Cell Test (Chemstation Only) - Agilent Technologies G1315B User Manual

Cell Test (ChemStation only)

The cell test measures the intensity of the deuterium and tungsten lamps over
the full wavelength range (190 – 950 nm), once with the flow cell installed, and
once with the flow cell removed. The resulting intensity ratio is a measure of
the amount of light absorbed by the flow cell. The test can be used to check for
dirty or contaminated flow cell windows. When the test is started, the 1-nm
slit is moved into the light path automatically, and the gain is set to zero. To
eliminate effects due to absorbing solvents, the test should be done with water
in the flow cell.
This test should be performed inititially with a new detector/flow cell. The values should be
N O T E
kept for later reference/comparison.
Cell Test Evaluation
The Agilent ChemStation calculates the intensity ratio automatically. The
intensity ratio (typically between 0.5 and 0.7 for new standard flow cells) is
dependent on the degree of contamination of the flow cell windows, and on
the type of flow cell used.
Figure 45
This test can be used for the standard flow cells only. The nano flow cells will give very low
N O T E
values due to their design.
1200 Series DAD and MWD User Manual
Cell Test Results (report) with no flow cell inserted
Maintenance 8
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