Accu-Scope EXI-410 Series Manual

Accu-Scope EXI-410 Series Manual

Inverted microscope series
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MANUAL
EXI-410
INVERTED
MICROSCOPE SERIES
73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • 631-543-8900 (F)
www.accu-scope.com • info@accu-scope.com

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Summary of Contents for Accu-Scope EXI-410 Series

  • Page 1 MANUAL EXI-410 INVERTED MICROSCOPE SERIES 73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • 631-543-8900 (F) www.accu-scope.com • info@accu-scope.com...
  • Page 3: Table Of Contents

    EXI-410 INVERTED MICROSCOPE SERIES CONTENTS SAFETY NOTES ........................3 CARE AND MAINTENANCE ....................4 INTRODUCTION ........................5 UNPACKING AND COMPONENTS ..................5 COMPONENT DIAGRAMS ....................6 MICROSCOPE DIMENSIONS.................... 12 ASSEMBLY DIAGRAM ....................... 13 DETAILED ASSEMBLY ...................... 14 OPERATION POWERING ON THE MICROSCOPE ..................18 ILLUMINATION .........................18 INTERPUPILLARY DISTANCE ....................18 FOCUSING ..........................19...
  • Page 4: Safety Notes

    Do not grasp or hold using any other parts (such as the illumination pillar, focus knobs, eyetubes or stage) when carrying the microscope. Doing so may result in dropping the unit, damage to the microscope or failure of proper operation. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 5: Care And Maintenance

    An annual schedule of preventative maintenance by qualified personnel is highly recommended. Your authorized ACCU-SCOPE distributor can arrange for this service. ® ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 6: Introduction

    9. Fluorescence filter cubes (optional) Optional accessories such as optional objectives and/or eyepieces, slides sets, etc., are not shipped as part of the standard equipment. These items, if ordered, are shipped separately. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 7: Component Diagrams

    17. Mechanical Stage Control Knobs (XY movement) 8. Main Frame 18. Focus Tension Adjustment Collar 9. LED Lamp (transmitted) 19. Coarse Focus 10. Illumination Pillar 20. Fine Focus 11. Condenser Set Screw ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 8 5. Mechanical Stage with Universal 13. Camera Port Holder (optional) 14. Power Indicator 6. Objective 15. Illumination Selector 7. Nosepiece 16. Illumination Intensity Adjustment Knob 8. Power Switch ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 9 12. Rear Hand Grasp 5. Focus Tension Adjustment Collar 13. Mechanical Stage (optional) 6. Coarse Focus 14. Nosepiece 7. Fine Focus 15. Fuse 8. Condenser Set Screw 16. Power Outlet ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 10 22. Mechanical Stage Control Knobs (XY movement) 11. Illumination Pillar 23. Tension Adjustment Collar 12. Condenser Set Screw 24. Coarse Focus 13. Field Iris Diaphragm 25. Fine Focus ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 11 15. Camera Port Holder (optional) 6. Objective 16. Power Indicator 7. Nosepiece 17. Illumination Selector 8. Fluorescence Turret 18. Illumination Intensity Adjustment Knob 9. Fluorescence Turret Access Door ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 12 14. Mechanical Stage (optional) 6. Coarse Focus 15. Nosepiece 7. Fine Focus 16. LED Fluorescence Light Source 8. Condenser Set Screw 17. Fuse 9. Phase Contrast Slider 18. Power Outlet ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 13: Microscope Dimensions

    EXI-410 INVERTED MICROSCOPE SERIES MICROSCOPE DIMENSIONS EXI-410 Phase Contrast and Brightfield EXI-410-FL with Mechanical Stage ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 14: Assembly Diagram

    When assembling the microscope, make sure that all parts are free of dust and dirt, and avoid scratching any parts or touching glass surfaces. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 15: Detailed Assembly

    Align the two screw holes on the universal holder plate with the set screws on the lateral movement ruler of the mechanical stage. Tighten the two set screws. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 16 The clear glass allows you to view the objective in position. Eyepieces ① Remove the eyetube plugs and fully insert the eyepieces ② ① into the eyepiece tubes ②. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 17 “click” is heard. 4. Replace the filter turret cover. NOTE: ● Fluorescence filter sets must match the fluorescence LED excitation light source and the fluorescence probes used in the application. Please contact with ACCU-SCOPE with any questions about compatibility. ACCU-SCOPE ®...
  • Page 18 Insert the power plug into the power outlet of the microscope; make sure the connection is snug. Plug the power cord into a power supply receptacle. NOTE: Always use the power cord that came with your microscope. If your power cord becomes damaged or lost, please call your authorized ACCU-SCOPE dealer for a replacement. ACCU-SCOPE ®...
  • Page 19: Operation

    Where the “●” ① on the eyepiece tube lines up, that is the number for your interpupillary distance. The range is 54- 75mm. Make note of your interpupillary number for future operation. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 20: Focusing

    Focus travel: The default focus travel from the surface of the plain stage is up 7mm and down 1.5mm. The limit can be increased up to 18.5mm by adjusting the limit screw. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 21: Adjustable Tension Control

    60mm Petri dish/microscope slide holder ② mounted in the universal holder of the mechanical stage. The specimen holder can then be moved by turning the X③ and Y④ stage movement controls. ③ ④ ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 22: Selecting The Light Path

    NOTE: An iris diaphragm that is closed too far will give optical artifacts in the image. An iris diaphragm that is too open may make the image appear too “washed out”. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 23: Phase Contrast

    2. Replace the eyepiece in the eyepiece tube with the centering telescope (optional). 3. Make sure the magnification of the objective in the light path matches that of the light annulus on the phase slider. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 24: Emboss Contrast

    Emboss contrast microscopy requires a condenser-side emboss contrast slider and eyepiece- tube-side emboss contrast slider. These were shipped with the microscope and the installation and operation instructions are below. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 25: Using A Microscopy Camera (Optional)

    ❶. Using a Microscopy Camera (Optional) Installing Couplers (Refer to Page 16) Selecting the Light Path for Observation/Imaging with a Camera (Refer to Page 21) ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 26: Using Fluorescence (Optional)

    Various filter sets are also available for installation. Selection of filter sets depends upon the available LED fluorescence modules in your microscope. Contact your authorized ACCU-SCOPE dealer, or call us at 631- 864-1000 for a list of available and recommended filter sets.
  • Page 27 NOTE: a UV light shield is included with the EXI-410-FL version to reduce extraneous light from the fluorescence sample. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 28: Troubleshooting

    Details are indistinct  The light annulus and the phase annulus are Adjust the centering screws to center it. not centered. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 29 Use n designated lamp. The light intensity is not bright The brightness adjustment knob is not Adjust the brightness adjustment knob in a enough adjusted properly. correct way. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 30 The indoor window or the The stray light enters through the eyepieces Cap/cover both the eyepieces before fluorescence lamp is imaged. and is reflected to the camera. imaging. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
  • Page 31: Maintenance

    ACCU-SCOPE INC. This warranty expressly excludes any liability by ACCU-SCOPE INC. for consequential loss or damage on any grounds, such as (but not limited to) the non-availability to the End User of the product(s) under warranty or the need to repair work processes. Should any defect in material, workmanship or electronic component occur under this warranty contact your ACCU-SCOPE distributor or ACCU-SCOPE at (631) 864-1000.

This manual is also suitable for:

Exi-410-flExi-410-ph

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