Saving An Image File - Zeiss LSM 800 Standard Operation Protocol

Confocal microscope
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Imaging and Flow Cytometry Core CPOS
G. Saving an Image file
1.
Go to right hand side Image Library column to
find your latest acquired image. Double click on
the image you wish the save. Click on 'Save'
button.
2.
Alternatively,
Right click on the image you wish to save and
then click 'save selected' from the pull down
menu appeared.
3.
Navigate to D:\\ (Hard drive 'D') User\
4.
Create a folder with your PI's name and + 'Lab'
wording suffix. Applicable if no such folder exists
and you are the first one in your lab using LSM
800.
5.
Navigate into the folder you have just created.
6.
Choose file format as '.czi' acronym for 'Carl Zeiss
Image'
7.
Type in name (preferably no spacing).
8.
Click 'Save'
9.
Warning Icon
or changes has been made to your .czi file.
10. Image upload.
Please see guide on Desktop 'Upload guide'.
11. Image Export.
Please see ZEN Blue 2.3 SOP.
12. The following content describes additional
function on the LSM 800 setup.
ver. 2.1 (Updated: 11/13/2020)
LSM 800 SOP
or
indicates unsaved
G2
G-11
G1

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