Nikon Epiphot 300 Manual page 74

Symptoms
Causes
Dust
• Dust on large format
imprinted
mirror.
on large
format film.
• Numerical aperture
too low.
Insufficient
resolution.
• Photo enlarged
excessively.
• Light enters through
binocular eyepieces.
Ghost or
flare.
• Stray light enters.
• Use of filters
inappropriate.
• Inappropriate film used
(its emulsion
inappropriate).
Unable to
perform
good color
• Light source voltage
photo-
not as specified.
micrograpy.
• Inadequate exposure
time (failure in film
reciprocy).
• Influence of
developing process.
Control box
• Fuse blown.
doesn't
work even
though its
• No electricity.
power is
turned on.
Control box
• Specified fuse not
fuse easily
used.
blows.
• Vacuum in the dusts using supplied plate and vacuum
cleaner. (P. 70, 71)
• Use objective with high numerical aperture.
• To obtain identical total magnification, use of higher
magnification objective gives higher resolution and
contrast to the image.
• Upper limit of effective total magnification is between
500 and 1000 times of objective's numerical aperture.
Photograph blurs if taken beyond this range.
• Darken surrounding environment or cover eyepieces
with caps.
• Do not expose microscope or sample to hazardous,
strong light such as direct sunlight.
• Use NCB11 filter. (P. 56)
• Spectral sensitivity of daylight-type film differs
also
considerably from type to type or from manufacturer to
manufacturer.
• Varieties of film of identical brand often exhibit
different color development due to difference in
emulsion used. Note emulsion number of each film for
such difference.
• Perform photomicrography at specified voltage. (P. 60)
• Color development is adversely affected by inadequate
exposure time as it causes failure in film reciprocy.
Use an exposure time display meter and ND filters to
adjust exposure time which suits film characteristics.
Or use color compensation filters to compensate for
film reciprocy failure. (Refer to Data Sheet on the film.)
• It is a good idea to consult with developing house
about film characteristics when preparing color prints.
• Replace fuse.
• Connect power cable to AC IN line receptable.
• Use 1N250 V fuse.
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