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EXI-310 INVERTED MICROSCOPE SERIES
Operation
(continued)
Using the Aperture Diaphragm (Fig. 12)
70-80%
The iris diaphragm determines the numerical aperture
(N.A.) of the illumination system in bright field
observation. When the N.A. of the objective and the
30-20%
illumination system match, you can obtain higher
image resolution and contrast, as well as an
increased depth of focus.
To check the iris diaphragm: remove the eyepiece
and insert the centering telescope (if you purchased
one). When observing through the eyepiece, you will
see the field of view as shown in Fig. 13. Adjust the
iris diaphragm lever to the desired contrast.
When observing a dyed specimen, set the iris
Fig.24
Fig. 12
diaphragm ② to 70-80% of the N.A. of the objective
① in use. However, when observing a culture
specimen, which is not dyed, set the iris diaphragm
lever to 75% of the N.A. of the objective in use.
Phase Contrast Observation
Depending on the configuration ordered, the EXI-310
can be used for phase contrast observation with LWD
phase contrast objectives: 4x, 10x, 20x and 40x.
For phase contrast observation, replace the normal
objectives with phase contrast objectives on the
nosepiece – refer to page 8 for objective installation
instructions.
Fig. 13
Phase Contrast Slider (Fig. 13)
The adjustable phase slider is centered at our
facilities so no further adjustment should be
necessary. If the phase ring is not centered, you can
adjust it by centering the bolt with the 2mm hex
wrench provided with the microscope – see
instructions on page 14.
The EXI-310-PH includes a 3-position phase slider.
Position 1 is for the 4x/10x objectives; Position 2 is for
the 20x/40x objectives. Position 3 is "open" for use
with optional filters.
Match the 4x/10x or 20x/40x light annulus with phase
contrast objectives.
®
ACCU-SCOPE
13
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