Nikon Epiphot 300 Manual page 35

(9) Place a sample on the stage. Face its surface to be observed downward. View the
sample through the binocular eyepieces. While so doing, rotate the coarse focus and
fine focus knobs to focus on the sample.
It is also possible to change over to darkfield microscopy after focusing the sample
during brightfield microscopy. (See "Changing over between Brightfield and
Darkfield Microscopy" written below)
(10) Switch to the desired objective and focus on the sample.
►
• Nikon's SOx or higher magnification objectives (except ELWD) are
equipped with a safety mechanism. However, the top lens of each of
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these objectives slightly protrudes beyond the metal rim around it.
When rotating the revolving nosepiece to which these high
magnification objectives have been mounted, pay close attention so
as not to hit them against the sample or the like.
• When the stage is moved laterally or longitudinally, some of the
objectives mounted to the revolving nosepiece may hit against the
metal bottom plate of the stage. Carefully rotate the nosepiece so as
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not to hit such objectives against the metal parts.
(11) Adjust the brightness with ND filters and the lamp intensity control dial. *
When the quintuple brightfield revolving nosepiece is used, it is impossible to perform dark.field
microscopy.
*
Changing over between Brightfield and Darkfield Microscopy
(1) Push the anti-glare ND8 filter insertion/removal lever to put this filter in the optical path.
(2) Changeover from brightfield to darkfield microscopy
G) Pull the mirror block changeover lever to put the darkfield mirror block (with D.F. indication) in
the optical path. As this changeover lever is pulled out, the field diaphragm is fully opened.
® Fully open the aperture diaphragm.
G) Adjust the brightness with ND filters and the lamp intensity control dial.
(3) Changeover from darkfield to brightfield microscopy
G) Push the mirror block changeover lever to put the brightfield mirror block (with B.F. indication) in
the optical path. As this changeover lever is pushed, the fully opened field diaphragm is stopped
down as set by the field diaphragm dial.
► �--------------------------------�
1 When removing the darkfield mirror block from the optical path, keep I
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: your eyes off the binocular eyepieces as glare may enter the viewfield.
®
Tum the aperture diaphragm dial to open or stop down this diaphragm properly.
G) Adjust the brightness with ND filters and the lamp intensity control dial.
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