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3. Differential Interference Contrast l\licroscopy
(1) Push the mirror block changeover lever to the click stop to put the brightfield mirror
block (with B.F.indication) in the optical path.
(2) Mount the polarizer slider with the first order red compensator and the analyzer slider
in their respective ports. But keep them out of the optical path.
(3) Turn on the power switch of the power supply unit. Rotate its lamp intensity control
dial to CTRL. Set the lamp intensity control dial of the microscope base at about 9V.
(4) Pull the anti-glare ND8 filter insertion/removal
lever to remove this filter from the optical path.
(5) Put the NCBll filter in the optical path.
(6) Push both the OB/PHOTO· SIDE and OB/PHOTO
changeover levers to provide the binocular
eyepieces with 100% light output.
(7) Rotate the coarse focus knob to lower the revolving nosepiece. Rotate the nosepiece
to put the l0x objective in the optical path.
(8) Place a sample on the stage. Face its side to be observed downward. View the sample
through the binocular eyepieces. While so doing, rotate the coarse focus and fine
focus knobs to focus on the sample.
(9) Switch to the desired objective and focus on the sample.
►
I• Nikon's SOx or higher magnification objectives (except ELWD) are
1
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• When the stage is moved laterally or longitudinally, some of the
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( 10) Tum the aperture and field diaphragm dials to open or stop down these two
diaphragms.
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equipped with a safety mechanism. However, the top lens of each of
these objectives slightly protrudes beyond the metal rim around it.
When rotating the revolving nosepiece to which these high
magnification objectives have been mounted, pay close attention so as
not to hit them against the sample or the like.
objectives mounted to the revolving nosepiece may hit the metal
bottom plate of the stage. Carefully rotate the nosepiece so as not to
hit such objectives against the metal parts.
NDB filter insertion/
removal lever
•
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