The Fast Composition Change/Reconditioning Function; Purpose; How The Function Works - Agilent Technologies 1100 Series System Manual

2 Optimizing Performance
Toluene
THF
Water

The Fast Composition Change/Reconditioning Function

Purpose

The capillary pump and the micro well-plate sampler are recommended for capillary LC
applications. Capillary LC methods have very low column flow rates, typically in the
range of 1-20 µl/min. At such low flow rates, re-equilibrating the system to the initial
mobile phase composition between automated gradient runs may require a long time.
To conveniently re-equilibrate the system between automated gradient runs, the Fast
Composition Change/Reconditioning function is implemented.
The Fast Composition Change/Reconditioning function is available only in a system
that includes both a capillary pump and a micro well-plate sampler. This function can
be set up to occur automatically between runs, and/or to occur automatically after any
manual composition change.
NOTE
The Fast Composition Change/Reconditioning function is available only when the
capillary pump is operated in the micro mode.

How the Function Works

Regardless of when it occurs, the Fast Composition Change/ Reconditioning function
is always a 2-step process:
1 The micro well-plate sampler needle is placed over the waste position of the
40 1100 Series Capillary LC System Manual
flushport. The pump delivers a high flow rate at the initial composition defined in
the current method. This flow is maintained for the Fast System Flush time defined
in the user interface. During this time, the system is being re-equilibrated, up to the
sampler needle outlet.
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