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4. Simplified Brightfield Polarization l\1icroscopy and
Sensitive Brightfield Polarization l\licroscopy
(1) Push the mirror block changeover lever to the click stop to put the brightfield mirror
block (with B.F. indication) in the optical path.
(2) Mount the polarizer slider with the first order red compensator and the analyzer slider
in their respective ports. But keep them out of the optical path.
(3) If any Nomarski prism has been attached to the revolving nosepiece, remove them.
( 4) Turn on the power switch of the power supply unit. Rotate its lamp intensity control
dial to CTRL. Set the lamp intensity control dial of the microscope base at about 9V.
(5) Pull the anti-glare ND8 filter insertion/removal lever to remove this filter from the
optical path.
(6) Put the NCB 11 filter in the optical path.
Push both the OB/PHOTO•SIDE and OB/PHOTO changeover levers to provide the
(7)
binocular eyepieces with 100% light output.
(8) Rotate the coarse focus knob to lower the revolving nosepiece. Rotate the nosepiece
to put the 1 Ox objective in the optical path.
(9) Place a sample on the stage facing its side to be observed downward. View the sample
through the binocular eyepieces, and while so doing, rotate the coarse focus and fine
focus knobs to focus on the sample.
(10) Switch to the desired objective to focus on the sample.
►
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• Nikon's 50x or higher magnification objectives (except ELWD) are
• When the stage is moved laterally or longitudinally, some of the
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equipped with a safety mechanism. However, the top lens of each of
these objectives slightly protrudes beyond the metal rim around it.
When rotating the revolving nosepiece to which these high
magnification objectives have been mounted, pay close attention so as
not to hit them against the sample or the like.
objectives mounted to the revolving nosepiece may hit against the
metal bottom plate of the stage. Carefully rotate the nosepiece so as
not to hit such objectives against the metal parts
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