Agilent Technologies BenchCel User Manual page 111

Workflow for setting sensor thresholds
BenchCel Microplate Handler User Guide
The following factors can affect the reading of the notch sensor and might require that
you adjust the sensor light intensity:
•
Microplate position. For optimum results, the sensor light should bounce off of the
microplate at halfway between the top of the microplate and the top of the plate
skirt.
•
Microplate color. Darker microplate color might require higher light intensity.
•
Microplate material. Shiny or reflective microplates might require lower light
intensity.
•
Microplate condition. Cracks, chips, scratches, or defects in the area where the
sensor light contacts the microplate can affect sensor reading.
•
Ambient light. Brighter rooms might require lower light intensity.
Before you start
Make sure you have three spare microplates for each BenchCel stacker head. For
example, if the BenchCel Microplate Handler has six stacker heads, you should have 18
microplates.
Make a copy of the provided
You can use it to record sensor readings and facilitate threshold calculations.
Workflow
Step For this task...
1 Calculate the Plate-presence
threshold.
2 Determine the optimum sensor
offset at each stack.
3 Calculate the Orientation threshold
based on the observed Notch
Sensors readings.
Overview for setting sensor thresholds
"Worksheet for setting sensor thresholds" on page
See...
"Calculating the Plate presence
threshold" on page 102
"Determining the optimum Orientation
sensor offset" on page 107
"Calculating the Orientation threshold"
on page 111
5 Setting sensor thresholds
113.
101