Empv Failed To Initialize (Micro Mode Only) - Agilent Technologies 1100 Series System Manual

The analytical column has
become plugged
The filter in front (upstream)
of the EMPV has become
plugged
A component in the micro-
sampler has become
plugged. This could be the
sample loop, needle, needle
seat assembly or injection
valve ports.
A pre-sampler, or post-
sampler capillary in the
system has become plugged,
broken, crushed by a module
cover or overtightened.

EMPV failed to initialize (micro mode only)

Symptoms:
An attempt to pump in the micro mode has resulted in either an EMPV Initialization
Failed error message, or a permanent EMPV Initialization not ready message.
EMPV failed to initialize: Possible Causes and Suggested Actions
1100 Series Capillary LC System Manual
• Replace the column. Otherwise, backflush
the column or replace the column inlet frit.
• Perform a pump purge at 1000 µl/min using
pure water. During the purge, check system
pressure. If pressure is >10 bar, replace the
EMPV filter.
• Using the sampler maintenance positions,
switch the sampler injection valve from
mainpass to bypass. If pressure is significantly
reduced:
a
Backflush or change the needle seat
assembly.
b
c
Replace the needle.
d
Backflush or replace the sample loop
e capillary. Replace the injection valve rotor
seal.
Clean the stator head with acetone, and
make sure the stator head ports are free of
particles.
• Refer to the system flow diagram. One at a
time, disconnect the capillaries in the
following order. When the defective capillary
is found, it may be backflushed with acetone,
or replaced.
a
EMPV-to-flow sensor capillary
b flow sensor-to-sampler injection valve
capillary
c sampler injection valve-to-column inlet
capillary
d flow cell assembly (includes inlet and outlet
capillaries)
55
Basic System Troubleshooting
If a new filter does not reduce the
pressure, replace the mixer.
For severe capillary tube plugs,
acetone is a good backflushing
solvent.
4