual: Set the gas flow rate manually (freely), not depending on other parameter. ›...
  • Page 26 2.5.5 Gas Mixer (Air, O2) › Gas Mixer: Set Air and O2 control mode to Manual mode. › If the Gas Mixer is enabled the set point input from Air and O2 control will be disabled. › Set flow: Entering flow rate in vvm (Volume Vessel per Minute), up to 2 vvm. ›...
  • Page 27 2.5.8 Foam limit Control 2.5.10 Temperature Control Mode: Mode: › Off: The sensor will detect the foam level but not take any action. › Off: Turn off › Auto: If foam is detected the controller will control the Anti-foam pump to fill in Anti-foam. ›...
  • Page 28 2.5.12 pO2 Control 2.6 Setup (Recipe) Menu Mode: To create, save and restore the setup: › Off: Turn off › Re-use previous configuration of the component use in the experiment. › Manual: To control the output power (%) of the pO2 controller directly with Cascade control ›...
  • Page 29 2.6.2 Reactor 2.6.3 Pumps 1 Vessel type and size The control tower consist of 4 built-in pumps called Pump A (top) to Pump D (bottom). (To effect min/max limits of parameter such as Gas flow rate and vessel visualization) It is also possible to add an external pump called Ext A. 2 Sparger type (for report only) 1 To enable / disable the pump.
  • Page 30 2.6.4 Gases 2.6.5 Controllers 1 To enable / disable up to 4 gas modules for using in the experiment. pO2 controller 2 To enable / disable the Gas Mixer (Air, O2). When the Gas mixer is enabled the flow rate of 1 Enable / Disable parameter use to control pO2 value Air and O2 is controlled by the Gas mixer.
  • Page 31 pH controller 2.6.6 Cell & Medium information 1 Enable / Disable parameter to control pH value 1 To fill in cell / product information 2 Select output path 2 To fill in culture medium information 3 To fill in supplement information...
  • Page 32 2.6.7 Accessories & Option 2.7 List View Menu 1 To enable / disable Light panel The screen display overall parameter in the table: 2 To enable / disable CO2 sensor › Actual value 3 To enable / disable Conductivity sensor ›...
  • Page 33 2.8 Trend View Menu 1 To enable / disable Trend plotting channel To plot real-time data to the graph. 2 To choose parameter to plot The plot data is refreshed every time when the GUI is connected to the device or the device 3 To choose plotting color time is changed.
  • Page 34 2.9 Historical data 2.10 Alarm view 1 Table to display all historical experiment from the device The screen will display alarm for the active running experiment. If the experiment is not running 2 Historical Graph plot area to review data the alarm will not be recorded.
  • Page 35 2.11 Maintenance Menu 2.11.2 Pump Calibration 1 Latest date and time that calibration has been conducted. 2.11.1 Sensor Calibration 1 Latest date and time that calibration has been conducted. 2 Tubing inner diameter. 2 Probe serial number. 3 Set value that used to calibrate pump in the last calibration. 3 Raw probe value when doing zero calibration.
  • Page 36 2.11.3 Controller 2.12 Settings Menu The screen is mostly for the experienced user with knowledge of a Control system to do PID 2.12.1 General controller tuning. For mostly of the user there is no need to adjust any parameters on the 1 To change the device name.
  • Page 37 The menu allows to change the system parameter, delete or restore configuration which may not be compatible with the device! Only admin level user can access some menu on the screen. However some menu will need IKA services with service level password to access and change the parameter. 1 Restore factory default configuration.
  • Page 38 3 Manual: Open HABITAT operating instructions. › After autoclaving: Be aware of the hot surfaces! 4 Video Users guides: Link tutorial videos YouTube playlist. 5 Shop: Link to HABITAT product page on the IKA website. Caution! › Check that the vessel fits the autoclave device! ›...

  • Page 39: Interfaces And Outputs

    1.5 bar) and adjust the flow rate slowly. https://www.ika.com/ika/lws/download/usb-driver.zip Install the driver by running the setup file. Connect the IKA device through the USB data cable to the PC. The data communication runs through a virtual COM port. /// Sampling Note: The USB driver is uncessary for Windows 10 system.
  • Page 40 1.1). The NAMUR commands and the additional specific IKA commands commissioning serve only as low level commands for communication between the device and the PC. With a suitable ter- minal or communications program these commands can be transmitted directly to the device.
  • Page 41 › Ethernet + WiFi › IKA Cloud Ethernet + WiFi IKA Cloud GUI (Windows) Ethernet WiFi Router IKA Cloud Router & hub Internet Ethernet (Android App / iOS App) Internet Ethernet › Remote Remote Router & hub Internet Ethernet Internet...

  • Page 42: Maintenance And Cleaning

    /// Cleaning › For cleaning disconnect the mains plug! /// Maintenance Checklist › Use only cleaning agents which have been approved by IKA to clean IKA devices: Water containing surfactant / isopropyl alcohol. › Wear protective gloves during cleaning the devices.

  • Page 43: Error Codes

    Error codes Error Error message Causes Effect Solutions code 01011315 Air Flow disconnected › The fault is shown by an error code on the display as following if the error occurs. Proceed as follows in such cases: Carbon dioxide (CO ) Flow 01021315 disconnected...

  • Page 44: Technical Data

    Technical data Pump Module Quantity 4 PC Controller Variable speed (CW / CCW) Environment ID 0.5 mm: 1 ... 4 ccm/min Permissible ambient temperature + 5 ... + 40 °C ID 0.8 mm: 3 ... 12 ccm/min Permissible relative humidity ID 1.2 mm: 5 ...
  • Page 45 Overall dimension (mm) Vessel information Vessel size Width Height Depth Glass Glass Glass Glass Glass Unit vessel vessel vessel vessel vessel 3.0 L Glass vessel 0.5 L 0.5 L 10 L Glass vessel 1L Total volume 3.00 12.9 Glass vessel 2L Max.
  • Page 46 Space requirement for autoclaving (mm) Multiuse vessel Vessel size Width Height Depth Material Glass vessel 0.5 L Vessel Borosilicate glass 3.3 Glass vessel 1L Components contact to medium Stainless steel 316 Glass vessel 2L Components not contact to medium Stainless steel 304 Glass vessel 5L Gasket EPDM...
  • Page 47 Single use vessel SU holder integrated with pressure relief unit Material SU Holder Standard Vessel & components Polycarbonate Dimensions (W x L x H) 260 x 260 x 100 mm Tubing Silicone USP class VI Weight 10.8 kg O-ring Silicone USP class VI Pressure relief unit 0.5 bar Vessel dimension...
  • Page 48 Warranty PC requirements Minimum Recommended › In accordance with IKA warranty conditions, the warranty period is 24 months. For claims Operating system Windows 10, 32/64 bits Windows 10, 64 bits under the warranty please contact your local dealer. You may also send the device direct to Intel ®...
  • Page 49 Phone: +84 28 38202142 Phone: +66 2059 4690 Phone: +90 216 394 43 43 eMail: sales.lab-vietnam@ika.com eMail: sales.lab-thailand@ika.com eMail: sales.turkey@ika.com Discover and order the fascinating products of IKA online: www.ika.com IKAworldwide IKAworldwide /// #lookattheblue @IKAworldwide Technical specifications may be changed without prior notice.

Table of Contents