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Cellink BIO X Installation And Quick Start Manual

Autocalibration kit
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BIO X Autocalibration Kit
Installation and Quick-start Guide
The Autocalibration Kit enables Automatic Bed Leveling (ABL) and automatic X, Y and Z
calibration. These features are available on HeartOS software version 1.7 and above.
The BIO X autocalibration removes the potential for user error when calibrating the tip of the
nozzle relative to the printing surface or to another nozzle. It does this by probing and aligning
all printhead nozzle tips in all axes (X, Y and Z), and includes ABL.
ABL measures the print bed surface and adjusts all movements to follow its tilt and contours to
improve the quality of bioprinted structures. ABL compensates for any height differences
present in a print bed or printing surface. After a brief calibration procedure, the print bed
adjusts the Z height while running the bioprinting protocol, ensuring the construct is level
relative to the printing surface. ABL requires a surface probe to automatically measure the
print bed's height at various points.
Boston, U.S.
Gothenburg, Sweden
Arvid Wallgrens Backe 20,
100 Franklin St.，
Gothenburg, 41346
Boston, MA 02110
Virginia, U.S.
2000 Kraft Dr., Suite 2125
Blacksburg, VA 24060
www.cellink.com | +1 (833) CELLINK
Kyoto, Japan
46-29 Yoshida-Shimo Adachi-
cho, Sakyo-ku, Kyoto
1

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Summary of Contents for Cellink BIO X

  • Page 1 These features are available on HeartOS software version 1.7 and above. The BIO X autocalibration removes the potential for user error when calibrating the tip of the nozzle relative to the printing surface or to another nozzle. It does this by probing and aligning all printhead nozzle tips in all axes (X, Y and Z), and includes ABL.
  • Page 2 BIO X Autocalibration Kit parts Part Quantity Surface probe Surface probe stabilizer Surface probe magnet Z-station base Z-station plate XY printhead calibration lever Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.，...
  • Page 3 NOTE: Before installing the BIO X Autocalibration Kit, make sure that your BIO X is running HeartOS 1.7 or above. If the HeartOS software installed on the BIO X is version 1.6 or older, your system will not be compatible with the Autocalibration Kit.
  • Page 4 X and Y endstops. Verify that the status light turns blue to indicate a status change for the X-probe and Y-probe. NOTE: If any endstop components are misaligned, absent or not activating, contact the CELLINK Support team at support@cellink.com. Kyoto, Japan Virginia, U.S.
  • Page 5 Installation of BIO X Autocalibration Kit Step 1: Installing the surface probe station 1. Turn the BIO X off and remove all printheads. 2. Unscrew the bottom mechanical endstop screw of the second printhead, labeled by the green circle in the diagram.
  • Page 6 Step 3: Installing the XY printhead calibration lever 1. Turn the BIO X on and navigate to the Utilities menu. Select Printer, Hardware then Sensors. 2. Align the left and right edges of the XY...

  • Page 7: Machine Calibration

    Utilities -> Move and calibrate -> Surface probe -> Calibrate Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 8 Z direction. Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 9 Figure 4 Incorrect calibration position. Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 10 Figure 5. Remove the probe when autocalibration is not running. Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 11 Enabling automatic bed leveling (ABL) for a print WARNING. Do NOT leave BIO X unsupervised while it runs ABL. Be prepared to unplug BIO X if ABL fails. Leaving BIO X unsupervised during the ABL process risks damage to your machine.
  • Page 12 NOTE: Keeping the probe attached while printing may damage the printed construct. Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 13 Enabling automatic calibration for a print WARNING. Do NOT leave BIO X unsupervised while it runs automatic calibration. Be prepared to unplug BIO X in the event of automatic calibration failure. Leaving BIO X unsupervised during the automatic calibration process risks damage to your machine.
  • Page 14 8. Select Start to begin the calibration process. Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 15 Kyoto, Japan Virginia, U.S. Boston, U.S. Gothenburg, Sweden 2000 Kraft Dr., Suite 2125 46-29 Yoshida-Shimo Adachi- Arvid Wallgrens Backe 20, 100 Franklin St.， Blacksburg, VA 24060 cho, Sakyo-ku, Kyoto Gothenburg, 41346 Boston, MA 02110 www.cellink.com | +1 (833) CELLINK...
  • Page 16 Removing hardware for packing When packing BIO X for transport, remove the following parts: • XY printhead calibration lever. • Z station base. • Surface probe. Tools are not required to remove these parts. Ensure they are stored safely during transport.