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ConfoCor 3
9.13
Options
In the major tool group window activate the
Maintain register (Fig. 127). This will display the
Maintain and Options. Open the Options for
ConfoCor menu. The menu has four registers:
−
Data,
−
Measure,
−
LSM + ConfoCor,
−
Auto Save.
9.13.1
Data
9.13.1.1
Saving Raw Data During Measure-
ment
If the Save raw data during measurement
check box is activated (Fig. 128), raw data will be
saved temporarily in a specified directory on disc
(Fig. 129). Each single measurement will be saved
in a single raw data file. The name of the raw data
file is an identifier to the .fcs measurement it
belongs and the channel. This option is used when
access to the raw data is required to analyze the
data in a different way in respect to correlation
and PCH settings or within other programs.
If the check-box is de-activated, no raw data
will be saved.
Temporary raw data will be deleted from the
specified file upon closing the Image FCS
diagram. You will be prompted in the Save
raw data dialogue box, if you want to save
the raw data. Press Yes to save the raw data
permanently along with the .fcs file in a
specified directory, which can be defined in
the Save dialogue box. Press No to discard
the raw data. Note that raw data are saved
along with the .fcs measurement file in the same folder. They are attached to the .fcs file via a
relative file path. If you save the same measurement again without the option Save Raw Data,
than the raw data files will be reassigned an absolute file path and the connection to the .fcs file is
lost.
Please note, if the Auto safe checkbox is ticked in the Auto Save submenu, temporary raw data
will be stored permanently and have to be deleted manually. Care has to be taken to avoid disc
space limitations, since raw data files can be huge.
02/2010
CONFOCOR 3
Options
Fig. 127
Fig. 128
Fig. 129
M60-1-0025 e
ConfoCor Options
ConfoCor Options - Data
Stored temporary raw data
Carl Zeiss
121
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