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Operation
(continued)
Phase Contrast Observation
Depending on the configuration ordered, the EXI-410 can be used for phase contrast observation with
LWD phase contrast objectives: 4x, 10x, 20x and 40x.
For phase contrast observation, replace the normal objectives with phase contrast objectives on the
nosepiece – refer to page 8 for objective installation instructions. Brightfield observation can still be
performed with phase contrast objectives, but phase contrast observation requires phase contrast
objectives.
Phase Contrast Slider
The adjustable phase slider is pre-aligned at our facility, so
further adjustment is not typically necessary. If the phase
ring is not centered, you can adjust it by centering the bolt
with the 2mm hex wrench provided with the microscope –
see instructions below.
The EXI-410-PH includes a 3-position phase slider.
Position 1 is for the 4x objective; Position 2 is for the
10x/20x/40x objectives. Position 3 is "open" for use with
optional filters.
Match the 4x and 10x/20x/40x light annuli with phase
contrast objectives of matching magnifications.
Installing the Phase Slider (Optional) (Refer to Page 14)
Centering the Light Annulus
The phase slider is pre-aligned at our facilities. If
realignment is necessary, follow these steps:
1. Place a specimen on the stage and bring it into focus.
2. Replace the eyepiece in the eyepiece tube with the
centering telescope (optional).
3. Make sure the magnification of the objective in the light
path matches that of the light annulus on the phase
slider.
ACCU-SCOPE
®
73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com
EXI-410 INVERTED MICROSCOPE SERIES
Annular diaphragms
(phase annuli)
Open position
22
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