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PerkinElmer Lambda 365 Peltier Temp Ctrl Unit Multi Installation Instructions Manual
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PerkinElmer Lambda 365 Peltier Temp Ctrl Unit Multi Installation Instructions Manual

PerkinElmer Lambda 365 Peltier Temp Ctrl Unit Multi Installation Instructions Manual

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This instruction sheet describes the installation of this accessory which is used with the Lambda 365
Spectrophotometer.
Read these instructions before you install this accessory.
NOTE:
Contacting PerkinElmer
Supplies, replacement parts, and accessories can be ordered directly from PerkinElmer, using the part
numbers.
See our website:
http://perkinelmer.com
PerkinElmer's catalog service offers a full selection of high-quality supplies.
To place an order for supplies and many replacement parts, request a free catalog, or ask for
information:
If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be
shipped promptly, usually within 24 hours.
If you are located outside of the U.S., call your local PerkinElmer sales or service office.
Features
•
Full software control
•
Liquid cooling system
•
N
gas purging available
2
Figure 1 Peltier Temp Ctrl Unit Multi w/Ref Heat [P/N: N4101062] or Peltier Temp
Lambda 365 Peltier Temp Ctrl Unit Multi
Installation Instructions
Ctrl Unit Multi no Ref Heat [P/N: N4101063]
09931463A
PerkinElmer, 710
Bridgeport Avenue,
Shelton, CT 06484-
4794, U.S.A
Produced in the USA.

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Summary of Contents for PerkinElmer Lambda 365 Peltier Temp Ctrl Unit Multi

  • Page 1 If you are located within the U.S., call toll free 1-800-762-4000, 8 a.m. to 8 p.m. EST. Your order will be shipped promptly, usually within 24 hours. If you are located outside of the U.S., call your local PerkinElmer sales or service office. Features •...

  • Page 2: Dimensions And Specifications

    09931463A Dimensions and Specifications Peltier Temperature Controller 201 Physical Characteristic Specification Power 100-240 VAC, 50/60 Hz, 380W Temperature Range -5 to 100°C (Maximum Internal Temperature) Maximum Ambient Operating Temperature 40°C 295 (W) x 416 (D) x 283 (H) mm Dimensions (11.6 (W) x 16.4 (D) x 11.1 (H) in) Weight 16.0 kg (35.2 lb)

  • Page 3: Safety Warnings

    09931463A Safety Warnings When this label is attached to an instrument it means refer to the manual. WARNING Lorsque cette étiquette est attachée à un instrument, il est nécessaire de voir le manuel. AVERTISSEMENT There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected.
  • Page 4 09931463A Configuration of Peltier Temp. Ctrl Unit Multi 6-Position Peltier Controlled Cell Changer (with heated reference or without heated reference) Peltier Temperature Controller 201 Power Cord, 3 each Interface Cable (USB), 1 each Spare Fuse (AC 250V T5AL), 2 each Temperature Probe, 1 each Magnetic Stirrer, 6 each Coolant Hose, 1 each...
  • Page 5 09931463A Figure 2-2 Lambda 365 6-Position Peltier Controlled Cell Changer (without heated reference) a. Interface connector between 6-Position Peltier Controlled Cell Changerand Peltier Temperature Controller 201 b. N Gas fitting c. Coolant Inlet/Outlet Quick Coupler d. Interface slot for Temperature Probe e.
  • Page 6 09931463A Peltier Temperature Controller 201 Front View of Peltier Temperature Controller 201 Figure 6 Front view a. Flow Gauge: Indicator of the flow rate of N2 gas b. Coolant Inlet c. LED Indicators: Display the status of the operation of Coolant Circulation, Fan, Pump, etc. (If there is any problem in the components, Red LED will flash with beep alarm.).
  • Page 7 09931463A Rear View of Peltier Temperature Controller 201 Figure 7 Rear view a. Air Vent Manual button: It is used to identify the cause of alarm that sounds when the trouble occurs with Peltier. The cause of the alarm is as follows; When the air occur in the coolant hose.
  • Page 8 09931463A How to Fill or Drain the coolant How to Fill the coolant When the Peltier Temp Ctrl Unit Sgl is installed for the first time, the enclosed Coolant should be filled as NOTE: the following procedures. Prepare the Peltier Temperature Controller 201 in a location that is compatible with the required environmental conditions for the operation.
  • Page 9 09931463A Push the Air Vent Manual Button on. Figure 11 Push the button Turn on the AC power switch of the Peltier Temperature Controller 201. Figure 12 Turn on the AC power switch Check if the LEDs of LOW LEVEL and FLOW SENSOR are lit on. Figure 13 LEDs of LOW LEVEL and FLOW SENSOR...
  • Page 10 09931463A Open the lid of the coolant inlet on top of the Peltier Temperature Controller 201 and fill up the liquid coolant using the funnel. Figure 14 Lid of coolant inlet Keep the amount of liquid coolant to level where the indicator is located between the ‘L’ (low) and ‘H’...
  • Page 11 09931463A After filling up the coolant, check the ‘LOW LEVEL’ LED is not lit on. Figure 16 Coolant level Close the lid of the coolant inlet. Turn off the AC power switch of the Peltier Temperature Controller 201. Push the Air Vent Manual button off. Fasten Phillips screws (4 each) on the Air Vent Manual button cover using Phillips screwdriver.
  • Page 12 09931463A Prepare a waste basket. Figure 19 Waste basket Turn on the AC power switch of the Peltier Temperature Controller 201. Figure 20 Turn on the AC power switch Connect the waste hose to the Coolant Drain port on the rear panel. Figure 21 Connect the waste hose The Coolant flows out as soon as the waste hose is connected, so the other side of the waste hose should NOTE:...

  • Page 13: Installation

    09931463A If the coolant falls below ‘L’ (Low level), the LEDs of LOW LEVEL and FLOW SENSOR are lit on. Figure 22 LEDs of LOW LEVEL and FLOW SENSOR When drain is completed, disconnect the waste hose. 10. Turn off the AC power switch of the Peltier Temperature Controller 201. 11.
  • Page 14 09931463A Pull out the cell holder and base plate by hand. Figure 24 Pulling out the cell holder and base plate Insert the 6-Position Peltier Controlled Cell Changerin the sample compartment. Figure 25 Inserting the 6-Position Peltier Controlled Cell Changer Gently press the 6-Position Peltier Controlled Cell Changer to connect the communication port (male) under the bottom of the 6-Position Peltier Controlled Cell Changer to the port (female) in the sample compartment.
  • Page 15 09931463A Fix and tighten the 6-Position Peltier Controlled Cell Changerwith the Phillips round head screws. Figure 27 Location of screws (with heated reference or without heated reference) Connect the accessory interface cable of the Peltier Temperature Controller 201 to the interface connector of the 6-Position Peltier Controlled Cell Changer.
  • Page 16 09931463A Connect the coolant inlet/outlet and N gas tube between the Peltier Temperature Controller 201 and the 6-Position Peltier Controlled Cell Changer. Figure 30 Connecting the coolant inlet/outlet and N gas fitting gas is not always required. If no gas is applied, user does not have to connect N gas tube.
  • Page 17 09931463A Stuff the probe line into the cell compartment and close the lid of the Interface slot for temperature probe. Figure 33 Stuffing the probe line into the cell compartment Connect the Peltier Temperature Controller 201 with the PC via the USB cable. Figure 34 Connecting the Peltier Temperature Controller 201 with the PC Connect the power cord to the Peltier Temperature Controller 201.
  • Page 18 09931463A Turn on the AC power switch of the Lambda 365 and the Peltier Temperature Controller 201. The air vent manual button should be off before turning on the main power of the CAUTION Peltier Temperature Controller 201 and also during the operation since it could make a trouble for the communication between Lambda 365 and Peltier Temperature Controller 201.
  • Page 19 09931463A Setting USB Serial Port When using the USB cable, USB Driver has already been installed when installing the Lambda 365 and UV NOTE: Express software, user does not need to install it again. When the communication by the USB is not established, change the port setting as follows; 1.
  • Page 20 09931463A Select Ports (COM & LPT) to expand the listing. These are the devices currently connected to the COM ports. USB Serial Port (COMx) is listed when the driver installation is completed successfully. Double click on the USB Serial Port (COMx) of the Ports (COM & LPT). 5.
  • Page 21 09931463A Change the parameter values as shown below. Select OK after checking the changed parameter values. If the Peltier Temperature Controller 201 fails to communicate with the PC, change the COM Port Number by the following steps. Open Advanced Setting for COMx window again by repeating the steps 1 to 6.
  • Page 22 09931463A Select the COM Port number list to expand it and change the COM port number to another one which is not in use from COM 1 to COM 10. 11. Make sure that the changed COM Port Number is applied, and select OK. 12.
  • Page 23 09931463A Measurement Thermal Denaturation Mode Install the 6-Position Peltier Controlled Cell Changer referring to section Installation (page 13). Close the sample compartment cover and turn on the instrument. Before System Self Test is performed, the 6-Position Peltier Controlled Cell Changer should be installed. NOTE: Otherwise, the instrument can be damaged electrically and do not operate properly.
  • Page 24 09931463A a. Y Unit: Select one of the display unit : Absorbance, Transmittance, Reflectance or Energy. b. SBW (nm): Select bandwidth. There are five bandwidths selectable: 0.5, 1, 2, 5 or 20 nm. c. Tm Method: Select a method for determining melting temperature. Options include: 1st derivative and Average.
  • Page 25 09931463A Select Connection Check, then Method Setup will be activated. Select Method Setup. NOTE: Check the communication between Computer and Peltier Temperature Controller 201, see section Setting USB Serial Port (page 19) if the Method Setup is not activated. The Peltier window will be shown. Select the Multi Cell Peltier tab and set parameters according to the experiment conditions.
  • Page 26 09931463A b. Using Cells: Show the cell positions which are selected for measurement. Remove the cells by and switch positions by keys. c. Multi Zero: If checked, zero will be measured all the selected positions. If not, zero will be only measured at the 1 cell position among the using cells.
  • Page 27 09931463A After completing the parameter setup, select Apply and OK. Then the LED for ON-LINE is turned on, and it will start heating up or cooling down to the Start temperature in the Multicell Peltier tab. Insert blank solutions into the both reference and sample cell holder. Select Baseline and then baseline will be measured when it reaches the set Start Temperature.
  • Page 28 09931463A g. Error Range (°C): It shows the temperature tolerance between the sampling and monitored temperature. The measurement will start when the temperature tolerance reaches within the set Error Range. h. Hold Time: It shows the set holding time. i. Elapsed time (s): It shows the elapsed time to reach the sampling temperature. j.
  • Page 29 09931463A When the 6-Position Peltier Controlled Cell Changer is connected for the first time, you need to correct NOTE: the cell position. Calibrate the beam position of the 6-Position Peltier Controlled Cell Changer, referring to the see section Calibration of Multi-Cell Position (page 33). 6.
  • Page 30 09931463A Check the communication between Computer and Peltier Temperature Controller 201, see section NOTE: Setting USB Serial Port (page19) if the Method Setup is not activated. 9. The Peltier window will be shown. Select the Multi Cell Peltier tab and set parameters according to the experiment conditions.
  • Page 31 09931463A ▶ Temperature Display g. Select which temperature will be displayed on the panel:Block, Probe 1 or Probe 2. The only selected temprature is displayed in real time. h. Offset: This function is used to adjust temperature. It is only used for manfacturing process, so do not modify the values.
  • Page 32 09931463A Start Temp (°C): The starting temperature of experiment. SP Temp (°C): The set temperature to go to the next measurement temp. Block Temp (°C): The temperature of Cell Block. Probe 1 Temp (°C): The temperature of Probe 1. Probe 2 Temp (°C): The temperature of Probe 2. Monitor: Selected temperature is being monitored.
  • Page 33 09931463A Calibration of Multi-Cell Position Calibrate the beam position of the 6-Position Peltier Controlled Cell Changerwhen the Multi-Cell is NOTE: installed for the first time or beam position is incorrect. 1. Execute one measurement mode of UV Express software (Scan, for example) and open the Method Setup of the Multi-Cell Peltier.
  • Page 34 09931463A 3. The Functions of the Multi-Cell Calibration are shown as follows. Command Function MultiCell Position It shows saved steps of each cell position of the Multi-Cell. It is used for moving Multi-Cell position as clicking buttons Multicell Move It is used for moving Multi-Cell position using buttons by entered step.
  • Page 35 09931463A 6. Then the Multi-Cell calibration will start. The current process of calibration will be shown in the main window. 7. When calibration is finished, the following box appears. Click OK. 8. Click Save Result to save the result. If the following message box appears, click OK.
  • Page 36 09931463A Peltier Temperature Controller 201 Auto Tuning Setup Peltier Temperature Controller 201can be compatible with various peltier cell holders (Single or Multi). NOTE: Whenever you exchange the existing peltier cell holder to another one, you should perform the “AUTO TUNING” set up to minimize the temperature fluctuation at the target temperature. 1.

  • Page 37: Troubleshooting

    09931463A Troubleshooting Power LED is not lit on 1. Check the connection of the power cord or the fuse. The fuse is located at the rear of the instrument. There is risk of receiving a fatal electric shock if the fuses are replaced with the power cord connected.
  • Page 38 09931463A FAN LED is lit on with an alarm sound 1. Fan needs to be replaced. LED of LOW LEVEL, FLOW SENSOR and PUMP blinks with an alarm sound 1. Tunn off the power of the peltier temperature controller 201. 2.
  • Page 39 09931463A 5. Check if the LED blinks of LOW LEVEL, FLOW SENSOR and PUMP stop. 6. Close the lid of the coolant inlet. 7. Turn off the AC power switch of the Peltier Temperature Controller 201. 8. Push the Air Vent Manual button off. 9.
  • Page 40 9. Push the Air Vent Manual button off. 10. Fasten Phillips screws (4 each) on the Air Vent Manual button cover using Phillips screwdriver. 11. If the FLOW SENSOR and PUMP LED is continuously on, contact your PerkinElmer Service representative.
  • Page 41 1. Recalibrate if the light beam does not reach the center of the cell holder’s hole. 2. If the intensity value is still low after the recalibration, the lamps of the light source may be deteriorated and need replacing. Contact your PerkinElmer service representative. When 6-Position Peltier Controlled Cell Changerdoes not reset 1.
  • Page 42 09931463A...