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SA Instruments 1030 Operation Manual

Mr-compatible small animal monitoring and gating system
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Model 1030
MR-compatible
Small Animal Monitoring
and Gating System
Operation Manual

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Summary of Contents for SA Instruments 1030

  • Page 1 Model 1030 MR-compatible Small Animal Monitoring and Gating System Operation Manual...
  • Page 2 Mouse aortic root study 9.4T vertical field ECG and respiratory gated 90 second image, without contrast Mount Sinai School of Medicine, New York, NY Departments of Radiology and Cardiology Zahi A. Fayad, PhD Juan Gilberto Aquinaldo, MD Vitalii V. Itskovich, PhD Mark Lieb, MD Copyright ...

  • Page 3: Table Of Contents

    Table of Contents Introduction ....................... vii Chapter 1: System Overview ...................... 1-1 Monitoring and gating ....................1-1 System components ....................1-1 Chapter 2: System Setup ......................2-1 Installation of software ....................2-1 Setting up the hardware ....................2-1 Communication port assignment ................. 2-3 Setting the communication port in PC-sam ..............
  • Page 4 Table of Contents Positioning the thermister temperature probe .............. 4-7 Charging the ERT Battery Pack ................... 4-7 ERT Battery Pack Protection Circuit ................4-8 Setting the 50/60 Hz notch filter ................... 4-8 Chapter 5: Simulator ........................5-1 Overview ........................5-1 Operation ........................
  • Page 5 Table of Contents Chapter 9: Additional Features....................9-1 SYSTEM INFORMATION window ................9-1 SET COLORS window ....................9-2 ALARMS window ......................9-3 Cardiac and respiratory sweep gates ................9-4 Freeze/thaw key ......................9-5 Event key ........................9-5 Chapter 10: Invasive Blood Pressure ..................10-1 Overview ........................
  • Page 6 Table of Contents Chapter 13: Fiber Optic Pressure ....................13-1 Overview ........................13-1 Sensor extensions and connections ................13-1 Fiber optic communication cable connections ............13-2 Handling and cleaning the fiber optic pressure sensor ..........13-2 Display configuration ....................13-4 Calibrating fiber optic pressure sensor ...............
  • Page 7 Table of Contents Chapter 16: Signal Breakout Module .................... 16-1 Overview ........................16-1 Input/output connections ....................16-1 Setup and use ......................16-2 Using multiple Signal Breakout Modules ..............16-3 Signal Breakout Module data formats ................. 16-4 Capturing ASCII slow data from the serial output ............16-4 Capturing ASCII waveform data from the serial output ..........
  • Page 8 Table of Contents Appendix A: Product Specifications ..................... A-1 Appendix B Supplies and Accessories ..................B-1 Appendix C: Troubleshooting ......................C-1 ERT communication error ................... C-4 Check communication between the PC and ERT Control/Gating Module ....C-4 No heat from the heater ....................C-5 Low heat from the heater ....................

  • Page 9: Introduction

    Small Animal Monitoring and Gating System. Nearly every major medical school in the world has at least one of SA Instruments monitoring and gating systems and many have several. We are grateful to all those medical researchers who have used SAI equipment and offered constructive comments allowing us to improve the performance and capabilities of our systems.
  • Page 10 Introduction Revision C, August 2013 viii...

  • Page 11: Chapter 1 System Overview

    Chapter 1 System Overview...

  • Page 13: Monitoring And Gating

    System Overview Monitoring and gating The Model 1030 monitoring and gating system was designed specifically to meet the physiological monitoring and gating needs for anesthetized mice, rats and larger animals in the high field MR environment. Both anesthesia and the local environment can adversely alter animal physiology.
  • Page 14 Heater Module to regulate the animal’s temperature. The following options are available for use with the Model 1030: invasive blood pressure (IBP) measuring systolic, diastolic and mean arterial pressure, pulse oximetry...
  • Page 15 System Overview Revision C, August 2013...
  • Page 16 System Overview Revision C, August 2013...

  • Page 17: Chapter 2 System Setup

    Chapter 2 System Setup...

  • Page 19: Installation Of Software

    “Power Management Off”. Setting up the hardware The Model 1030 has several optional functions. This manual assumes all functions are present with the system. Ignore those sections of the manual for options not present with your system. Refer to Appendix B for a list of major system components and accessories.
  • Page 20 System Setup 2. Connect the ERT Control/Gating Module (720200) to the PC using the serial interface cable (CGIC-201). 3. Power the ERT Control/Gating Module with the external 12 VDC power supply (PS-2-12-CG). 4. Connect the ERT Control/Gating Module to the ERT Module (110100) using the simplex fiber optic cable.

  • Page 21: Communication Port Assignment

    System Setup Communication port assignment Windows will automatically assign a communication port number for the serial or USB port which is being used for communication to and from the ERT Control/Gating Module. The assignment is made when the PC is turned on. The most common port assignments are “com1”...

  • Page 22: Testing The Heater System

    System Setup Test the Respiration (P-resp) function of the ERT Module by connecting a Respiration Pillow Sensor (RS-301) to the pneumatic port on the ERT Module. Lightly touching the pillow sensor should produce waveform deflections on the PC’s Resp display.. Testing the heater system Activation of the Fan Module power switch should produce air flow to the Heater Module.

  • Page 23: Step By Step Instructions For Monitoring And Gating

    System Setup Step by step Instructions for monitoring and gating Click the PC-sam icon on the desktop > select “Start Monitor with Selected Setup” to view the main monitor display screen. The “selected setup” should be “factory defaults” which includes ECG, respiration from the pillow sensor, temperature from the thermister probe and ECG gating.

  • Page 24: Recommendations For Daily Operation

    System Setup in milliseconds for the cardiac or respiratory cycle. Refer to Chapter 3 for additional information regarding the main monitor display Recommendations for daily operation Connect the ERT Module Battery Pack to the charger at the end of the day. The battery pack can be left on the charger when not in use.

  • Page 25: Pc And Ert Control/Gating Module

    Chapter 3 PC and ERT Control/Gating Module...

  • Page 27: Overview

    Chapter 3 PC and ERT Control/Gating Module Overview The ERT Control/Gating Module, located near the operator’s console, is connected to a PC to provide operator control as well as display and storage of waveforms, computed gates, measured values and trends. The optical signal received at the ERT Control/Gating Module from the ERT Module is demodulated and separated into ECG, respiration from the pneumatic pillow sensor and temperature components before being processed to detect the R-wave (primary) peak...
  • Page 28 PC and ERT Control/Gating Module Gates and waveforms sweep from left to right. The number of red dots (R-waves) determines the measured heart rate and the R to R period. Likewise the number of red bars (expirations) generates the measured respiration rate and period. Revision C, August 2013...
  • Page 29 PC and ERT Control/Gating Module Temperature measurements are displayed in a 5 minute trend with the most recent measurement on the right of the display. Each new temperature measurement is displayed in digits as it is entered in the trend portion of the display. Located along the bottom of the monitor display are several keys which perform special functions, display data or allow user entry of operating parameters.

  • Page 30: Labeling Convention For Respiration Channels

    PC and ERT Control/Gating Module boxes to increase or left click to decrease the sweep time. Note the cardiac and respiratory sweep times are independent and can be set to different values. Switch between ºC and ºF by clicking on the digits in the temperature display Clicking on the speaker icon associated with the cardiac sweep activates an audible beep for each ECG R-wave.

  • Page 31: Ert Control/Gating Module Input/Output Connections

    PC and ERT Control/Gating Module ERT Control/Gating Module input/output connections Electrical connections: Gate output gate to trigger scanner Serial connection to PC RESP GATE output respiration gate to trigger scanner Power 12 VDC input power connection Aux gate input, a user TTL input gate channel Fiber optic connections: Input from the ERT Module AUX GATE IN...
  • Page 32 PC and ERT Control/Gating Module 5. Change the serial communication port 6. Select a default stored setup file at startup 7. Select an alternate stored setup for the current session. Revision C, August 2013...

  • Page 33: Snapshot Key

    PC and ERT Control/Gating Module The portion of the setup window for waveform, gate and trend selection includes sections for cardiac, respiration and temperature. The numbers to the right of the trace selections are the number of vertical pixels necessary for that portion of the display. The total number of pixels used can not exceed the number available for the PC’s display.

  • Page 34: Gating Setup Window

    PC and ERT Control/Gating Module GATING SETUP window Click the Gating key to open the GATING SETUP window. This window provides a means to include one or more signals in the gating algorithm. In the window above, both ECG and respiration gates must be satisfied for generation of an output gate (white dot).

  • Page 35: R-Detect Setup Window

    PC and ERT Control/Gating Module R-DETECT SETUP window Click the ECG button in the GATING SETUP window or right click in the ECG waveform display to view the R-DETECT SETUP window. The high pass filter removes low frequency components of the ECG waveform. It can be useful with large animals and low heart rates, but is of little benefit for mice.
  • Page 36 PC and ERT Control/Gating Module The R-detect algorithm is based on 2 or 3 points and their positive and/or negative slew rates or slopes (amplitude/time). The waveform shown exhibits R-wave gate generation for factory defaults: positive peak with positive slope of 200µV/6.7ms and a negative slope of 98µV/4.4ms.

  • Page 37: Charger Window

    PC and ERT Control/Gating Module enough not to get false gates, then slowly increase one of the amplitudes until gates begin to be missed. Final setting of 30 - 50% of max works well with auto detection threshold and 60 – 80% for manual detection threshold. The auto detection threshold is a proprietary additional detection criterion.
  • Page 38 PC and ERT Control/Gating Module Revision C, August 2013 3-12...

  • Page 39: Chapter 4 Ert Module

    Chapter 4 ERT Module...

  • Page 41: Overview

    Chapter 4 ERT Module Overview The animal’s electrocardiograph (ECG) is obtained from two or three leads connected to sub-dermal needle electrodes, gold disk surface electrodes or pads. A respiration signal may be superimposed on the measured ECG waveform by closely coupling one of the active ECG lead wires to the animal’s abdomen.

  • Page 42: Attaching Ecg Electrodes

    ERT Module Connecting the Battery Pack Cable The Battery Pack Cable attaches to the ERT module and Battery Pack using small Lemo connectors. To make the connection, align the red dots and push the connector straight into the receptacle. To disconnect the sensor grasp the connector with the thumb and index finger near the red dot and pull straight back.
  • Page 43 ERT Module tape. The wire bundle should then be taped to the animal holder to eliminate movement from gradient vibration and/or air flow. Movement of the ECG lead wires will create artifacts in the ECG waveform. Caution: ECG lead wires should be twisted and unnecessary loops should be avoided.
  • Page 44 ERT Module animal. When using a small whole body coil, it may be necessary to connect the leads to both front legs or both rear legs keeping the lead wires out of the imaging coil. E-resp™ without a cradle It is possible to obtain adequate coupling of an ECG lead wire to the animal’s abdomen without the use of a cradle.
  • Page 45 ERT Module With cradle Without cradle Revision C, August 2013...

  • Page 46: Attaching The Temperature Probe

    ERT Module Attachment of the respiration pneumatic pillow sensor Position the pneumatic pillow sensor near the animal’s abdomen and secure it with tape as shown in the photograph. Attach the sensor’s tube to the ERT Module at the Leur connection. It may be necessary to use one of the Respiration Extension Tubes to make the connection.

  • Page 47: Positioning The Thermister Temperature Probe

    ERT Module Temperature probes are fragile and should be handled with care. The tip of the probe can be broken or cracked if the tip is bent. Problems with the temperature probe can occur if it develops a crack. Since the thermister in the tip of the probe operates at 5 V and a break lets conductive fluid from the animal make contact with the thermister wire, a small offset voltage can occur.

  • Page 48: Ert Battery Pack Protection Circuit

    ERT Module To obtain maximum battery life and performance: • Recharge after each use • Store in a charged state • Store at room temperature or lower • Recharge stored batteries every 9 months ERT Battery Pack Protection Circuit ERT Battery Packs have an internal protection circuit. The circuit opens to protect the battery if the battery voltage drops below a preset threshold.

  • Page 49: Chapter 5 Simulator

    Chapter 5 Simulator...

  • Page 51: Overview

    Chapter 5 Simulator Overview The simulator generates an ECG voltage waveform with respiration and gradient pulse interference superimposed. Connection to the ERT Module is from the simulator rear panel connections with simulator ECG leads to black, red and white labeled studs. The simulator also provides a reference temperature impedance.

  • Page 52: Operation

    Simulator Operation The Simulator is powered by a 9V internal battery. The power switch on the front panel activates the ECG and Respiration waveform. Green LEDs flash with the heart QRS and with respiration. A yellow LED flashes when the internal battery is low. Separate controls are provided for ECG rate and amplitude and respiration rate and amplitude.

  • Page 53: Battery Replacement

    Simulator Battery replacement The simulator is powered by a 9 volt battery. Replace the battery when the battery low indication illuminates. To replace the battery, remove two screws from the simulator case and remove the simulator top. Replace the screws to reattach the cover after replacing the battery.

  • Page 54: Capnograph And Ventilator Option

    Simulator Capnograph and ventilator option Simulators can have, as an option, outputs to simulate a capnograph and a ventilator. In that case, in addition to ECG and temperature outputs the simulator will have the following connections and controls: SYNC OUT ventilator output for I/E ratio and respiration rate SERIAL OUT...

  • Page 55: Chapter 6 Air Heater System

    Chapter 6 Air Heater System...

  • Page 57: Overview

    Chapter 6 Air Heater System Overview The Air Heater System controls the temperature of small animals undergoing imaging procedures. Animal temperature is measured using the rectal probe. The software continuously processes temperature measurements and sends a control signal to the Heater Module.
  • Page 58 Air Heater System Revision C, August 2013...

  • Page 59: Heater Control

    Air Heater System Heater control Clicking the Heater button at the bottom of the main waveform display in PC-sam opens the main HEATER window. The window can be used to select which temperature probe is to be used to control the heater (thermister or fiber optic), to enter the set point control value (usually around 37 ºC) and to set the maximum heat output (0 –...

  • Page 60: Heating The Mouse

    Air Heater System On/off control with manual control of the heater duty factor should be selected when using the SAII Air Heater System. Here, heater duty factor, PWM max heat and Maximum Heat Output all refer to the same setting. When the mouse temperature is several degrees below the desired set point, the duty factor should be set near 100%.

  • Page 61: Chapter 7 Trends

    Chapter 7 Trends...

  • Page 63: Overview

    Chapter 7 Trends Overview During operation, the Windows based PC-SAM software continuously logs data to a trend file located in a project folder. The data sample rate is once per second and more than 70 items are included in the recorded data point. The items include measured values of physiological functions (e.g.
  • Page 64 Trends Several keys are located on the bottom of the display along with a message area. The following keys allow the data set to be retrieved, reconfigured and exported: Source Trend – opens a trend data file Get a Setup – opens a setup file for display of data Save a Setup –...

  • Page 65: Source Trend Key

    Trends Source Trend key Clicking this key opens a window that allows selection of a trend data set. The window opens when TrendMap is executed or when the Source Trend key is clicked. Get and Save Setup keys These keys allow files which define the setup to be retrieved and saved. It can be useful after defining the order of trends in the data set (i.e.

  • Page 66: Mark Stop And Mark Stop Keys

    Trends Clicking the “Mark as Last Record for Output” key defines the trends which will be included in the exported data set. The trends to be exported (number 1 through the last record) are indicated in green while all others are indicated in red. Mark Start and Mark Stop keys Mark Start and Mark Stop keys define the time portion of the exported data set.

  • Page 67: Selecting Events Recorded In Trend Data

    Trends The OUTPUT FILE SETUP window allows the following items to be defined for the exported data file: Header information Time column format Seconds per output line Trends per output line Column and line delimiters Number for bad data The time exported can be as seconds from the start or actual time. Usually one second is output per line, but very large data sets may necessitate multiple seconds per output line.

  • Page 68: Trend Data Item List

    Trends Trend data item list The following table lists the items recorded in trend data. Item # Label Unit explanation ECG rate heart rate from ECG leads Resp Rate resp/m respiration rate from the pneumatic pillow input Temp 1 ºC or F temperature from channel 1 SpO2 % Sat...
  • Page 69 Trends Item # Label Unit explanation USER Period period from USER Resp channel Pambient mmHg ambient barometric pressure Temp; 2 ºC or F temperature from channel 2 Event text users text message input Vbatt volts battery voltage of ERT Battery Pack Voffset volts offset voltage of ECG leads measurement...
  • Page 70 Trends Revision C, August 2013...

  • Page 71: Chapter 8 Snapview

    Chapter 8 SnapView...

  • Page 73: Overview

    Chapter 8 SnapView Overview SnapShot data files record all measured data for a short time interval. The SnapShot key is located in the lower left portion of PC-sam’s main display. Clicking the key causes storage of the last 36 seconds of all measured data. SnapShot data files are viewed with SnapView.

  • Page 74: Setup Window

    SnapView SETUP window The SETUP window is displayed when SnapView is executed or when the Set-Up key is clicked. First select and open a SnapView data file, then make any desired changes to the display configuration before displaying SnapView data. Two keys for data display are available: Run SnapShot Viewer and Quick View SnapShot.

  • Page 75: Rerun Key

    SnapView ReRun key Click the ReRun key on the SnapView display to play back the 36 second data set. Print key The print key will print a frozen display. The desired printer should be selected as the default printer and the paper orientation should be set to landscape before running SnapView.
  • Page 76 SnapView Revision C, August 2013...

  • Page 77: Additional Features

    Chapter 9 Additional Features...

  • Page 79: System Information Window

    Chapter 9 Additional Features SYSTEM INFORMATION window The SYSTEM INFORMATION window lists software revisions, current status for the ERT Module and other useful information. The window will automatically close after several seconds. Click the “Hold Open” button to keep the window visible. ERT Module status includes the 50Hz/60Hz notch filter setting, battery voltage, ECG and temperature lead status and offset voltage.

  • Page 80: Set Colors Window

    Additional Features SET COLORS window The SET COLORS window allows selection of colors for regions of the display and for waveforms and digits. The set button sets the current color for the region or waveform selected. Colors can be selected from Quick Pick or made from combinations of red, green and blue in the upper left of the window.

  • Page 81: Alarms Window

    Additional Features ALARMS window The ALARMS window allows high and low alarm limits to be set for heart rate, oxygen saturation, respiration rate and/or temperature. A check mark next to an alarm limit followed by clicking the Apply key will activate the alarm. When the measured value meets or exceeds the limit, an audible alarm sounds.

  • Page 82: Cardiac And Respiratory Sweep Gates

    Additional Features Cardiac and respiratory sweep gates Double clicking on the gray panel area of the cardio sweep display will open a window to allow other gates to be added to the selection list. A indicator can be added to the ECG waveform to display timing of the selected cardio rate gate or the output gate by clicking on the colored box to the left of the cardio rate gate selection.

  • Page 83: Freeze/Thaw Key

    Additional Features FREEZE THAW key The key freezes the waveform display. Pressing the key a second time restarts the waveform display. EVENT key Pressing (left clicking) the event key causes an event to be logged in the trend file at the time the event key is pressed.
  • Page 84 Additional Features Revision C, August 2013...

  • Page 85: Invasive Blood Pressure

    Chapter 10 Invasive Blood Pressure...

  • Page 87: Overview

    The order of the modules in the data loop is not important. But, in all cases the color of the connector must match the color of the port IBP transducers The Model 1030 is configured to operate using disposable invasive blood pressure transducers which meet the specification of 5µV/V/mmHg. The following IBP transducers meet this specification and can be used with the Model 1030.
  • Page 88 Invasive Blood Pressure Transducer name Manufacturer contact information Abbott Critical Care Systems Hospira, Inc. Transpac IV Lake Forest, IL 60045 (877) 946-7747, www.hospira.com Baxter Edwards TruWave Baxter/Edwards Lifesciences, LLC One Edwards Way Irvine, CA 92614 (949) 250-2500, www.edwards.com Utah Medical Deltran IV Utah Medical Products, Inc.

  • Page 89: Animal Cannula

    Invasive Blood Pressure three-way stopcock valve #1 connect to IV set or syringe valve #2 connect to remove boot and animal cannula connect to IBP connection on IBP Module Abbott Transpac IV IBP transducer The Abbott Transpac IV transducer can be connected directly to the IBP Module at the IBP connection.
  • Page 90 Invasive Blood Pressure IBP transducers have an electrical connection (for the IBP Module), three fluid connections (for the animal, syringe and/or IV set) and two valves. A very small piezoelectric pressure sensor is located in the fluid pathway between the two valves. Pressure changes caused by the animal’s blood in the tubing are converted to electrical signals by the transducer for display on the monitor.

  • Page 91: Flushing The Ibp Transducer And Cannula

    Invasive Blood Pressure metal stub can be inserted into one end of a length of PE50 tubing to fabricate the cannula. When using the IBP function in the MR environment, make certain the tubing is no longer than necessary to extend from the animal in the bore to the IBP transducer location at the entrance of the bore, typically 1 to 1.5 m.

  • Page 92: Zeroing The Invasive Blood Pressure Transducer

    Invasive Blood Pressure Most IBP transducers provide a specified level of electrical isolation for the animal. However, if additional isolation is desired, the IBP Module can be operated on internal battery rather than mains power. Zeroing the invasive pressure transducer The transducer must be zeroed to ensure that accurate absolute pressure values are displayed.

  • Page 93: Spare Channels For Auxiliary Data

    Invasive Blood Pressure Spare channels for auxiliary data Auxiliary analog input capability allows the user to acquire, record, display and gate from user generated analog waveforms. The capability exists for two analog channels. One channel sampled at the rate for respiration and a second channel sampled at the rate for blood pressure.
  • Page 94 Invasive Blood Pressure Revision C, August 2013 10-8...

  • Page 95: Chapter 11: Pulse Oximetry Module

    Chapter 11 Pulse Oximetry...

  • Page 97: Overview

    Chapter 11 Pulse Oximetry Overview Pulse Oximetry allows noninvasive monitoring of arterial blood oxygen saturation. Fiber optic oximetry sensors are used to transmit pulses of red and infrared light through the animal’s peripheral vascular region. Oxygen saturation is determined by measuring the differential absorption of the red and infrared light.

  • Page 98: Display Configuration

    Pulse Oximetry optional module and the other of the duplex fibers connecting to the last optional module. The modules are then connected to each other using short simplex fiber optic cables. The fiber optic oximeter sensor attaches to the module using a Lemo connector. To make the connection, align the red dots and push the connector straight into the receptacle.
  • Page 99 Pulse Oximetry To attach a fiber to the clip, use the index finger to open the clip and position the fiber as shown. Push the fiber into the large grove along the length of the clip making sure the collar is in close proximity to the end of the clip.

  • Page 100: Attachment Of Fiber Optic Oximetry Sensors

    Pulse Oximetry Attachment of fiber optic oximetry sensors The clip on sensors are typically attached to the rat foot or the shaved mouse thigh. For rabbits, the ear is often the location of choice A black mouse is shown above prepared for MR with ECG, pulse ox, respiration and temperature sensors.

  • Page 101: Pc-Sam's Main Display

    Pulse Oximetry The mouse tail/ankle fiber optic sensor is shown above attached to the ankle of a black mouse. The sensor head holding the two fibers simply has a hole where the tail or ankle can be inserted. Note that the form does not apply pressure to the attachment site, so oximetry signals tend to be robust.

  • Page 102: Plethysmogram Gate Threshold

    Pulse Oximetry Note the following: • There is a delay between the ECG and plet gates, because blood flows to a peripheral site after the heart beats. • There is continuous presence of baseline interference in the ECG waveform, because the MR scanner is running continuously. i.e. not being triggered. •...
  • Page 103 Pulse Oximetry The objective is to set the threshold so a gate is present above each peak in the pleth waveform with no extra gates. For mice, the signal strength will be 4 or more and the best selection for the threshold is 11 to 20.
  • Page 104 Pulse Oximetry Revision C, August 2013 11-8...

  • Page 105: Fiber Optic Temperature

    Chapter 12 Fiber Optic Temperature...

  • Page 107: Overview

    Chapter 12 Fiber Optic Temperature Overview Fiber optic temperature probes provide an alternative method to thermister temperature probes for measuring temperature in the MR environment. Fiber optic probes are not affected by the strong RF and magnetic fields of the MR scanner. They can not contribute to RF heating and they do not produce an artifact in MR images.

  • Page 108: Display Configuration

    Fiber Optic Temperature fiber optic communication cables. It receives power from a dedicated 12 VDC external power supply or via a daisy chain cable from one of the other modules. When Fiber Optic Temperature is the only option in use, the module connects to the ERT Control/Gating Module using a duplex fiber optic communication cable.

  • Page 109: Pc-Sam's Main Display

    Heater control The SA Instruments warm air Heater System can be controlled by the Fiber Optic Temperature module. The HEATER window can be used to select which temperature probe will be used to control the heater and to set the set point. Refer to Chapter 6 for a detailed explanation of the Heater System.
  • Page 110 Fiber Optic Temperature Revision C, August 2013 12-4...

  • Page 111: Chapter 13 Fiber Optic Pressure

    Chapter 13 Fiber Optic Pressure...

  • Page 113: Overview

    Chapter 13 Fiber Optic Pressure Overview The Fiber Optic Pressure option for the Model 1030 provides the capability to continuously monitor physiological pressure using ultra-miniature, fiber optic pressure sensors. The ultra-miniature sensors are intended for use in small infusion needles, catheters and guide wires enabling minimally invasive physiology pressure measurements.

  • Page 114: Fiber Optic Communication Cable Connections

    Fiber Optic Pressure Fiber optic communication cable connections When the Fiber Optic Pressure Module is the only optional module, it connects to the ERT Control/Gating Module using a duplex fiber optic cable. The cable should be connected to the fiber optic ports labeled “option in” and “option out” and the options I/O switch should be set to “options in use”.
  • Page 115 Fiber Optic Pressure The smaller sensor (FOP-3-3) has a polyimide protective sheath covering the pressure transducer. Cleaning the transducer end of the pressure sensor The transducer is glued to the fiber, but it can be broken off if not handled carefully. The sensor can not be mechanically cleaned by for instance rubbing with a tissue soaked in alcohol.

  • Page 116: Display Configuration

    Fiber Optic Pressure 2. Insert the tip of the cleaning stick in the SC female connector and rotate the stick. 3. Allow the SC connector to dry before inserting the SC male connector. Display configuration To add fiber optic pressure to the monitor configuration, execute PC-sam and select the option to “open the MONITOR SETUP window”.

  • Page 117: Zeroing The Fiber Optic Pressure Sensor

    Fiber Optic Pressure The Fiber Optic Pressure Module has two indicator lights and two push button switches as shown in the photograph below. A green light is present when power is on. A yellow light labeled “pulse” can have three conditions as follows: 1.

  • Page 118: Inserting The Fiber Optic Pressure Sensor

    Fiber Optic Pressure previous calibration parameters written to the EEPROM memory are automatically used to make the pressure measurement. Inserting the fiber optic pressure sensor The fiber optic sensor can be inserted at any location where a 22 gauge catheter can be inserted.

  • Page 119: Procedure For Making Ibp Measurements From The Rat Tail Artery

    Fiber Optic Pressure Procedure for making IBP measurements from the rat tail artery Heat the rat tail using warm water. A second set of hands is recommended to compress the tail artery and prevent bleeding. Insert the 22 gauge Terumo Surflo-W cannula with injection port in the tail artery outside the MR under a bright light.
  • Page 120 Fiber Optic Pressure Revision C, August 2013...

  • Page 121: Capnograph And Ventilator

    Chapter 14 Capnograph and Ventilator...

  • Page 123: Overview

    Chapter 14 Capnograph and Ventilator Overview The microCapStar Capnograph provides accurate end-tidal or continuous measurement of expired CO in animals as small as mice. It features very low sample flow requirements, rapid response time and long term stability. The CO waveform, respiration rate, end-tidal CO and minimally inspired CO...

  • Page 124: Ventilator Setup In Mr

    Capnograph and Ventilator Ventilator setup in MR The Ventilator Control Unit is positioned outside the magnet room. It is connected to the Valve Assembly using 23’ (7 m) long pneumatic tubes. Revision C, August 2013 14-2...

  • Page 125: Ventilator Operation

    Capnograph and Ventilator The diagram above shows connections between the Control Unit and the Valve Assembly as well as connections to components of an anesthesia setup. The ventilator should be located near a waveguide and accessible to the operator. Long cables are provided to connect to the Capnograph/Ventilator Interface Module.

  • Page 126: Capnograph Operation

    Capnograph and Ventilator The diagram above shows connections between the microCapStar and the animal. The measuring and control module is located outside the magnet room near a waveguide. A long tubing set is provided to connect to the animal in the magnet bore. Note: it is important to perform the CO2 calibration with the tubing set which will be used with the animal.

  • Page 127: Pc-Sam Main Display

    Capnograph and Ventilator PC-sam main display The PC-sam main display is shown on the next page configured with ECG, capnograph, ventilator and temperature. The CO waveform from the capnograph is displayed along with the respiration rate, respiration period, CO gate, end-tidal CO and minimally inspired CO .
  • Page 128 Capnograph and Ventilator Ventilator measurements are displayed below the capnograph display. Readings include respiration rate, tidal volume (TV), inspired to expired ratio (I/E), inspiratory flow and minute volume (MV). Revision C, August 2013 14-6...

  • Page 129: Chapter 15 Advanced Features

    Chapter 15 Advanced Features...
  • Page 131 Chapter 15 Advanced Features Manual E-Resp thresholds Respiration waveform gates are generated automatically for E-Resp , P-Resp User Resp. However, in the case of the E-Resp waveform, the option also exists to manually set the thresholds for gate generation. The E-RESP SETUP window can be opened by clicking on the E-Resp button in the GATING SETUP window.
  • Page 132 Advanced Features Revision C, August 2013 15-2...

  • Page 133: Inversion Timing

    Advanced Features Inversion timing For certain imaging sequences, such as inversion recovery, the image data is acquired at a time later than the start of the pulse sequence. In those cases it may be desirable to generate a delayed gate to make certain the image is acquired at a specific position in the cardiac and/or respiratory cycle.

  • Page 134: User Ibp Setup Window

    Advanced Features The waveform and scale labels are set in real time. They appear on the monitor display as they are being entered in the USER RESP window. The analog to digital converter (ADC) has 1024 bits. The user can select the scaling and offset between the ADC scale and the displayed scale.

  • Page 135: Auto Event For Time Stamping

    Advanced Features The following items can be set from the USER IBP window: 1. Labels for both waveform and scale 2. Scaling, 3. Offset, 4. Scale adjust step size 5. Signal gain The waveform and scale labels are set real time. They appear on the monitor display as they are being entered in the USER IBP window.

  • Page 136: Advanced Gating Setup Window

    Advanced Features ADVANCED GATING SETUP window The advanced gating menu includes the following additional features: 1. End Delay for the gate generated by each signal except ECG 2. Primary, begin, hold and end conditions for each signal gate included in the construction of the output gate 3.
  • Page 137 Advanced Features Note that the Apply and Cancel keys control the values entered for the auto track parameters. Pressing the Cancel key will display the previous value while pressing Apply sends the value to the ERT Control/Gating Module. The background color in the value box indicates the status.
  • Page 138 Advanced Features Revision C, August 2013 15-8...

  • Page 139: Signal Breakout Module

    Chapter 16 Signal Breakout Module...

  • Page 141: Overview

    Chapter 16 Signal Breakout Module Overview Physiological measurements and gates from the MR-compatible Model 1030 Monitoring and Gating System can be acquired by another data acquisition system or a PC using the Signal Breakout Module. In order to maximize data transmission rate, SA Instrument’s monitoring and gating systems send and receive densely packed data on the serial cable connecting between the ERT Control/Gating Module and the PC.

  • Page 142: Setup And Use

    Signal Breakout Module Physiological measurement output panel DAC1 Analog output 0 – 5 V channel 1 BIT1 Gate output 0 – 5 V channel 1 SERIAL 1 Serial output channel 1 DAC2 Analog output 0 – 5 V channel 2 BIT2 Gate output 0 –...

  • Page 143: Using Multiple Signal Breakout Modules

    Signal Breakout Module Using multiple Signal Breakout Modules More than one Signal Breakout Module can be used to output more than four parameters. Here are instructions for using two modules: 1. Connect the first module between the ERT Control/Gating Module and the PC.

  • Page 144: Signal Breakout Module Data Formats

    WaveGrab is executed on a PC an authorization code is required to activate the software. The PC’s ID code must be transmitted to SA instruments and then an authorization code for that PC can be entered to activate WaveGrab. Revision C, August 2013...

  • Page 145: Capturing Digital Waveform Data From The Serial Output

    ECG, respiration and other waveforms. In that case, a bit from the scanner or another source can be input to the Model 1030 at the AUX BNC connection of the ERT Control/Gating Module. In this case if you check to output the AUX GATE in the bit output and the waveform in the serial output or the BREAKOUT window and if the "include flag bit in the output"...
  • Page 146 Signal Breakout Module To decode the digital waveform data do the following: Read in byte by byte If byte has bit 7 clear, save as low byte If byte has bit 7 set and there was a previous stored low byte Then the 2-byte packet is complete and can be decoded.

  • Page 147: Fiber Optic Universal Gating Module

    Chapter 17 Fiber Optic Universal Gating Module...

  • Page 149: Overview

    Chapter 17 Fiber Optic Universal Gating Module Overview The Fiber Optic Universal Gating Module can be used to supply a gate pulse to imaging systems. It is always used to supply the gate pulse for clinical imaging systems. The module gets an input from the fiber optic AUX GATE OUTPUT of the ERT Control/Gating Module.

  • Page 150: Setup And Use

    Fiber Optic Universal Gating Module Input fiber optic gate light pulse Output 2 mV pulse on RA, LA, RL, LL studs TTL pulse on BNC connector Power switch activates the 9 V internal battery, the power on and low battery LEDs Setup and use When used with a clinical MR system, the Fiber Optic Universal Gating Module is positioned near the patient gantry.

  • Page 151: Micro-Imaging Mr Systems

    Chapter 18 Micro-Imaging MR Systems...

  • Page 153: Overview

    Overview Micro-imaging systems have a vertical bore with very limited space. A special “Micro- Imaging Kit” integrates the Model 1030 Monitoring and Gating System with these MR systems. The Micro-Imaging Kit effectively eliminates RF interference between the Micro-imaging MR system, the Model 1030’s ERT Module and the external environment.

  • Page 154: Micro-Imaging Equipment Setup

    Micro-Imaging MR Systems The ERT Module RF shield and shielded cable in effect place the ERT Module inside the magnet’s RF shield allowing MR images to be free of RF induced interference from the external environment. Micro-Imaging equipment setup The equipment is implemented with the Micro-Imaging MR system as shown above. The ERT Module inside the ERT Module RF Shield resides at any convenient location on the floor under the magnet.

  • Page 155: Bruker Micro 2.5 Mouse And Micro 2.5 Ahs/Rf Imaging Probes

    Micro–Imaging MR Systems Bruker Micro 2.5 Mouse and Micro 2.5 AHS/RF Imaging Probes The Micro 2.5 Mouse probe is shown above with the Lemo and pneumatic respiration connectors mounted in the probe base. Connections for ECG and temperature are made in the probe midsection. There are three ECG pins and a 3 pin Lemo receptacle in the midsection.

  • Page 156: Bruker Mini 0.5 Ahs/Rf Imaging Probe

    Micro-Imaging MR Systems The temperature probe, ECG needle electrodes and rectal temperature probe are shown below in the extended position for connection to the mouse outside the imaging coil. After connecting the sensors to the mouse and inserting the mouse into the coil, excess cable should be taped to eliminate a loop and placed in the cavity in the midsection.

  • Page 157: Bruker Micro-Imaging Kit Components

    Micro–Imaging MR Systems Bruker Micro-Imaging Kit Components The following parts are supplied with the Bruker Micro-Imaging Kit (BMIK-111): Part number Description ERTS-110 ERT Module RF Shield ERTCS-110 Cable, Shielded, Lemo 0B-1B, Micro-Imaging System RMEC-703-19 Subdermal Needle Electrode set for mouse - 19” EGD-705-619 Gold Disk Electrode set, 6mm –...
  • Page 158 Micro-Imaging MR Systems Revision C, August 2013 18-6...

  • Page 159: Water Bed Heater System

    Chapter 19 Water Bed Heater System...
  • Page 160 Revision C, August 2013 19-2...

  • Page 161: Overview

    The MR-compatible Water Bed Heater System is used to control the temperature of small animals undergoing imaging procedures. It can be used with the Model 1030, Model 1025 or Model 1025T Monitoring and Gating Systems or in standalone mode with or without a Fiber Optic Temperature Module.

  • Page 162: Wbh User Interface Window

    Water Bed Heater System WBH USER INTERFACE window Clicking the Water Bed button in the HEATER window opens the WBH USER INTERFACE window. The message area in the upper right of the window gives status and error messages. On the left of the window the user can select the controlled temperature and then enter a temperature set point.

  • Page 163: Test And Service Window

    Water Bed Heater System TEST AND SERVICE window The Test and Service window allows many parameters to be entered which are not useful to the user. However, the window does allow the user to see the two parameters which the control software is setting “flow command” and “PWM count”. The “flow command”...

  • Page 164: Circulation Module Input Output Connections

    Water Bed Heater System Circulation Module input output connections Electrical connections: serial connection to PC Power 12 VDC input power connection Fluid and optic connections: fluid flow to Heater Module for hot COLD fluid flow to Heater Module for cold RETURN fluid flow from Heater Module HEATER IN...

  • Page 165: Heater Module Input Output Connections

    Water Bed Heater System Water Bed Heater Module input output connections Cold water end: input for hot water COLD input for cold water RETURN output for water from the bed CIRCULATOR IN optical input from Circulator Module CIRCULATOR OUT optical output to Circulator Module Warm water end: AC power input power for 100 –...

  • Page 166: Water Beds

    Many animal handling systems have tubes for fluids that can be used as a water bed. Water beds available from SA Instruments include flat and shell beds for mice and rats. Shell beds have the shape of an inverted U and can cover the animal. The beds are inserted in a thin disposable plastic cover before use.
  • Page 167 Water Bed Heater System The Heater Module should be placed as close to the magnet bore as practical. It can be on the SAII MR-compatible instrument stand or on a table. The optimum location will allow the 2 m fluid tubes to connect to a water bed in the bore near the imaging volume. Connections to the Circulation Module A duplex fiber optic cable (10DFOC-23) and the WBH hot, cold and return tube set (WBH3TS-10H/C/R) connect between the Circulation Module and the Water Bed Heater...
  • Page 168 Connections to the Water Bed Connect the heater to bed tubing set to the water bed of choice. Water beds supplied by SA Instruments come with tube fittings that can be inserted in the ends of the tubing set. Energize the Water Bed Heater System Add cold water to the reservoir.

  • Page 169: Turning On The Water Bed Heater Control

    Water Bed Heater System Turning on the Water Bed Heater control With the pumps running, make certain the flow meter is reading between 1.5 and 2.5 gallons per hour. If the flow is less than 1.5 GPH refer to Appendix D. Click the option to control on the Heater Module output temperature.
  • Page 170 Water Bed Heater System Revision C, August 2013 19-10...
  • Page 171 Appendix A Specifications ERT: Range: 40 - 900 BPM ±1% Accuracy: Input range: -2.50 mV to 2.5mV Input Impedance: >10 MΩ @ 10 Hz CMRR: 100 dB @ 60 Hz Resp Range 15 - 300 bpm Accuracy 1 count Sensor pneumatic pillow and/or ECG lead Temp...
  • Page 172 Specifications IBP: Display range 0 – 300 mmHg Channels up to 3 Module Auxiliary inputs 2 analog 0 - 5 V Power 12 VDC & battery Battery life: >6 hours Time to full charge <3 hours Size: hxwxd cm 4.2x15.8x15.0 Pulse Oximetry: Rate Range...
  • Page 173 Specifications Capnograph: Analysis method single beam IR Measurement range end-tidal 0 – 9.9% Accuracy end-tidal 0.15% Measurement range mmHg 0 – 76.0 Accuracy mmHg 1.1 mmHg Sample flow 5 – 20 ml/min Resp Rate 5 – 200 bpm Pneumatic tube length Module Capnometer size hxwxd cm 13x49x41...
  • Page 174 Specifications Revision C, August 2013...
  • Page 175 Appendix B System Components and Accessories Part No. Description M1030OP-rev Operation Manual, Model 1030 SAM & Gating System M1030PCS-rev PC-SAM Software CD 1030-CASE Storage Case Model 1030 System 110100-xx-rev ERT Module ERTBP3-103-PCC ERT Module Battery Pack BPC-210-L ERT Module Battery Pack Cable, L is the length in inches...
  • Page 176 Appendix B: System Components and Accessories Part No. Description 1FTSFOC-23 Simplex Fiber Optic Cable, 1’ long 731100-rev IBP Module IBPX-A IBP Transducer Abbott Transpac IV 750100-rev Simulator SEL-705 Simulator ECG Leads STC-105 Simulator Temperature Cable M9001 MR-compatible Small Animal Air Heater System 761100-rev Air Heater Module 115 VAC 760100-rev...
  • Page 177 System Components and Accessories Part No. Description FOTS-5 Fiber Optic Temperature Sensor, 5’ long, 1 mm tip OD FOTS-9 Fiber Optic Temperature Sensor, 9’ long, 1 mm tip OD FOTE-10 Fiber Optic Temperature Sensor Extension Cable, 10’ long 580100 Pulse Oximeter Module 530100 Fiber Optic Pulse Ox Sensor, 6’...
  • Page 178 Appendix B: System Components and Accessories Revision C, August 2013...

  • Page 179: Appendix C Troubleshooting

    Appendix C Troubleshooting Problem Possible Cause Corrective Action No waveform on Wrong com port Select the correct com port in display. Trace selected. PC-SAM setup menu. frozen & communication error. No waveform on No power to the ERT Check the power and serial display.
  • Page 180 Troubleshooting Problem Possible Cause Corrective Action Heater system has Wrong set point. Check that the set point is cold air blowing from greater than the measured the heater tube. temperature. Heater system has Power is not on. Check power to the Heater cold air blowing from Module.
  • Page 181 Troubleshooting Problem Possible Cause Corrective Action Check power to the Heater Water Bed Heater Power is not on. 19-6 and Circulation Modules. System has no flow Green LEDs indicates power is Water Bed Heater Fiber optic connection Check fiber optic cables for a 19-7 mixed water problem.

  • Page 182: Ert Communication Error

    Troubleshooting ERT communication error This error means the ERT Control/Gating Module is not receiving valid data from the ERT Module. It can occur for one of the following reasons: 1. battery is discharged 2. faulty connection of the Battery Pack Cable at the Battery Pack or at the Module 3.

  • Page 183: No Heat From The Heater

    Troubleshooting No heat from heater If the Heater Module is not generating heat and the yellow “heat” light does not flash, check the following settings: 1. The heater set point is large (70 2. The measured temperature displayed on the PC is less than the set point 3.

  • Page 184: Gating Setup When Large Artifacts Are Present In The Ecg Waveform

    Troubleshooting Module was dropped during shipment, the heat chamber may be damaged. You might start with a cold system and without the Fan and then with the FAN to try to understand what is not working. You can only get very low temperature if one of the following occurs: 1.
  • Page 185 Troubleshooting As an example, consider the case of a cine field echo sequence run on an animal with a heart rate of 600 beats per minute (R-R interval of 100msec). Setting the blanking time to 90 msec will accommodate an arrhythmia rate of up to 10%. If the single slice acquisition time is 10 msec, then 8 slices can be acquired each at a different phase in the cardiac cycle.
  • Page 186 Troubleshooting Revision C, August 2013...

  • Page 187: Appendix D Routine Maintenance

    Appendix D Routine Maintenance Clean the monitor’s surfaces or probes with a soft cotton cloth moistened in a mild soap solution. If disinfection is required, wipe the surface with alcohol, sodium hypochloride or glutaraldehyde (Cidex, Metrucude, etc.). Follow the instructions provided by the manufacturer.
  • Page 188 Routine Maintenance To drain the fluid from the Circulation Module remove the hot, cold and return tubing. Install the hot, cold and return jumper tubing. There are two drain lines one in the front and another in the rear. Most of the fluid can be drained by opening the drain line in the rear of the Circulation Module and turning on the pumps for a few minutes.

  • Page 189: Appendix E Example Waveforms

    Appendix E Example Waveforms This appendix presents the following waveform examples collected in the MR environment: 1. Mouse outside the MR magnet 2. Mouse with E-Resp™ cradle in 7.0 T 3. Mouse with E-Resp™ cradle in 7.0 T, ECG and respiratory gated spin echo sequence Examples 1 and 2 exhibit how important temperature is on the physiology of the mouse and the effect of the strong static magnetic field on the ECG waveform.
  • Page 190 Example Waveforms Mouse outside the MR magnet The ECG waveform was obtained with sub-dermal right arm and left leg electrodes. The measured waveform does not contain signal contributions from respiration or blood flow outside the magnet. Revision C, August 2013...
  • Page 191 Example Waveforms Mouse with E-Resp™ cradle in 7.0 T field The measured ECG waveform has signals superimposed from respiration and blood flow. After the heart contracts (after the R-wave), flowing blood, which is ionic or charged, moving in the strong static magnetic field creates a signal synchronous with the R-wave (i.e.
  • Page 192 Example Waveforms Mouse with E-Resp™ cradle in 7.0 T MR ECG & respiratory gated spin echo sequence This example, which does not show the best way to make gated images, is a good example of observing MR scanner operation from interference in the ECG waveform. The measured ECG waveform has signals superimposed from respiration, blood flow and pulsed magnetic field gradients.

  • Page 193: Warranty, Service And Support

    B. Failure to follow instructions supplied with the product; C. Shipment of the product (claims should be presented to the carrier); D. Repair or attempted repair by anyone not authorized by SA Instruments, Inc. to repair this product; E. Causes other than product defects, including lack of technical skill, competence or experience of the user.
  • Page 194 SA Instruments, Inc. Let the service representative know if you require overnight shipment. 3. In the event estimated repair time is deemed to be excessive by SA Instruments, loaner equipment may be provided. Limitation of liability and exclusion of implied warranties Warranty terms: 1.

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