Cellink INKREDIBLE User Manual
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Cellink INKREDIBLE User Manual

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INKREdIBLE
User manual
CELLINK AB
Arvid Wallgrens Backe 20
Gothenburg, SE 41346
1
SWEDEN

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Summary of Contents for Cellink INKREDIBLE

  • Page 1 INKREdIBLE User manual CELLINK AB Arvid Wallgrens Backe 20 Gothenburg, SE 41346 SWEDEN...
  • Page 2 This manual will serve as your guide for setting up your INKREDIBLE 3D Bioprinter, maintaining it, and also notifying you about safety precautions to ensure a long-lasting product lifetime.

  • Page 3: Table Of Contents

    1.1 General safety information 1.2 Protective equipment 1.3 Liquids and dispensing media 1.4 Opening the product and service 2. Introduction to your INKREDIBLE 3D Bioprinter 2.1 Specifications 3. Unpacking your INKREDIBLE 3D Bioprinter 3.1 Contents of the box 4. Product map 5.

  • Page 4: Safety Information

    1. Safety information...

  • Page 6: General Safety Information

    Before you start using your INKREDIBLE 3D bioprinter, please take time to review the entire manual. Maloperation of the INKREDIBLE 3D bioprinter instrument can lead to severe and irreversible injuries if the safety instructions are not followed. Throughout the manual we will use the following two symbols to indicate if there is risk for equipment damage or personal injury: WARNING! This symbol will indicate if there is risk of personal injury.

  • Page 7: Protective Equipment

    1.4 Opening the product and service WARNING! The INKREDIBLE 3D Bioprinter SHOULD NOT be taken apart. Doing so endangers both the users and the equipment! The INKREDIBLE 3D Bioprinter IS NOT user servicable! Opening of the instrument’s electrical panel will void the...

  • Page 8: Introduction To Your Inkredible 3D Bioprinter

    2. Introduction to your INKREDIBLE 3D Bioprinter...

  • Page 10: Specifications

    LCD controller or it can be monitored through a computer with the use of the accompanied software. Your three-dimensional CAD models are translated into coordinates and instructions for the INKREDIBLE to allow the bioprinter to move according to a defined path. The bioprinting process works through the extrusion of a bioink or hydrogel biomaterial combined with human cells in a bottom- up, layer-by-layer fashion until a 3D construct is built.
  • Page 11 US/MEX/CA/JAP/Taiwan: 110VAC, 60Hz INKREDIBLE 3D Bioprinter: Input: 24 VDC, 6 A 2.1.5 IP-classification IP10 CAUTION! If you want to use another air supply than the one provided by CELLINK, please contact us to avoid damage to your INKREDIBLE 3D Bioprinter.

  • Page 12: Unpacking Your Inkredible 3D Bioprinter

    3. Unpacking your INKREDIBLE 3D Bioprinter...
  • Page 14 Once the INKREDIBLE 3D Bioprinter has been carefully taken out of the box, all protective films, zip ties and plastic parts preventing the axes to move during transportation can be removed. Once all protective materials have been removed, the INKREDIBLE can be placed at its intended place of operation (for example inside a biological hood or laboratory bench).

  • Page 15: Contents Of The Box

    [1x] Air supply box containing: [1x] 150 W, 300 kPa portable, oil-free air compressor [1x] STARTINK-kit containing: [1x] Cell syringe [1x] Prefilled syringe with 3 mL of CELLINK Bioink [1x] Dispensing unit [1x] Mixing unit [1x] Filling cartridge [2x] 5 ml of crosslinking solution...

  • Page 16: Product Map

    4. Product map...
  • Page 18 INKREdibLE 5a 5b 6 13 14 1 1a 1 1b...
  • Page 19 On/Off Button [2a] Printhead 1 (Ph 1) [2b] Printhead 2 (Ph 2) UV LED Printbed [5a] Digital pressure monitor for printhead 1 [5b] Digital pressure monitor for printhead 2 SD-card reader [7a] Printbed adjustment butterfly screw [7b] Printbed adjustment butterfly screw [7c] Printbed adjustment butterfly screw Control knob...

  • Page 20: Operating Your Inkredible 3D Bioprinter

    5. Operating your INKREDIBLE 3D Bioprinter...

  • Page 22: Setting Up For First Time Use

    5.1.2 Supplying Power and Air 1. Plug the power cord into the back [11] of the of the INKREDIBLE 3D Bioprinter and then into the power outlet. 2. Connect the air tube (provided) to the air compressor and then to the back of the INKREDIBLE 3D bioprinter [12].
  • Page 23 6. Turn the control knob [8] to select “Prepare Bioprint” and press to enter. 7. Turn control knob [8] to “Home Axes” and press it to select. The INKREDIBLE 3D bioprinter will now home all axes. The nozzle of printhead 1 should be in the middle of the printbed once the sequence is completed.
  • Page 24 6. Adjust the air pressure to your desired flow by turning the pressure regulator for printhead 1 [11a] (for CELLINK Start and a red nozzle, 28 kPa gives a good flow of printmass). 7. When you have aquired your desired flow, press the control knob to turn off the printhead (the printhead will turn on automatically when a bioprint is initiated).

  • Page 25: Starting A Print

    5.2.5 Starting the print: 1. The INKREDIBLE 3D Bioprinter is now ready to bioprint! Start by inserting your SD- card, containing the 3D model you want to print, into the SD-card reader [6]. 2. In the menu, turn the control knob [8] to “Bioprint” and press to enter. If the “Bioprint”...

  • Page 26: Advanced Features

    If you wish you can crosslink bioprints using the built-in UV LED crosslinking system of the INKREDIBLE 3D Bioprinter. To do so follow these steps: 1. Manually place the UV LED [3] as close as possible to the top of the bioprinted construct.

  • Page 28: How To Install And Setup Heartware

    6. How to install and setup HeartWare...

  • Page 30: Installing Heartware (Pc)

    • Refer to the electronic version of this manual, click the following link, navigate to “CELLINK HeartWare for PC” and download “setupHeartWare_2_4_1.zip. • Go to the CELLINK Support page at https:/ /cellink.com/support/, click “Downloads” and select “Download” next to HeartWare. 2. Open the ZIP archive and run “setupHeartWare_2_4_1.exe”.
  • Page 31 5. Click “Next” after the next three prompts unless you need to customize: •The users for which Python is installed •The path in which Python is installed •The components of the Python package you want installed However, leaving the default options is strongly recommended. 6.
  • Page 32 8. Click “Finish” to complete the installation. 6.1.1 Uninstalling HeartWare Sometimes, issues with the functionality of HeartWare can be resolved by completely uninstalling and reinstalling HeartWare. In addition, to update HeartWare, the old version, must be completely uninstalled. 1. Click the “Start” icon on the bottom left corner of your desktop.
  • Page 33 4. Scroll through the list till you find “Cellink HeartWare version 2.4.1”. 5. Press “Yes” on the following three prompts, and HeartWare will be uninstalled. This will not uninstall Python 2.6 or remove your Slic3r settings.

  • Page 34: Installing Repetier-Host (Mac)

    Repetier-Host for Mac software. 1. Navigate to the Repetier-Host page by either: - Go to the CELLINK Support page at https:/ /cellink.com/support/, click “Downloads” and select “Download” next to Repetier-Host. - Go to the Repetier-Host site “https:/ /repetier.com/download-now/” and select “Repetier-Host Mac 1.10”.
  • Page 35 3. Paste it into the “Applications” folder in Finder. 4. Open the “Repetier-Host Mac” file to start the install process. A security message may appear because the Repetier-Host Application is from an “unrecognized developer”.
  • Page 36 5. Open the “System Preferences”. 6. Go to “Security & Privacy”.
  • Page 37 7. Click “Open Anyway”. 8. Click “Open”...
  • Page 38 9. Open Repetier and read through the “First Steps” to familiarize yourself with the app. Then, follow the setup instructions in “6.3 Setting up HeartWare/Repetier Host to configure your software to your INKREDIBLE.

  • Page 39: Setting Up Heartware

    6.3 Setting up HeartWare/Repetier Host 1. When HeartWare starts for the first time, select “Skip this Version” 2. Start by clicking on “Printer Settings” 3. Copy the settings in the images below for any INKREDIBLE series bioprinter...
  • Page 41 4. Once you are done, press “Apply,” then click “OK”.

  • Page 42: Slicing With Heartware

    7. Slicing with HeartWare...

  • Page 44: What Is Slicing

    7.1.2 Slices The INKREDIBLE is a pneumatically driven bioprinter that extrudes material in thin strands. To build a model, the INKREDIBLE must extrude a series of strands in distinct paths such that the printed material looks like the model desired.

  • Page 45: Heartware Interface

    CELLINK bioprinters Connect – Connect your INKREDIBLE to your machine to control movements or run prints. Note: Your INKREDIBLE must be connected securely via the provided USB cable for this to work, and “SD Print” should be disabled from the “Utilities” menu on the INKREDIBLE.
  • Page 46 Toggle Log – Hide and unhide the Log [5] window. Show Filament – Hide and unhide the appearance of printed material in generated G-Code within the View Window [3]. Show Travel – Hide and unhide the appearance of printer movement with no extrusion (non-print moves) in generated G-Code within the View Window [3].
  • Page 47 7.2.3 View Window The View Window allows you to perform several functions: Examine the size and position of models, relative to the print volume. Examine the results of transformations performed within HeartWare. Preview G-Code, as interpreted by HeartWare, for quick verification of accurate printer commands.
  • Page 48 The same visualization tools in the Print Preview are also available here. Manual Control – If an INKREDIBLE is connected via a USB cable and the Printer Settings are correctly configured, many actions can be performed from this window such as movement, needle calibration, and temperature control (the LCD menu must be used to assign a temperature first).

  • Page 49: Basic Slicing Workflow

    7.3 Basic Slicing Workflow 7.3.1 Staging Your Models Before you can create code, you must place models, in the form of .STL or .OBJ files, within the print volume, then scale and spatially arrange them to suit your application. 1. To add files you may: •...
  • Page 50 Export – Once one or models have been positioned in a desired arrangement, they can be saved for later use. This can be useful f the same arrangement will be sliced at different thickness, run with different infill, or built with different materials.
  • Page 51 The default, “INKREDIBLE” will work for most materials. Filament Setting – With respect to the INKREDIBLE, this solely provides temperature information to the Slic3r for materials that require different temperatures for printing or when printing with cells.
  • Page 52 2. When you have selected the settings that align with your needs, click “Slice with Slic3r”. The software will automatically switch to the “Print Preview” tab and the View window will look like the image below. The blue lines depict the path that the printhead will follow to extrude print your part. You can use the sliders under “Visualization”...
  • Page 53 3. The the model sliced, and the G-Code generated, now you can decide how the file gets printed: Print – If an INKREDIBLE is connected to the PC, you can run the print directly with this button. See 5.2.5 “Starting the Print” for how to print directly from a PC.

  • Page 54: Maintenance And Support

    8. Maintenance and troubleshooting...

  • Page 56: Maintaining Your Inkredible 3D Bioprinter

    Verify you get the grease inside of the threads themselves. 4. Grasp both sides of the printbed and move it to the top of the INKREDIBLE (be careful not to get any grease on the printbed).
  • Page 57 8. Lubricate the exposed segments of the carriage rods . Be careful not to spill any lubricant onto the baseplate of the printer. 9. Gently pull the X-carriage all the way to the front. 10. Gently push the printhead carriage all the way to the right. 11.

  • Page 58: Support For Your Inkredible 3D Bioprinter

    8.2 Support for your INKREDIBLE 3D Bioprinter Please visit CELLINK’s Support Portal for updated information on your INKREDIBLE 3D bioprinter. www.cellink.freshdesk.com/ www.cellink.freshdesk.com/support/solutions/35000068779 8.2.1 One or both of the printheads are not dispensing ink or not dispensing ink in a continuous flow The issue is most likely that the nozzle(s) are clogged.
  • Page 59 G-code file. To fix this, do the following: 1. Open up your G-code file in Notepad or a similar program. 2. Check if the line: ”Post processed by INKREDIBLE post processor ” is written at the top of the document.
  • Page 60 Follow these steps to find the probing position for each axis: 1. Navigate to the “Utilities Menu” and enter by pressing the control knob [8]. 2. Select HOME AXES and let the INKREDIBLE 3D Bioprinter carry out the sequence. If printhead 1 does not trigger the probing sensor, trigger it manually by hand by pressing the sensor 3 times when the needle is about to touch the sensor.
  • Page 61 6. Return to the “Utilities Menu” and select the option “Set Probe Position” by pressing the control knob [8]. 7. Restart the INKREDIBLE 3D Bioprinter. 8.2.11 Pressure monitor not displaying correct pressure The issue is most likely that the 0-level for pressure has been offset. To fix this, do the following: 1.

  • Page 62: Warranty And Guarantee

    9. Warranty and guarantee...
  • Page 64 We will also personally insure every bioprinter sent under Declared Value Insurance, also optioned by FedEx. If, in the case there is a problem with the INKREDIBLE outside of the FedEx terms, it is not the result of neglect of the purchaser and the incident occurs and...
  • Page 65 In the case that a repair is determined to be outside of the warranty and the buyer decides to send back the INKREDIBLE for repairs, the buyer will ship the INKREDIBLE at their own expense. The costs to repair the INKREDIBLE will also be at the buyers expense.
  • Page 66 You ship it back at your own expense and after we receive it, we will deduct a 10% restocking fee from the total refund of the price of the INKREDIBLE 3D Bioprinter (excluding the tax and other fees). There will also be a thorough inspection of the bioprinter, and upon finding any damages deemed on the part of the buyer, other deductions from the refund would be made accordingly.
  • Page 68 “You cannot create new science unless you realize where the old science leaves off and new science begins, and science-fiction forces us to confront this.” - Michio Kaku...

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