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Cellink BIO X User Manual

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BIO X
User manual
CELLINK AB
Arvid Wallgrens Backe 20
Gothenburg, SE 41346
SWEDEN
1

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  • Page 1 BIO X User manual CELLINK AB Arvid Wallgrens Backe 20 Gothenburg, SE 41346 SWEDEN...
  • Page 2 Not anymore. The technology in front of you is the next step on the path of regenerative medicine. BIO X is designed with your research in mind. By simplifying the 3D bioprinting process, you can focus on your research and dream bigger than ever before.

  • Page 3: Table Of Contents

    3. Getting started............................16 3.1 Unpacking BIO X........................18 3.2 Contents ..........................19 3.3 Setting up BIO X........................20 3.4 Starting BIO X for the first time..................21 4. Bioprinting with BIO X..........................22 4.1 Clean Chamber........................24 4.2 Bioprinting from a 3D model....................25 4.3 Bioprinting droplets.......................38 4.4 Utilities............................43...

  • Page 4: Safety Information

    1. Safety information...

  • Page 6: General Safety Information

    Sweden 1.1 General safety information If BIO X acts in a way that is not described by this manual, turn it off and contact CELLINK. Use BIO X for its intended purposes only. Do not modify its instruments, subcomponents or accessories.

  • Page 7: Unpacking, Lifting And Carrying

    1.2 Unpacking, lifting and carrying Read how to safely unpack and setup BIO X in Chapter 3. BIO X is heavy. Always use a minimum of two people to unpack and move the machine. 1.3 Electrical information Always connect BIO X to a grounded socket and use the voltage described in the specifications.

  • Page 8: Hazardous Materials

    1.6 Compliance By using BIO X, you agree to the terms stated here and in the warranty (Chapter 6.) Except for the express warranty set forth in the warranty information and in our software license terms as applicable, all equipment and software provided hereunder are provided “as is,”...

  • Page 9: Electrostatic Discharge Protection

    • Any metal surface in a laminar flow hood. • Any metal sink. • The frame of the BIO X when connected to a grounded power outlet, as well as any exposed screws, including the screws on the left-side door hinges.

  • Page 10: Specifications

    2. Specifications...

  • Page 12: Product Map

    2.1 Product map 1. Clean Chamber ™ unit 2a. UV sterilization LED 1 2b. UV sterilization LED 2 3a. Printhead mount 1 3b. Printhead mount 2 3c. Printhead mount 3 4a. Photo-curing module mount 1 4b. Photo-curing module mount 2 5.
  • Page 13 4 5 6 1. Power switch 2. Fuse 3. Power socket 4. USB port 5. Ethernet port 6. Pneumatic port...

  • Page 14: Technical Specifications

    2.2 Technical specifications Power input: 100-240 VAC, 50-60 Hz, 600 W Fuse: 250VAC, T8A Air pressure input: 0-700 kPa Weight: 17 kg Height: 477 mm Width: 441 mm Depth: 365 mm Filter class: HEPA 14 Operate BIO in a room with a temperature between 20 and 23 degrees Celsius. In normal operating conditions, the chamber temperature will rise between 2 and 3 degrees, and the standard pneumatic printheads will rise between 4-5 degrees.

  • Page 16: Getting Started

    3. Getting started...

  • Page 18: Unpacking Bio X

    Lift the packaging carefully to turn the BIO X so it is standing on its correct side. Remove the top piece of the packaging. Grab it at the bottom edge to lift BIO X out of the bottom piece of packaging. Place BIO X on the table.

  • Page 19: Contents

    [1x] Material safety data sheet and Application note..............[2x] 3 mL CELLINK PLURONICS bioink ...................... [1x] Material safety data sheet and Application note..............[2x] 3 mL CELLINK GelMA C bioink (sterile)....................[1x] Material safety data sheet and Application note..............[2x] 3 mL CELLINK GelMA A bioink (sterile)....................

  • Page 20: Setting Up Bio X

    (see 2.1 Product map) and the grounded wall socket with the included power cable. BIO X has an internal air supply capable of supplying 200 kPa to the printheads. To use a higher pressure, connect an external air supply to the back of BIO X (see 2.1 Product map.) Turn on BIO using the power switch on the back (see 2.1 Product map.)

  • Page 21: Starting Bio X For The First Time

    3.4 Starting BIO X for the first time When you start BIO X for the first time, you will be prompted to review a license agreement. After accepting the agreement, you will be taken to the home screen. Standby – Tap to put BIO X in standby mode. To start BIO X from standby mode, turn off the power using the power switch on the back, wait 30 seconds and turn it back on.

  • Page 22: Bioprinting With Bio X

    4. Bioprinting with BIO X...

  • Page 24: Clean Chamber

    4.1 Clean Chamber Sterilizing with UV light Do not look directly at UV light and do not expose your skin to UV light. UV light can cause serious personal injury. Attention! The UV sterilization procedure will kill or damage any living organisms inside the print chamber.

  • Page 25: Bioprinting From A 3D Model

    Note: The USB drive needs to be readable and writeable. Next, choose the model you wish to bioprint. BIO X accepts the file formats .stl, .obj, .amf, and .BIOX. File list - Tap to select the model you wish to bioprint.
  • Page 26 4.2.2 Print surface Select the surface to bioprint on. BIO X can bioprint on a range of surfaces, including petri dishes, well plates and glass slides. Choose the surface and indicate the surface size. Petri dish - Select to bioprint on a petri dish.
  • Page 27 Toggle printhead – Tap to indicate if you want to use the printhead or not. If you select the printhead, you will be prompted with more settings. Bioink profile – Select the bioink profile you want to use. BIO X comes preloaded with profiles for all CELLINK bioinks.
  • Page 28 Calibration settings appear after selecting a printhead. Use or modify an existing profile, or create a new one. Bioink and photo-curing parameters are the same for all bioinks and printheads, but the printhead parameters change depending on the printhead selected. Nozzle type –...
  • Page 29 Adjust the print-bed temperature in the print-bed settings. Use temperature control to keep the print bed at a certain temperature, or turn temperature control off to print at room temperature. Clean Chamber fan – Turn the Clean Chamber fan on or off. Adjust temperature –...
  • Page 30 4.2.4 Layer settings Modify options to define the 3D model’s layers and how BIO X should bioprint the model. Use the predefined settings or create custom settings. Preview – View a preview of the chosen layer profile. Preview slider – Drag this to select which layer to preview.
  • Page 31 These settings enable you to use support structures while bioprinting and use multiple infill materials to create comlex structures. Three different modes are available: Infill – If enabled, BIO X will use the selected printhead to bioprint the infill pattern of your 3D model.
  • Page 32 4.2.7 Overview and mounting of bioprinting equipment Review all of your selected settings. If a setting or value is incorrect, use the navigation bar at the bottom of the screen to navigate back and correct it. Printhead 1 - Settings for printhead 1. Printhead 2 - Settings for printhead 2.
  • Page 33 Refer to the specific printhead manual for instructions on how to mount a cartridge. To mount the printhead in BIO X, move the printhead straight down toward the printhead holder. Press it until you feel the magnet snap into place. Remember to connect pneumatic printheads to the pressure connector.
  • Page 34 4.2.9 Overview and mounting of bioprinting equipment Use the spring-loaded arms to securely fasten the print surface according to the diagram below. Turn the arms to the sides when using a well plate. Ensure the outer dimensions of the well plate equal 128 x 85 millimeters.
  • Page 35 4.2.10 Calibration and Auto Bed Leveling (ABL) Navigate to the calibration settings to calibrate the printheads and surface probe. The surface probe is used for ABL. ABL probes the bed at three points to enable bed-leveling compensation. It uses a matrix to compensate for tilt in the bed or print surface.
  • Page 36 4.2.11 Print model This screen summarizes the bioprinting process. You can monitor the activity of each printhead and curing module while printing. Always pause bioprinting before opening the door. Do not remove printheads before bioprinting has stopped. Doing so risks aborting the process and compromising your cells.
  • Page 37 You can pause printing at any point. After pausing, you can resume or cancel printing. Pause – Tap to pause the bioprinting process. Resume - Tap to resume the bioprinting process. This option is only available when the bioprinting process is paused. Cancel –...

  • Page 38: Bioprinting Droplets

    G-code. BIO X guides you through the setup process. 4.3.1 Choose Print surface Select the well plate to bioprint on. BIO X can bioprint on a range of well plates, from 6 to 96 wells. Select a number to choose a well plate to print in.
  • Page 39 Toggle printhead – Tap to select if you want to use the printhead or not. If you select to use the printhead further settings will appear. Bioink profile – Select a predefined bioink profile or create a new one. BIO X comes preloaded with profiles for all CELLINK bioinks.
  • Page 40 4.3.3 Overview and mounting of bioprinting equipment Review all the print settings. If a setting or value is incorrect, navigate back and correct it using the navigation bar at the bottom of the screen. Printhead 1 - Settings for printhead 1. Test bioink flow - Press the droplet button to assess the bioink flow.
  • Page 41 4.3.4 Calibration and Auto Bed Leveling (ABL) Calibrate the printheads and surface probe. The surface probe is used for ABL. ABL probes the bed at three points to enable bed-leveling compensation. It uses a matrix to compensate for tilt in the bed or print surface. The surface probe only needs to be calibrated once.
  • Page 42 4.3.5 Print model This screen summarizes the bioprinting process. You can monitor the activity of each printhead and curing module while printing. Always pause bioprinting before opening the door. Do not remove printheads before bioprinting has stopped. Doing so risks aborting the process and compromising your cells.

  • Page 43: Utilities

    4.4 Utilities 4.4.1 Update your software version Download the software update onto a USB drive and insert the USB drive into the BIO X. Navigate to the first tab of the “Utilities” menu to access the update. USB content list – Select the software update from the list.
  • Page 44 4.4.2 Printhead and print bed temperature control Use the second tab of the “Utilities” menu to control printhead and print bed temperature. You have the option to pre-heat or pre-cool your equipment. Printhead 1 temperature – Set the temperature of the printhead 1. Printhead 2 temperature –...
  • Page 45 4.4.3 Printhead pressure control Evaluate the pressure necessary for extruding the biomaterial. Extrusion pressure depends mainly on material viscosity. Set printhead pressure. (Pressure should be between 0-200 kPa with an internal air compressor and 0-700 kPa with an external air compressor.) Test bioink flow - Press the droplet button to assess the bioink flow.
  • Page 46 4.4.4 Miscellaneous tab Use this tab to enable special features for experimental purposes. Enable dark mode - Turn off all chamber lights. Use this to work with photo-sensitive material. Clean Chamber fan – Turn the Clean Chamber fan on or off. Log printer info - Log all relevant print information.
  • Page 47 4.4.5 Printhead movement test Use this tab before printing to test printhead movement along the X, Y and Z axes. Printhead 1 - Test movement for printhead 1. Printhead 2 - Test movement for printhead 2. Printhead 3 - Test movement for printhead 3. Z axis command - Controls the position of the printbed along the Z axis.
  • Page 48 4.4.6 Customer support tabs The output data tab is used for customer support. CELLINK uses this tab to perform sensor testing. Output data - Allows customer support to check and test the different censors. License - Provides the printer and software license.

  • Page 50: Maintenance

    5. Maintenance...

  • Page 52: Changing The Hepa Filter

    5.1 Changing the HEPA filter The HEPA filter is part of CELLINK’s patented Clean Chamber™ technology, ensuring a sterile print chamber for your research. Change the HEPA filter after 160 hours of runtime using the instructions below. Turn off BIO X and disconnect the power cable.
  • Page 53 5.2 Manually cleaning or sterilizing BIO X BIO X has a chemically resistant coating. Clean or sterilize it with ethanol. Before using ethanol, turn off and unplug BIO X. Spray the surface and wipe it with a soft cloth. After using ethanol, wait at least 60 seconds before starting BIO X. Ethanol is flammable, and this ensures that all remaining ethanol vapor is ventilated.

  • Page 54: Terms And Conditions

    6. Terms and conditions...

  • Page 56: General Terms And Conditions

    Agreement or any other agreement. In the event that the Buyer at any time should fail to make payment in full on the due date CELLINK shall be entitled to claim interest on the sum overdue until payment is made at the rate equivalent to the reference rate of interest (as determined by the Central Bank of Sweden) plus eight percentage points per annum.
  • Page 57 If the Buyer does not report defects or deficiencies in Products delivered within the time limits set out above, the Buyer shall have no right to make any claims against CELLINK with regard to the default or defect.
  • Page 58 If the Buyer gives such notice as referred to above, and no defect is found for which CELLINK is liable, CELLINK shall be entitled to compensation for the work and costs which it has incurred as a result of the notice.
  • Page 59 The Buyer shall indemnify and hold CELLINK harmless to the extent that CELLINK incurs liability towards any third party in respect of loss or damage for which CELLINK is not liable in relation to the Buyer following section 9. For the avoidance of doubt, the Buyer shall have a liability to recourse all costs inflicted on CELLINK, due to any such claims.
  • Page 60 In no event shall CELLINK be liable towards the Buyer for any loss of production or profit, loss of use, loss of data, loss of contracts or for any other consequential, economic or indirect loss whatsoever in respect of the sale, purchase, use or disposition of the Product.

  • Page 61: End User Licence Agreement (Eula)

    OR UPDATES AFTER THE MODIFICATIONS HAVE BECOME EFFECTIVE SHALL BE DEEMED LICENSEE’S CONCLUSIVE ACCEPTANCE OF THE MODIFIED TERMS. CELLINK holds all right, title, and interest in and to the CELLINK 3D Bioprinter Software (“Licensed Software”) and all associated websites, platforms, and applications, and is the sole and exclusive owner of https:/ /cellink.com (the “Website”).
  • Page 62 CELLINK’s trademarks in any way, including but not limited to promoting Licensee’s products produced with a CELLINK 3D Bioprinter or Licensed Software and shall make no claims that CELLINK endorses Licensee’s products or services. Licensee recognizes and acknowledges that its breach of this User License may cause irreparable damage to CELLINK, which cannot be readily remedied by monetary damages in an action at law, and may constitute an infringement of CELLINK’s intellectual...
  • Page 63 User License. Features of the Licensed Software and CELLINK Site content may link a user to a third-party site, however, such links do not mean that CELLINK endorses or accepts responsibility for the content or use of any third-party material. Licensee should consult the policy statements of each third-party site visited.
  • Page 64 Licensed Software to develop another product or service. Licensee agrees not to interfere with or disrupt servers, networks or the hosting of data by CELLINK or its service providers, including by transmitting computer viruses or other malicious computer code, programs or files. Licensee will not engage in nor permit or assist a third party to perform any action prohibited by this User License.
  • Page 65 Licensee acknowledges and agrees that these and other moving parts may present a risk of bodily injury to a user of a CELLINK 3D Bioprinter and the Licensed Software. CELLINK IS IN NO WAY LIABLE FOR ANY BODILY INJURY, SUCH AS INJURY TO A USER’S HANDS, FOR ANY DAMAGE RESULTING FROM OPERATING A CELLINK...
  • Page 66 CELLINK in violation of the Export Control Laws. Licensee will notify CELLINK immediately if a violation of the Export Control Laws has occurred. If CELLINK determines that such violation should be disclosed to the applicable export control authority, Licensee agrees to provide assistance and information as reasonably required to facilitate such disclosure.
  • Page 67 CELLINK upon written notice of such changes or additions to Licensee. If there is a conflict between this User License and other CELLINK terms of service, the other terms will apply. Terms stipulated by Licensee that vary this User License or other terms of service are void and have no effect unless agreed to in a writing signed by CELLINK.

  • Page 68: Warranty

    We will also personally insure every bioprinter sent under Declared Value Insurance, also optioned by FedEx. If, in the case there is a problem with the BIO X outside of the FedEx terms, it is not the result of neglect of the purchaser and the incident occurs and is reported within 3 months (90 days)
  • Page 69 In the case that a repair is determined to be outside of the warranty and the buyer decides to send back the BIO X for repairs, the buyer will ship the BIO X at their own expense. The costs to repair the BIO X will also be at the buyer’s expense.

  • Page 70: Printhead Manuals

    7. Printhead manuals...

  • Page 72: Cellink Pneumatic-Based Printhead

    Opening for cartridge overview – View the volume of bioink left in the cartridge. Cartridge fastening screw – Securely fastens the cartridge to the printhead. Nozzle or needle. Printhead contact – The connector between BIO X and the intelligent printhead. Magnet – The magnet securely fastens the printhead on the printhead holder.
  • Page 73 Turn the cartridge-fastening screw to secure the cartridge in the printhead. The CELLINK pneumatic-based printhead needs to be connected to an air supply to function. When inserting the printhead, remember to connect the pressure hose to the pressure connector on the...
  • Page 74 Imagination is the beginning of creation. You imagine what you desire, you will what you imagine, and at last, you create what you will. -George Bernard Shaw Happy printing!

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