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IKA HABITAT cell Manual
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IKA HABITAT cell Manual

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HABITAT cell
HABITAT photo cell
HABITAT cell cct
HABITAT photo cell cct
HABITAT ferment
HABITAT photo ferment
HABITAT ferment cct
HABITAT photo ferment cct
HABITAT cell vessel
0.5 L sw
0.5 L dw
1 L sw
1 L dw
2 L sw
2 L dw
5 L sw
5 L dw
10 L dw
HABITAT ferment vessel
0.5 L dw
1 L dw
2 L sw
2 L dw
5 L sw
5 L dw
10 L dw

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Summary of Contents for IKA HABITAT cell

  • Page 1 HABITAT cell vessel HABITAT cell HABITAT photo cell 0.5 L sw HABITAT cell cct 0.5 L dw 1 L sw HABITAT photo cell cct 1 L dw 2 L sw 2 L dw 5 L sw 5 L dw 10 L dw...
  • Page 2 ENGLISH...

  • Page 3: Table Of Contents

    EU Declaration of conformity .............6 Explication of warning symbols ............6 Safety instructions ................7 Intended use ................... 10 Unpacking ..................11 Useful information ................15 Installation ..................16 Operation ..................34 Interfaces and outputs ..............104 Fig. 1 Maintenance and cleaning ............108 Accessories ...................

  • Page 4: Eu Declaration Of Conformity

    Directive: 2014/53/EU Standards: EN 60950-1, EN 300328, EN 301489-1, EN 301489-17 interface and also from the IKA homepage. A copy of the complete EU Declaration of Conformity or further declarations of conformity can › Ensure that only trained staff work with the device.
  • Page 5 Warning! › Safe operation is guaranteed only with the use of original IKA accessories. › Only process media that will not react dangerously to the extra energy produced through › Ensure that the external temperature sensor is inserted into the medium to a depth of at processing.

  • Page 6: Intended Use

    › After a power failure during operation, the device may start automatically (depending on the operating mode). › Restart PC / App and the IKA HABITAT software if a software failure occurs. IKA HABITAT will › Unpack the device carefully. Any damage should immediately be reported to the carrier (mail, run without an active connection with PC / App.
  • Page 7 HA.vh.x vessel holder incl. HA.lc.x lid HA.s.ph.x (pH sensor) mounted HA.s.do.x (DO sensor) HA.gv.sw.x (vessel single wall) or HA.gv.dw HA.s.fo (Foam sensor) For ferment only For SW 2&5 L ferment only (vessel double wall) HA.hbl.x (Heat blanket) HA.mt.s.x (Motor) HA.cab.sw (cable set for single wall) or HA.sp.m.x (Micro sparger) or HA.sp.r.x* (Ring HA.cab.dw (cable set for double wall) sparger)

  • Page 8: Useful Information

    Useful information HA.cab.sw › The HABITAT is operated with an iOS Tablet PC (included in the scope of delivery). › Alternatively, the operation is also available via an MS-Windows PC / notebook. ® › Wired and wireless operation is supported. Monitoring is also available for an iOS mobile system by means of the Bioreactor App.

  • Page 9: Installation

    Installation /// Interfaces /// Gas Max. 1.0 barg Barb 5 mm Outlet Inlet Max. 3.0 barg Tube OD 6 mm USB connection (data storage) PC connection (communication with GUI software) RS-232 RS 232 connection (communication with GUI software) ETHERNET Ethernet connection (communication with GUI software) SU-CONNECT Sensor connection SU Vessel LIGHT...
  • Page 10 /// Vessel 0.5 L Single-wall glass vessel 0.5 L 10 L Double-wall glass vessel 0.5 L 10 L /// Lid Port sizes Ports 0.5 L 10 L Temp Pg13.5 Level Baffle 11 mm Pg13.5 Exhaust cooler Harvest Sparger 11 mm Feed 11 mm (x2) 4in1/Feed...
  • Page 11 /// Lid with plugs and fittings /// Harvest pipe 10 mm 10 mm 10 mm 10 mm 10 mm 15 mm 10 mm 18 mm 10 mm 10 mm 10 mm 3.2 mm /// Drive shaft /// Ring or micro sparger 4.8 mm Turn 10 mm...
  • Page 12 /// Baffle /// Level sensor /// Temperature sensor Turn 10 mm /// Condenser Danger! /// DO, pH, CO , conductivity or turbidity probe › During autoclaving, there must be no water in the condenser. Fill the condenser with water only after autoclaving! For autoclaving remove the filling screw of the condenser. Caution! Notice! ›...
  • Page 13 /// Spin filter (accessories) /// Vessel and stand Turn 10 mm Hold 10 mm HA.fi.x (accessories) /// Lid and vessel Notice! › Check the presence of the lid sealing. Never work without the lid seal. Risk of glass breakage.
  • Page 14 › Blue LED ON: Good alignment between sensor boxes, and no foam present. Notice! › Blue LED OFF: Poor alignment between sensor boxes, or with obstructions in LED field of view. › The Peltier cooling may only be used in conjunction with the IKA HABITAT. Foam sensor setup Left...
  • Page 15 /// Heat blanket /// Pump tubing › Flow rate Warning! › Ensure that the electrical equipment is in good condition before use. Flow rate (ccm / min) › For a long service life: Do not cut or fold! Tube ID Pump head adjustment min.
  • Page 16 /// Hose connecting /// Connecting gas outlet to sparger › Keep the hose connection as short as possible. Connecting gas outlet to sparger: with barb connector Flask stand Plug in barb connector (HA.sfr.250, accessories) 6 mm x 5 mm Silicone tube 4.8 mm x 9.5 mm x 3 m Filter Y hose connector...
  • Page 17 Turb* Turbidity Sensor Heat Blanket Filter Heater DO Sensor HA.ls.2 HA.ls.10 4 gases system / IKA HABITAT Cell growth & Ferment * Accessories ** Cell growth 0...2000 ccm Ferment 0...20000 ccm 1 bar Level Air 3 bar max 1 bar Temp 0...2000 ccm**...

  • Page 18: Operation

    1.2 Installing the HABITAT app 2.3.1 Operating mode › Go to the App Store and search for "IKA HABITAT" or scan the QR code below to install the › The user can select and set the operating app.
  • Page 19 Same network Ethernet Ethernet › Open the IKA HABITAT software and select "Scan for new Device". › Open the IKA HABITAT software and select "Scan for new Device". - The software shows the available devices detected from the Ethernet port.
  • Page 20 › Open the IKA HABITAT software and select "Scan for new Device". › Enter the password “000000” (default password for logging in). - The software shows the available devices detected from the Wi-Fi SSID: › Open the IKA HABITAT App and select "Connect to Wi-Fi… ".
  • Page 21 › Click to return to the IKA HABITAT App. - Enable the "Auto-join" only for pairing with the controller unit to establish a stable connection between iPad and control unit. › Select "Scan for new Device". - The software shows the available devices detected from Wi-Fi.
  • Page 22 2.3 iPad cable connection › Set Ethernet connection for iPad (one-time setting): 1 Select "<…> Ethernet" › Backup solution for cable connection from iPad to the control unit 2 Select "USB 10/100 LAN" 2.31 Components 1 Lightning Ethernet Adapter (with the charging port) 2 LAN cable 2.32 Connection 3 Select “Configure IP”...
  • Page 23 4 Select “Manual” 5 Set the IP Address / Subnet Mask / Router: IP Address: 192.168.10.10 Subnet Mask: 255.255.255.0 Router: 192.168.10.251 6 “Save” the setting › HABITAT software connect 1 Open HABITAT software 2 Select "…" at "Device Connection" 3 Select "Enter manually" 4 Select communication type as "Ethernet"...
  • Page 24 › Open HABITAT App, and select "Connect to Wi-Fi…". › Click to return to the IKA HABITAT App. › Connect to the HABITAT control unit via Wi-Fi SSID: IKA_HABITAT_xxxxxxxx - Enter the password “ikahabitat” (only necessary for the first connection) to start the connection.
  • Page 25 /// Menu structure IMPORTANT NOTE! › Disable "Auto-join" for Wi-Fi connection to other devices to establish a stable connection between Tablet and Control Unit. Available Scan for new Device Device Enter manually Communication Wi-Fi Host name Type Port Ethernet Host name Port Serial COM port...
  • Page 26 Process view Batch Batch ID On delay information On time Name Sensitivity Recipe Level limit Mode Status Auto Elapsed Time On delay Note On time Start Button Start Cultivation Entering batch name Sensitivity Entering note / comment Level control Harvest Choose recipe mode Feed...
  • Page 27 Setup view General Batch mode Batch Select output Overlay path for Air Sparger Fed-batch Overlay & Sparger Perfusion Select output Overlay Select logging 5 sec path for O2 Sparger interval 10 sec Overlay & Sparger 30 sec Select output Overlay 1 min path for N2 Sparger...
  • Page 28 Trend view Trend settings Enable Alarm Plot Parameter Stirrer Timestamp Temp Error code Description Value Acid Base Anti-foam Mantenance Sensor Temp Last Calibration Level Calibration Serial no. Zero Slope Condition Light Action CO2 probe Last Calibration Serial no. Turbid Zero Plot Color Slope Condition...
  • Page 29 Device type Not set HABITAT Cell HABITAT Ferment HABITAT Cell Photo HABITAT Ferment Photo HABITAT Cell CCT HABITAT Ferment CCT HABITAT Cell Photo CCT HABITAT Ferment Photo CCT Logging level Info Debug Log file to keep (days) 21-CFR Mode enabled Info...
  • Page 30 1.2 Screen Layout Mobile › Click on the plus sign "+" to open menu for re-scanning of new devices or entering the information to connect the device manually if it’s not discovered by the software. › If the device is turned on, but not discovered by the software, you can enter the information manually to connect the device.
  • Page 31 2.2 Connection screen 2.3 Application Header Menu › The software connects to the device and loads all necessary data. 2.3.1 Header TAB Button › During data synchronization, the connection may be interrupted due to the poor connection › For screen navigation 5 Historical data view quality or the invalid data.
  • Page 32 2.5 Process view 2.5.2 Login › Start screen with schematic illustration of the reactor vessel: › Before the user is able to change any parameters value or configuration, the user is required - Display of components of the current configuration to log in to the device first.
  • Page 33 2.5.3 Stirrer Control 2.5.4 Gas Control (Air, O2, N2, CO2) › Mode: › Mode: - Off: Turn off the stirrer - Off: Turn off the gas module - Manual: Set the stirring speed manually (independent of other parameters). - Manual: Set the gas flow rate manually (independent of other parameters). - Cascade –...
  • Page 34 2.5.5 Gas Mixer (Air, O2) › Gas Mixer: Set Air and O2 control mode to Manual mode. › If the Gas Mixer is enabled the set point input from Air and O2 control will be disabled. › Set flow: Enter flow rate in vvm (Volume Vessel per Minute), up to 2 vvm. ›...
  • Page 35 2.5.7 Exhaust Control 2.5.9 Level limit Control › Mode: › Mode: - Off: Turn off - Off: The sensor will detect the level but will not take any further action. - Auto: The controller will automatically control the filter heating temperature according to - Auto: If the level limit is detected, the controller will turn on the level pump to feed / the setpoint value.
  • Page 36 - Auto: Enter pO2 value (% saturate) with the Cascade control setting in the setup. › Actuator: Select Heat blanket / Thermostat (IKA HRC 2) to control the temperature of the - Profile: Preset of up to 10 values to be run within a specified time period.
  • Page 37 2.6 Setup (Recipe) Menu 2.6.2 Reactor 1 Reactor vessel type and size › Create, save and restore the setup: - Reuse the previous configurations for the experiment. (To effect min/max limits of parameter such as Gas flow rate and reactor vessel visualization) - Generate the experiment report after the experiment is finished.
  • Page 38 2.6.3 Pumps 2.6.4 Gases 1 Enable / Disable up to 4 gas modules for the experiment. › The control unit consists of 4 built-in pumps: Pump A (top) to Pump D (bottom). › It is also possible to add an external pump called Ext A. 2 Enable / Disable the Gas Mixer (Air, O2).
  • Page 39 2.6.5 Controllers 2.6.5.1.1 DO cascade control (only) with the stirrer 2.6.5.1 pO2 controller › To have the DO control by the stirrer speed adjustment, and then the constant flowrate for 1 Enable / Disable parameters to control the pO2 value the gas input.
  • Page 40 › Operating the Stirrer in “Cascade - DO control” mode 2.6.5.1.2 DO cascade control by the stirrer and the gas flowrate adjustment 1 Open the "Stirrer" module › Set the “DO cascade table” Option 1: To have DO control by stirrer speed and Gas flowrate adjustment in the same time. 2 Select the mode "Cascade –...
  • Page 41 › Operating the PO2 control in “Auto” mode Option 2: To have DO control by stirrer speed as the first step, and then the gas flowrate 1 Open the "pO2" module adjustment in the second step. 1 Select the tab “Setup recipe” 2 Select the mode "Auto"...
  • Page 42 › Operating the Gas in “Cascade - DO control” mode 2.6.5.2 pH controller 1 Open the "Air“ module 1 Enable / Disable the parameters to control the pH value 2 Select the mode ”Cascade – DO control“ 2 Select the output path 3 Click ”Apply“...
  • Page 43 › pH control with ACID and BASE pump › Setting mode “Auto” for pH module 1 Open the "pH" module › Setting “Auto – pH control” for ACID and BASE pump 1 Open the "Acid" pump module 2 Select the mode "Auto" 2 Select the mode "Auto –...
  • Page 44 2.6.6 Cell & Medium information 2.6.7 Accessories & Option 1 Input of cell / product information 1 Enable / Disable Light panel 2 Input of culture medium information 2 Enable / Disable CO2 sensor 3 Input of supplement information 3 Enable / Disable Conductivity sensor 4 Enable / Disable Turbidity sensor 5 Enable / Disable External signal module 6 Enable / Disable External signal module...
  • Page 45 › Table view of all parameters: › Graphical display of current data. - Actual value › Data is updated when the software is connected to the IKA HABITAT or when the device - Set value time is changed. - Unit ›...
  • Page 46 2.9 Historical data 1 Enable / Disable Trend display 1 Table view of all the experiments 2 Select the parameters to be displayed 2 Graph display of historical data series 3 Select the color 3 Button to export graphical data (PNG format) 4 Enable / Disable the function of auto scaling 4 Graph legend, click to show / hide 5 Set the minimum Y-Axis scaling...
  • Page 47 2.10 Alarm view 2.11 Maintenance Menu › The screen shows the alarm messages for the running experiment. If no experiment is run- 2.11.1 Sensor Calibration ning, the alarm messages will not be recorded. 1 Date / time of last calibration ›...
  • Page 48 2.11.2 Pump Calibration 2.11.3 Controller 1 Date / time of last calibration › This menu is intended for the experienced users with knowledge of PID controller systems to 2 Tubing inner diameter. enter their own PID controller parameters. 3 Set value of the last pump calibration 1 kp constant for the PID controller.
  • Page 49 2.12 Settings Menu 2.12.2 Alarm setting 1 Enable / Disable alarm limits 2.12.1 General 1 Change the device name 2 Lower limit: If the actual value is lower than the lower limit, an alarm will be triggered. 2 Select the user language 3 Higher limit: If the actual value is higher than the higher limit, an alarm will be triggered.
  • Page 50 5 Shop: Link to HABITAT product page on IKA homepage › Access to this menu is reserved for Admin Level users! › However, some menus require IKA service permissions with a service password to access and change the parameters. 1 Restore factory default configuration.
  • Page 51 /// Autoclaving › Before autoclaving, please remove the motor and keep the connectors of double-walled glass vessel open! › During autoclaving, there must be no water in the condenser. Fill the condenser with water Danger! only after autoclaving! For autoclaving remove the filling screw of the condenser. ›...
  • Page 52 /// Inoculation /// Harvesting › Blow torch flame over the coupling to sterilize again before opening it for inoculation. › There are different methods for harvesting or emptying the reactor vessels: › There are different options for the inoculation: - Direct pouring into an empty vessel or transferring to another vessel under the laminar flow - Use a syringe and the septum port.
  • Page 53 › USB Cable A – B: This cable is required to connect USB port to a PC. https://www.ika.com/ika/lws/download/usb-driver.zip Install the driver by running the setup file. Connect the IKA device through the USB data cable to the PC. The data communication runs through a virtual COM port.
  • Page 54 Ethernet Router & hub Ethernet Internet Ethernet, WiFi Ethernet › Ethernet + WiFi Internet WiFi, 3G / 4G Ethernet + WiFi › IKA Cloud GUI (Windows) Ethernet WiFi Router IKA Cloud IKA Cloud Ethernet (iOS App) Router & hub Internet...
  • Page 55 /// Cleaning › For cleaning disconnect the mains plug! /// Maintenance Checklist › Use only cleaning agents which have been approved by IKA to clean IKA devices: Water containing surfactant / isopropyl alcohol. › Wear protective gloves during cleaning the devices.
  • Page 56 Error codes 01172915 - Level Sensor disconnected Causes › No signal from sensor Effect › Level control is not active /// Table "Error codes" Solutions › Check the cable connection › Check the sensor › The fault is shown by an error code on the display as following if the error occurs. ›...
  • Page 57 01573610 - Pump B Speed blocked 1.2 Temp 1.2.1 Color status: Causes › Pump B does not run › GREEN: Temperature control is working normally. Effect › Pump B stops › ORANGE: Warning, actual value out of the alarm limits Solutions ›...
  • Page 58 Technical data 2. Interrupting Status 2.1 Emergency mode display › All 6 LEDs keep flashing in “RED” Environment HABITAT Permissible ambient temperature + 5 ... + 40 °C safe STOP Permissible relative humidity 80% (up to 31 °C), decreasing linearly to max. 50 (@40 °C) Permitted on-time 100 % Stirrer Temp...
  • Page 59 Pump Module Vessel information Glass Glass Glass Glass Glass Quantity 4 PC Unit vessel vessel vessel vessel vessel Controller Variable speed (CW / CCW) 0.5 L 10 L ID 0.5 mm: 1 ... 4 ccm/min Total volume 3.00 12.9 ID 0.8 mm: 3 ... 12 ccm/min Max.
  • Page 60 Overall dimension (mm) Space requirement for autoclaving (mm) Vessel size Width Depth Height Vessel size Width Depth Height Glass vessel 0.5 L Glass vessel 0.5 L Glass vessel 1 L Glass vessel 1 L Glass vessel 2 L Glass vessel 2 L Glass vessel 5 L Glass vessel 5 L Glass vessel 10 L...
  • Page 61 Multiuse vessel Peltier Cooling Material Type of cooling peltier or water cooling Vessel Borosilicate glass 3.3 Temperature min. / Condenser with Peltier 15 °C Components contact to medium Stainless steel 316 Operating mode continuous operation Components not contact to medium Stainless steel 304 Auto restart after power failure Gasket...
  • Page 62 Warranty PC requirements Minimum Recommended › In accordance with IKA warranty conditions, the warranty period is 24 months. For claims Operating system Windows 10, 32/64 bits Windows 10, 64 bits under the warranty please contact your local dealer. You may also send the device direct to...
  • Page 63 Phone: +84 28 38202142 Phone: +66 2059 4690 Phone: +90 216 394 43 43 eMail: sales.lab-vietnam@ika.com eMail: sales.lab-thailand@ika.com eMail: sales.turkey@ika.com Discover and order the fascinating products of IKA online: www.ika.com IKAworldwide IKAworldwide /// #lookattheblue @IKAworldwide Technical specifications may be changed without prior notice.

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