Flushing The Ibp Transducer And Cannula; Making Blood Pressure Measurements - SA Instruments 1030 Operation Manual

Invasive Blood Pressure
metal stub can be inserted into one end of a length of PE50 tubing to fabricate the
cannula.
When using the IBP function in the MR environment, make certain the tubing is no
longer than necessary to extend from the animal in the bore to the IBP transducer
location at the entrance of the bore, typically 1 to 1.5 m.

Flushing the IBP transducer and cannula

Before insertion of the cannula in the animal, the IBP transducer and cannula must be
flushed and filled with heparinized saline. The tube and cannula must be completely
filled with fluid (i.e. no air bubbles). Failure to eliminate entrapped air can result in
inaccurate blood pressure readings due to the compressibility of air.
Flush the transducer and cannula by connecting a syringe or elevated IV bag to the port
at valve #2. Opening valve #2 allows fluid to pass through and out the end of the
cannula. Air bubbles can be eliminated by raising or lowering and tapping on the
tubing. Note the three-way stopcock should be positioned to block the port with no
connection (open to air).

Making blood pressure measurements

The following steps to make IBP measurements should be integrated with animal
handling and aseptic protocols established for your facility:
Connect the transducer with cannula to the IBP port on the IBP Module. If necessary
use an adapter cable to make the connection. Select the IBP3 option in the monitor's
SETUP MENU. A flat waveform should be present on the IBP portion of the monitor's
display. The IBP waveform amplitude should change in response to pressure variations
on the transducer.
Flush the transducer and cannula with heparinized saline solution. Remove all air
bubbles.
When using the transducer in the MR environment, it should be constrained from
moving in the magnet's fringe magnetic field. After positioning the IBP Module near the
entrance to the magnet bore, tape the transducer to the canopy or bed near the
entrance to the bore. Position the transducer at the level of the animal to maximize
accuracy of Blood pressure measurements. Transducer offset changes with the
magnetic field, so after securing the transducer it should be zeroed. Check to make
certain air bubbles were not introduced.
Establish an invasive line in the animal by inserting the cannula according to the
surgical protocol for your facility. Add additional heparinized saline if necessary to
position the blood saline interface between the animal and the transducer. Repeat as
necessary to keep blood from entering the transducer. A blood pressure waveform
should be present on the monitor display.
Revision C, August 2013
10-5