Cellink BIO X EMD User Manual
  1. Manuals
  2. Brands
  3. Cellink Manuals
  4. 3D Printers
  5. BIO X EMD
  6. User manual

Cellink BIO X EMD User Manual

Printhead
Hide thumbs
Table of Contents

Advertisement

Quick Links

Download this manual
BIO X EMD Printhead
User Manual

Advertisement

Table of Contents
loading

Related Manuals for Cellink BIO X EMD

Summary of Contents for Cellink BIO X EMD

  • Page 1 BIO X EMD Printhead User Manual...

  • Page 3: Table Of Contents

    Contents 1.Package contents 4.6 Testing Extrusion 2.Technical specifications 4.7 Your first bioprint 3.Safety Information 4.8 Removing printhead 3.1 BIO X system warnings 4.9 Printing optimization 3.2 EMD Printhead warnings 5.Relevant G-code commands 4.Getting started 6.Frequently asked questions 4.1 Unpacking 7.Maintenance 4.2 Installing, raising, lowering and uninstalling the 7.1 Cleaning printhead...

  • Page 5: Package Contents

    Package contents...
  • Page 6 01 Package contents Item Part number Quantity EMD Printhead 000000020566 EMD accessory tool 000000020557 Luer lock adaptor D16110020558 Cartridge airline adapter with transparent tube 3cc 12 cm 000000010040 EMD microvalve 000000020551 Threaded nozzle (needleless) D16110020555 Threaded nozzle (with needle) 000000020560 Needle cap 000000020556 Flushing tube with Luer adaptor, 20 cm...

  • Page 7: Technical Specifications

    Technical specifications...
  • Page 8 02 Technical specifications • Compatible with 3-mL cartridge. • Maximum bioink volume in cartridge: 3 mL. • Dimensions (height x width x depth): 118 x 30.5 x 37.5 mm. • Weight: 132 g. • Maximum pressure: 700 kPa. • Material composition: •...
  • Page 9 02 Technical specifications Figure 1: Theoretical maximum build volume shown from the top and side view using the EMD Printhead (assuming a nozzle length of 0 mm). Exact build volume depends on the cartridge’s position in the printhead and the nozzle/needle used.
  • Page 10 02 Technical specifications Table 1: Theoretical build volume based on nozzle length. Actual volume may vary based on build plate’s thickness, size, shape and level, as well as on cartridge position and tightness of the nozzle. X, Y and Z are measured from the front left corner of the printbed.

  • Page 11: Safety Information

    Safety information...

  • Page 12: Bio X System Warnings

    03 Safety information 3.1 BIO X system warnings • Please consult the BIO X manual for BIO X-specific and general warnings and safety procedures. 3.2 EMD Printhead warnings • Do not clean the printhead by submerging it in liquid or using excessive spraying. Liquid inside the printhead can damage the circuitry and motor.
  • Page 13 03 Safety information Figure 2: Do not touch the outlined area of the EMD microvalve.
  • Page 14 03 Safety information • Do NOT remove the valve, nozzle or cartridge from the EMD printhead while it is attached to the printhead mount. The cartridge will be pressurized upon attachment and untwisting these components will result in bioink leakage. •...

  • Page 15: Getting Started

    Getting started...

  • Page 16: Unpacking

    04 Getting started NOTE: The printing parameters displayed on this manual might not be accurate for your specific protocol. For recommended parameters please consult the documentation for the bioink being used. 4.1 Unpacking 1. Open the package. Remove the pre-assembled EMD Printhead and other components (Figure 3). 2.
  • Page 17 04 Getting started Figure 3: Package contents include: A. EMD Printhead body. E. Threaded nozzle. B. Microvalve. F. Luer lock adapter. C. Nozzle cover for storage. G. EMD accessory tool. D. Needleless threaded nozzle.

  • Page 18: Installing, Raising, Lowering And Uninstalling The

    04 Getting started 4.2 Installing, raising, lowering and uninstalling the printhead 1. To install the printhead in the BIO X, stabilize the bottom of the printhead mount with one hand to avoid straining the motor. Align the printhead above the desired printhead mount and push downward using your opposite hand (Figure 4). Note: for more information on how to assemble the printhead, see Section 4.3.
  • Page 19 04 Getting started 2. On the user interface, navigate to the Utilities menu and then go to the Tools submenu. 3. Lower the desired printhead mount into the active or loading position by pressing the down arrow (Figure 5). Figure 5: Lowering the printhead into the active position.

  • Page 20: Removal And Insertion Of Microvalve And Nozzle

    04 Getting started 4. Press the up arrow to return the printhead to the nonactive position. 5. To remove the printhead, use one hand to stabilize the printhead mount and the other hand to push the printhead slightly upward. 4.3 Removal and insertion of microvalve and nozzle 1.
  • Page 21 04 Getting started Figure 6: (A) Removing the nozzle cover from the EMD Printhead. (B, D, E) Removing the nozzle from the EMD Printhead. (C, F, G) Removing the microv- alve from the EMD Printhead.
  • Page 22 04 Getting started 5. Insert the adapter tool into the printhead (Figure 7 A, D). Twist the tool counterclockwise while securing the printhead with your other hand (Figure 7 B, E). Pull the tool out with the Luer lock attached (Figure 7 C, F). Figure 7: Removing the Luer lock adapter.

  • Page 23: Reassembling The Printhead

    04 Getting started 4.4 Reassembling the printhead 1. Use some force to push the microvalve into the printhead until approximately four millimeters of the valve are protruding (Figure 8 C, D). Do not apply pressure on the glass end (Section 3.2). 2.

  • Page 24: Loading A Cartridge

    04 Getting started 3. Reinsert the Luer lock adapter by reversing the steps outlined in Section 4.3 and Figure 7. Do not overtighten or the threads on the adapter may be stripped and leakage could occur. 4.5 Loading a cartridge 1.
  • Page 25 04 Getting started 3. Insert the cartridge into the EMD Printhead (without connecting the printhead to the BIO X) (Figure 10). Figure 10: Inserting and tightening the cartridge into the EMD Printhead.
  • Page 26 04 Getting started 4. Connect the cartridge to the pneumatic adapter by twisting the adapter on the end of the cartridge (Figure 11). Figure 11: Attaching the 3-mL pneumatic adapter to the cartridge.
  • Page 27 04 Getting started 5. Connect the air tubing to the respective air inlet on the printbox above the respective printhead (Figure 12). Figure 12: Attaching the EMD Printhead to the BIO X printhead mount.
  • Page 28 04 Getting started 6. Connect the printhead to the BIO X while stabilizing the mount. Follow the instructions in Section 4.2 (Figure 13). Figure 13: Connect the printhead to the BIO X.

  • Page 29: Testing Extrusion

    04 Getting started 4.6 Testing Extrusion 1. Navigate to the Utilities menu and select the Tools submenu. Figure 14: Adjusting the EMD Printhead parameters in the Utilities menu.
  • Page 30 04 Getting started 2. Select the EMD Printhead parameters button to adjust the open and cycle time (Figure 15) • Open time: The length of time the valve is open during one cycle. • Cycle time: The time between each opening that determines the droplet deposition frequency. Figure 15: Adjustable parameters for the EMD Printhead.
  • Page 31 04 Getting started 3. The pressure can be adjusted within the cartridge to control the expulsion rate of the droplet. • Certain bioinks require a minimum pressure to be expelled. • A pressure that is too high can cause an expulsion rate that is too high, resulting in splattering of the droplet on the surface. •...

  • Page 32: Your First Bioprint

    04 Getting started 4.7 Your first bioprint 1. Prepare the cartridges (Section 4.5). 2. Select Bioprint from the Start menu (Figure 16). Figure 16: Start menu. Select Bioprint to proceed print to setup.
  • Page 33 04 Getting started 3. Select an STL file (extension .stl) from the Model menu and proceed to the next menu (Figure 17). Figure 17: Selecting STL file from the Model tab.
  • Page 34 04 Getting started 4. Select a surface to print on. Proceed to the next menu (Figure 18). Figure 18: Select a print surface from the Surface menu.
  • Page 35 04 Getting started 5. Select printhead positions that have been set up. Make sure the EMD Printhead is selected under the tool type (Figure 19). 6. Enter the desired printing parameters for the printhead (Figure 19) and proceed to the Layers menu.
  • Page 36 04 Getting started 7. Assign the enabled printhead to the respective layer characteristics and proceed to the next menu (Figure 20). Figure 20: Layer menu. You can assign toolheads to respective print areas like perimeter, infill and support. You can select the infill pattern and density and preview the layers.
  • Page 37 04 Getting started 8. Prime the nozzle/needle and test bioink flow by using the Drop button next to the pressure setting (Figure 21). Press Print to proceed to the calibration page. Figure 21: Print menu. You can preview your printing parameters and test the pressure.
  • Page 38 04 Getting started 9. Select Calibrate to calibrate the system. Set the EMD Printhead to the desired printing height (Figure 22). A calibration height of 1-3 cm is recommended for the needleless threaded nozzle, and 0 mm for the needle threaded nozzle. 10.

  • Page 39: Removing Printhead

    04 Getting started 4.8 Removing printhead 1. Wipe any excess bioink from the nozzle tip using a delicate task wipe. 2. Cover the nozzle with the nozzle cover. 3. Depressurize the cartridge by detaching the airline from the printer (Figure 23A). 4.
  • Page 40 04 Getting started Figure 23: Depressurizing and removing the EMD Printhead.

  • Page 41: Printing Optimization

    04 Getting started 4.9 Printing optimization The EMD Printhead uses an electromagnetically actuated valve to form droplets in the micro- and nanoliter range. Pressurized fluid can flow when the valve is open, and the flow stops when it is closed. An optimal nozzle diameter, valve travel speed and actuation yield the minimal dispensing volume.
  • Page 42 04 Getting started Unlike pneumatic-based bioprinting, this technique results in small droplets instead of filaments. The translational speed of the bioprinting process will affect the pitch between droplets. A low speed might result in the droplets merging together or aggregating, while a high speed will result in a larger distance between droplets.

  • Page 43: Relevant G-Code Commands

    Relevant G-code commands...
  • Page 44 05 Relevant G-code commands Commands Description G1 Xnnn Ynnn Ennn Fnnn When used in combination with the G90 command, which defines absolute coordinates, G1 is the abso- lute move command. The values of the X and Y parameters are the coordinates (in mm) directing where to move.
  • Page 45 05 Relevant G-code commands G92 Xnnn Ynnn Znnn G92 sets the current position of the printhead to the specified X, Y and Z coordinates. If no values are given, the position is assumed to be 0, 0, 0 (this will also change the Z position). Tx switches to printhead (x), where 0, 1 and 2 designate printheads 1, 2 and 3.

  • Page 47: Frequently Asked Questions

    Frequently asked questions...
  • Page 48 06 Frequently asked questions • What is the mechanism that enables bioprinting with this printhead? This printhead compresses the bioink within a cartridge. The cartridge is connected to an electromagnetic valve that can open and close for a set length of time. Droplets are expelled from the printhead at the open and close rates set by the user. •...

  • Page 49: Maintenance

    Maintenance...

  • Page 50: Cleaning

    07 Maintenance 7.1 Cleaning 1. After printing, flush the microvalve, nozzle and Luer lock adapter to clean them and prevent clogging. 2. Disassemble the printhead (Section 4.2). 3. Mount the Luer lock adapter to a syringe loaded with deionized water or ethanol. 4.
  • Page 51 07 Maintenance Figure 25: Preparing the microvalve for cleaning. Figure 26: Flushing the microvalve.
  • Page 52 07 Maintenance 10. Repeat steps 7 to 8 with air instead of water/ethanol to dry the microvalve. 11. Disconnect the microvalve and let it air dry. 12. Connect the nozzle to the provided tube. Use the syringe to flush with water or ethanol, then water. Hold the nozzle tightly when flushing to prevent it from disconnecting from the tube (Figure 27).

  • Page 53: Long-Term Maintenance

    07 Maintenance 7.2 Long-term maintenance • The threaded nozzles and Luer lock adapter can be autoclaved and cleaned with ethanol. They have sufficient chemical resistance to ethanol and are temperature-resistant for autoclaving. • Regularly clean the printhead with a damp cloth to remove any dust or debris.

  • Page 55: Appendix A: Consumables- Needles And Nozzles

    Appendix A: Consumables— needles and nozzles...

  • Page 56: Available Nozzles And Part Numbers

    Appendix A: Consumables — needles and nozzles Available nozzles and part numbers • Available nozzles for contact dispensing (inner diameters): • 0.10 mm. • 0.15 mm. • 0.30 mm (standard. Art. no. D16110020555). • Available microvalves for contact and noncontact jetting. Threaded sleeve used for noncontact jetting where fluid is shot directly from microvalve end.
  • Page 57 Appendix A: Consumables — needles and nozzles Common and proven valve diameter/travel combinations: • 0.1 / 0.06 travel. • 0.1 / 0.03 travel. • 0.15 / 0.03 travel. • 0.15 / 0.06 travel. • 0.2 / 0.06 travel. • 0.2 / 0.1 travel. •...

  • Page 58: Compatible Consumables

    Appendix A: Consumables — needles and nozzles Compatible consumables CSC010300502 Empty cartridges 3cc – 50 pcs CSC010300102 Empty cartridges 3cc with end and tip caps – 10 pcs CSC010311502 Empty cartridges 3cc with end and tip caps – 50 pcs CSO010311102 UV-shielding cartridges 3cc with end and tip caps –...

  • Page 59: Support Information

    Support information • Official site: www.cellink.com • Contact: support@cellink.com • Contact: sales@cellink.com • Web store: www.cellink.com/store Store Sales Support Official site...
  • Page 60 www.cellink.com...

This manual is also suitable for:

000000020566

Table of Contents