3M Petrifilm 6447 Product Instructions page 5

Scope of Validation:
For the following matrices or categories stainless steel, ceramic tile, sealed concrete.
Incubation:
Incubate the 3M Petrifilm EL Plates 28 hours ± 2 hours at 35ºC ± 1ºC or 37ºC ± 1ºC.
References
1. U.S. Food and Drug Administration. Code of Federal Regulations, Title 21, Part 58. Good Laboratory Practice for
Nonclinical Laboratory Studies.
2. ISO 7218. Microbiology of food and animal feeding stuffs – General requirements and guidance for microbiological
examinations.
3. ISO/IEC 17025. General requirements for the competence of testing and calibration laboratories.
4. Atlas, Ronald M. 1993. Handbook of Microbiological Media, CRC Press.
5. International Standards Organization, ISO 11290-2:1998. Microbiology of food and animal feeding stuffs –
Horizontal method for the detection and enumeration of Listeria monocytogenes – Part 2: Enumeration method.
Refer to the current versions of the standard methods listed above.
Explanation of Symbols
www.3m.com/foodsafety/symbols
AOAC is a registered trademark of AOAC INTERNATIONAL.
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