Related Manuals for Accu-Scope EXI-600
Summary of Contents for Accu-Scope EXI-600
- Page 1 MANUAL EXI-600 INVERTED RESEARCH MICROSCOPE SERIES 73 Mall Drive, Commack, NY 11725 • 631-864-1000 (P) • 631-543-8900 (F) www.accu-scope.com • info@accu-scope.com...
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Page 2: Table Of Contents
CONTENTS ATTENTION ............................ 4 SAFETY PRECAUTIONS ....................... 5 COMPONENT IDENTIFICATION ....................7 APPLICATIONS ..........................8 ASSEMBLY ............................. 9 5.1. PREPARATION BEFORE ASSEMBLY .................. 9 5.2. ASSEMBLY ........................... 10 5.2.1. Trinocular observation tube ................... 10 5.2.2. Eyepieces ........................11 5.2.3. Transmitted illumination pillar..................11 5.2.4. - Page 3 EXI-600 FLUORESCENCE CONFIGURATION DIAGRAM ............42 TROUBLESHOOTING ......................43 10.1. Optical ........................... 43 10.2. Electrical ..........................44 10.3. MICROSCOPY IMAGING ..................... 45 MAINTENANCE........................46 LIMITED MICROSCOPE WARRANTY ..................47 ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 4: Attention
EXI-600 INVERTED MICROSCOPE SERIES 1. ATTENTION This manual is written for the EXI-600 research inverted biological microscope. To ensure the safety, obtain optimum performance and to familiarize yourself fully with the microscope, it is strongly recommended that you read this manual carefully before operating the microscope and put this manual in a place easy to access for reference. -
Page 5: Safety Precautions
Allow the lamp house to cool completely before covering the microscope with the dust cover. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... - Page 6 The protection offered by the instrument’s safety features may decrease or be nullified if the device is tampered with or not used as prescribed in this manual. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 7: Component Identification
25. Transmitted illumination pillar 12. Right coarse/fine focusing knob 26. LED lamp house 13. Magnification changer turret 27. Filter holders 14. Right camera port 28. Transmitted light field diaphragm ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 8: Applications
Upper light path selector 4. APPLICATIONS The EXI-600 inverted research microscope is primarily used to study living cells growing in tissue-culture dishes. The microscope can be configured with a variety of contrast techniques to enable observation, imaging and analysis such as brightfield (standard), phase contrast, differential interference contrast (“DIC”), and fluorescence, and is an ideal platform for integration with 3rd party accessories such as LED-... -
Page 9: Assembly
EXI-600 INVERTED MICROSCOPE SERIES 5. ASSEMBLY The EXI-600 will arrive partially assembled. Some parts and components may need to be installed and aligned before use and for optimal performance. PREPARATION BEFORE ASSEMBLY 5.1. Tear down the packaging of the microscope frame and the accessories. Packaging includes the main frame, eyepieces, objectives, condenser, LED lamp house, stage, and other accessories such as filters, DIC blocks, dust-proof shield, tools (optional) and user manual. -
Page 10: Assembly
A slot is built into the mainframe through which the 2mm hex screwdriver will fit to tighten the 2mm hex screw. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 11: Eyepieces
Secure the pillar onto the frame using the same 4 hex screws from above. 4mm hex screws When installed properly, the pillar can be easily tilted backward and forward with one hand. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 12: Transmitted Led Lamphouse
EXI-600 INVERTED MICROSCOPE SERIES 5.2.4. Transmitted LED Lamphouse NOTE: The EXI-600 microscope typically ships with the transmitted LED lamphouse already installed. The instructions below may be useful for maintenance or replacement. Insert the dovetail of the lamp house into the socket and ensure... -
Page 13: Stage
Hand tight is sufficient. Repeat until all objectives are installed. To minimize dust from getting on the optics below, leave dust plugs in place where objectives are not installed. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 14: Stage Inserts
Spring Only one stage insert can be installed at a time, and this procedure allows for easy exchange of inserts based on need. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 15: Turret Condenser
Ensure Condenser the dovetail is completely inserted centering screw into the carrier. Secure the condenser by tightening Alignment the lock screw. Dovetail groove Lock screw Pillar Condenser ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 16: Differential Interference Contrast (Dic): Component Installation
DIC components to ensure they are free of dust and fingerprints. Installation of DIC condenser prisms should be performed ONLY by ACCU-SCOPE-authorized service technicians. The instructions below are for informational purposes only. Insert or exchange a DIC prism... - Page 17 DIC nosepiece be used for DIC observation. prism Insert the DIC nosepiece prism DIC nosepiece that corresponds to the objective Dust plug prism installed magnification. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 18: Fluorescence Filter Cube Turret (Optional)
To insert the cube turret into the frame, turn the locking lever down. Raise the cover to reveal the location into which the turret will be inserted. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 19: Fluorescence Light Source (Optional)
Using a 4mm hex screwdriver, insert and tighten the provided hex screws to secure the shield in place. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 20: Fluorescence Sliders (Optional; Fluorescence Only)
(empty) position is in the light path – this position has no attenuation of fluorescence signal and is a good starting point for fluorescence observation. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 21: Power Supply
“INPUT” on the back of the mainframe. Attach the slotted end of the power cord to the power adapter, and the plug to a suitable electrical receptacle. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 22: Camera Adapter
Compare the image on the monitor with the image seen through the eyepieces, and rotate the camera until the orientation is the same. Tighten the locking screws to secure the camera orientation. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 23: Operation
(distance between the pupils of the observer’s eyes). Proper interpupillary distance results in a single uniform view of Indicator the specimen. Interpupillary distance range: 55~75mm Interpupillary distance scale ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 24: Coarse/Fine Focus
Focusing knobs are located on both sides of the microscope. The left side displays a scale, 0.10mm/division. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 25: Condenser Turret: Components
Ph3/L positions and cannot be adjusted. Change to another position on the condenser turret by rotating the turret rotation wheel. Rotate until the desired position faces front (i.e., B for brightfield). ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 26: Kohler Illumination: Aligning The Condenser In The Optical Path
Adjust condenser centration again using the centering screws. Open the field diaphragm until it is outside the field of view. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... - Page 27 75% (open). The condenser aperture position is directly related to the Numerical Aperture of the objective, and adjustment should be performed after every objective change ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 28: Condenser Turret: Centering Phase Contrast Annuli
The dark ring is fixed and located in the objective – this ring cannot be moved. Insert each of the two 1.5mm hex screwdrivers into the centering screws (left and right) of the condenser. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 29: Differential Interference Contrast (Dic): Component Adjustment
DIC condenser prism) and the analyzer are oriented at 90° to each other. Gently tighten the thumb screw on the analyzer gauge to hold the extinction position. Replace the DIC nosepiece prism. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 30: Side Port Light Path Selector
1.5X : Increases the magnification by 1.5X Magnification indicator Magnification Blank (no label) : This position is changer reserved for future feature turret development. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 31: Fluorescence Components
1. Place a specimen or piece of paper on the stage. Iris open: 2. While viewing the sample under fluorescence illumination, close the field diaphragm part way Iris closed: ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... - Page 32 Blank slider. Ensure that the blank slider is fully inserted into the slot. The detent will engage. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 33: Programming The Lcd Panel
“U” for UV or DAPI, “V” for visible or brightfield, etc.). Note that the intelligent turret does not automatically recognize the presence of a filter cube. NOP = No Object Present ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... - Page 34 Settings “gear” icon. These settings are saved even if power to the microscope is turned off. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 35: Microscope Operation
Upper light path selector Lower light path selector Condenser turret position “Ph1” / “Ph2” / “Ph3/L” Magnification changer Fluorescence cube turret (if Choose an open position (no filter cube installed) installed) ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... - Page 36 3. If necessary, reconfirm extinction of the condenser DIC component and the rotatable analyzer. See section 6.1.6. 4. If desired, change side port light path selector or trinocular tube upper light path selector to a camera port for imaging. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com...
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Page 37: Reflected Light Methods
4. Change fluorescence cube turret position to a different filter cube and observe again. 5. If desired, change side port light path selector or trinocular tube upper light path selector to a camera port for imaging. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 38: Microscopy Imaging
2. Change light path selector to desired camera port. Image should now appear on computer monitor or camera display. 3. Adjust camera settings per camera manufacturer or software developer’s recommendations. 4. Observe and/or acquire images as desired. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 39: Technical Specification
50% @ 40°C (104°F) • Pollution level: 2 • Power supply: ～220V ±10%, 50/60HZ • Consumed power: 100W • Fuse: T5A/250V, Ø 5x20mm • Air pressure: 80kPa～106kPa • Overvoltage category: II ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 40: Objective Specifications
1.8-2.6 MC/BF: sPlan- 0.25 10.5 0.45 8.1-7.0 0.60 3.6-2.8 *BF = Brightfield DIC = Differential interference contrast FL = Fluorescence PH = Phase contrast MC = Modulation contrast ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 41: Exi-600 Configuration Diagram
2. Trinocular observation tube 8. Condenser 3. Eyepiece 9. LED lamp house 4. Camera adapter 10. Transmitted illumination pillar 5. Objective 11. UV shield 6. DIC nosepiece prism ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 42: Exi-600 Fluorescence Configuration Diagram
2. Fluorescence filter cube turret 7. Aperture diaphragm slider 3. Fluorescence filter cube 8. Neutral Density filter slider 4. Analyzer slot blank slider 9. Fluorescence light source 5. Field diaphragm slider ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 43: Troubleshooting
Change to a phase contrast used for phase contrast objective. observation. The phase contrast annulus Ensure the correct phase (ring) does not match the contrast position on the ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 44: Electrical
LED. Observe the light intensity status on the LCD panel. Filters or other components in Remove filters, sliders, the light path. polarizers, etc. that are not being ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 45: Microscopy Imaging
Cover eyepieces when imaging. room light, etc. appears in merging with specimen image. Move camera to different camera camera image. port that allows 100% of light to go to the camera. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 46: Maintenance
7. Any repair or replacement of components of this product should be executed by our company or an authorized ACCU-SCOPE service provider. ACCU-SCOPE ® 73 Mall Drive, Commack, NY 11725 • 631-864-1000 • www.accu-scope.com... -
Page 47: Limited Microscope Warranty
ACCU-SCOPE distributor or ACCU-SCOPE at (631) 864-1000. This warranty is limited to the continental United States of America. All items returned for warranty repair must be sent freight prepaid and insured to ACCU-SCOPE INC., 73 Mall Drive, Commack, NY 11725 –...